Молекулярный и генетический анализ некоторых семейств гликозил-гидролаз микроорганизмов

выводы.

1. У молочнокислых бактерий Lactobacillus plantarum NRRL В-4496 и Pediococcus pentosaceus NRRL В-11 465 обнаружена сходная структура локуса утилизации сахарозы: дивергентно расположенные ген scrA, кодирующий сахарозо-специфичный второй фермент фосфотрансферазной системы, и гены scrB, scrR и agi, кодирующие ß—фруктозидазу, регуляторный белок и а-глюкозидазу соответственно. Сходство 5'-концевых частей генов scrB у L. plantarum и P. pentosaceus достигает 98.6%.

2. Проведено картирование в субтеломерных районах хромосом Saccharomyces cerevisiae девяти полимерных генов ферментации мелибиозы: ген MEL2 локализован в левом плече седьмой хромосомы (VII L), MEL3 — в XVI L, MEL4 — в XI L, MEL5 — в IV L, MEL6 — в XIIIR, MELI — в VI R, MEL8 — в XV R, MEL9 — в X R и MEL10 — в XIIR.

3. У штамма S. cerevisiae CBS 2888 обнаружено три гена ферментации мелибиозы MEL12-MEL14, представляющие новое дивергентное семейство а-галактозидазных генов. Проведено картирование этих генов в субтеломерных районах левых плечей хромосом S. cerevisiae: MELI2 в — IX, MEL13 — в XV и MEL14 — в X.

4. Обнаружена закономерность локализации полимерных генов семейств утилизации углеводов у S. cerevisiae. Гены ферментации сахарозы, мальтозы и мелибиозы, принадлежащие одному семейству (SUC, MAL, MELI-MELI 1 или MEL12-MEL14), никогда не находятся в противоположных концах одной и той же хромосомы (даже в разных штаммах).

5. Обнаружена гомология аминокислотных последовательностей ß—фруктозидаз, принадлежащих двум разным семействам гликозил-гидролаз: GH32 и GH68. Эти два семейства объединены в ß—фруктозидазное суперсемейство.

6. Обнаружена гомология белков ß—фруктозидазного суперсемейства с ß—D-ксилозидазами и a-L-арабинофуранозидазами семейств GH43 и GH62 гликозил-гидролаз. Оба семейства включены в состав ß—фруктозидазного суперсемейства. В состав этого суперсемейства также включена группа гипотетических белков, вероятно, кодирующих гликозидазы.

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