Π ΠΎΠ»Ρ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΠΈ Π ΠΠ-Π±Π΅Π»ΠΊΠΎΠ²ΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
ΠΠ°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° Π±ΡΠ»Π° Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π° Π½Π° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΈΡ ΡΠΎΠ»ΠΈ Π² ΡΡΠΎΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠ΅. ΠΡΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΠΈ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΡΠΊΠ°Π·Π°Π½ΠΈΡ Π½Π° ΡΠΎΠ»Ρ ΡΠ±ΠΈΠΊΠΈΠ²ΠΈΡΠΈΠ½-ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. ΠΠΎΡΡΠΎΠΌΡ ΠΌΡ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΡΡΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π½Π° ΠΈΠΌΠΏΠΎΡΡ Π’Π Π1. ΠΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΠΈΠ· ΡΠ΅ΡΡΠΈ… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ
- 1. 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ
- 1. 1. 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Tetrahymena
- 1. 1. 2. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΡΠΈΠΏΠ°Π½ΠΎΡΠΎΠΌΠ°ΡΠΈΠ΄
- 1. 1. 2. Π°. ΠΡΡΡΠ΄ Kinetoplastidae
- 1. 1. 2. 6. ΠΡΡΡΠ΄ Leishmania
- 1. 1. 3. ΠΡΡΠ³ΠΈΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»ΠΈ ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ
- 1. 2. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 1. 3. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ
- 1. 4. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
- 1. 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ
- 2. Π£Π±ΠΈΠΊΠ²ΠΈΡΠΈΠ½-ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ²
- 2. 1. ΠΠ²Π΅Π΄Π΅Π½ΠΈ Π΅
- 2. 2. Π€ΡΠ½ΠΊΡΠΈΠΈ Π£ΠΠ‘ Π² ΠΊΠ»Π΅ΡΠΊΠ΅
- 2. 3. Π£Π±ΠΈΠΊΠ²ΠΈΡΠΈΠ½ΠΈΠ»ΠΈΡΡΡΡΠ°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° Π² S. cerevisiae
- 2. 4. 26S ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ°: ΡΡΡΠΎΠ΅Π½ΠΈΠ΅ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 2. 4. 1. 20. S ΡΡΠ±ΡΠ°ΡΡΠΈΡΠ°, ΠΈΠ»ΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΄ΡΠΎ
- 2. 4. 2. 19S ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½Π°Ρ ΡΡΠ±ΡΠ°ΡΡΠΈΡΠ°
- 2. 5. ΠΠ΄Π΅ Π»ΠΎΠΊΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Ρ ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΡ Π² ΠΊΠ»Π΅ΡΠΊΠ΅?
- 2. 6. Π£Π·Π½Π°Π²Π°Π½ΠΈΠ΅ ΠΏΠΎΠ»ΠΈΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½ΠΎΠ²ΡΡ
ΡΠ΅ΠΏΠ΅ΠΉ
- 2. 6. 7. ΠΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ½ΡΠ΅ ΡΡΠ±ΡΠ΅Π΄ΠΈΠ½ΠΈΡΡ, ΡΠ·Π½Π°ΡΡΠΈΠ΅ ΠΏΠΎΠ»ΠΈΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΡΠ΅ΠΏΠΈ
- 2. 6. 2. ΠΠ ΠΈ Π4 ΡΠ΅ΡΠΌΠ΅Π½ΡΡ Π² Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ² ΠΊ ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ΅
- 2. 6. 3. ΠΠΎΡΡΠ°Π²ΠΊΠ° ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ² ΠΊ ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ΅ ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²ΠΎΠΌ Cdc48 ΠΈ Π΅Π³ΠΎ ΠΊΠΎΡΠ°ΠΊΡΠΎΡΠΎΠ²ΠΠͺ
- 2. 6. 4. Π¨Π°ΠΏΠ΅ΡΠΎΠ½Ρ ΠΈ ΠΈΡ ΠΊΠΎΡΠ°ΠΊΡΠΎΡΡ Π² Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ²
- 2. 7. UFD ΠΏΡΡΡ. «' '. DOA1 ΠΊΠ°ΠΊ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ UFD-nymu
- 2. 8. ΠΠ΅ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΎΠ»Ρ ΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½Π° ΠΈ ΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ
Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ΅
- 2. 8. 1. ΠΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ½ΠΎΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ²
- 2. 8. 2. Π‘Π²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ½ΠΎΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½-ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΠΌΠΈ Π΄ΠΎΠΌΠ΅Π½Π°ΠΌΠΈ
- 2. 8. 3. ΠΠ»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΈ ΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½Π°
- 2. 9. ΠΠ½Π΄ΡΠΊΡΠΈΡ Π£ΠΠ‘ ΠΈ ΠΊΠ°ΡΠΊΠ°Π΄ΠΎΠ² ΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΊΠΈΠ½Π°Π· Π² ΠΎΡΠ²Π΅Ρ Π½Π° ΡΠ΅ΠΏΠ»ΠΎΠ²ΠΎΠΉ ΡΠΎΠΊ
- 1. 1. Π¨ΡΠ°ΠΌΠΌΡ Escherichia col
- 1. 2. Π¨ΡΠ°ΠΌΠΌΡ Saccharomyces cerevisiae
- 1. 3. ΠΠΈΠ½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 4. 1. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ (ΠΠ¦Π )
- 4. 2. Π Π°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΠΠ ΡΠ½Π΄ΠΎΠ½ΡΠΊΠ»Π΅Π°Π·Π°ΠΌΠΈ ΡΠ΅ΡΡΡΠΈΠΊΡΠΈΠΈ ΠΈ Π΄Π΅ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 4. 3. ΠΠΈΠ³ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 4. 4. ΠΠ±ΡΠ°ΡΠ½Π°Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΠΈ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ
- 11. 1. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Ρ-Π³Π°Π»Π°ΠΊΡΠΎΠ·ΠΈΠ΄Π°Π·Ρ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Π½Π° Π½ΠΈΡΡΠΎΡΠ΅Π»Π»ΡΠ»ΠΎΠ·Π½ΡΡ ΡΠΈΠ»ΡΡΡΠ°Ρ
- 11. 2. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Ρ-Π³Π°Π»Π°ΠΊΡΠΎΠ·ΠΈΠ΄Π°Π·Ρ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠ° X-gal Π² 0.5% Π°Π³Π°ΡΠ΅
- 14. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ
- 14. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π ΠΠ
- 20. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°, ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΠ³ΠΎ Π½Π°ΠΏΡΠ°Π²Π»ΡΡΡ ΠΈΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 20. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 20. 3. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠΌΠΏΠΎΡΡΠ° Π ΠΠ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ
- 24. 1. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ 358-ΠΌΠ΅ΡΠ΅Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ²
- 24. 2. ΠΠΌΠΏΠΎΡΡ 358-ΠΌΠ΅ΡΠ΅Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 29. 4. Western-Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ BN-PAGE
- 31. 1. ΠΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΠΠΠ
- 31. 2. Π Π°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ² ΡΡΠΈΠΏΡΠΈΠ½ΠΎΠΌ Π² Π³Π΅Π»Π΅
- 31. 3. MALDI- TOF Π°Π½Π°Π»ΠΈΠ·
- 1. 1. ΠΠΎΠΈΡΠΊ Π±Π΅Π»ΠΊΠΎΠ², Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΡ
Ρ pre-Msklp Π² Π΄Π²ΡΠ³ΠΈΠ±ΡΠΈΠ΄Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅
- 1. 1. 1. ΠΠ΅ΡΠΎΠ΄ Π΄Π²ΡΠ³ΠΈΠ±ΡΠΈΠ΄Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π² Π΄ΡΠΎΠΆΠΆΠ°Ρ
- 1. 1. 2. Π‘ΠΊΡΠΈΠ½ΠΈΠ½Π³ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊ ΠΊΠΠΠ Π΄Π»Ρ ΠΏΠΎΠΈΡΠΊΠ° Π±Π΅Π»ΠΊΠΎΠ², ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ pre-Msklp
- 1. 2. ΠΠΎΠΈΡΠΊ Π±Π΅Π»ΠΊΠΎΠ², Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΡ Ρ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠΎΠΉ ΡΠ ΠΠ
- 1. 3. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠΎΠ»ΠΈ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ°
- 1. 3. 1. Π ΠΎΠ»Ρ ΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½-ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ
- 1. 3. 2. ΠΠ½Π°Π»ΠΈΠ· ΡΠΎΠ»ΠΈ Π΄ΡΡΠ³ΠΈΡ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ°
- 2. 1. ΠΠ½Π°Π»ΠΈΠ· Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π΅Π½ΠΎΠ»Π°Π·Ρ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ in vivo Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Western-Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ
- 2. 2. ΠΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΠΏΠΎ2Ρ, ΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠΉ YFP, Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 2. 3. ΠΠ½ΠΎΠ»Π°Π·Π°, ΡΠ²ΡΠ·Π°Π½Π½Π°Ρ Ρ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΡΠΌΠΈ, ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΎΠΉ?
Π ΠΎΠ»Ρ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΠΈ Π ΠΠ-Π±Π΅Π»ΠΊΠΎΠ²ΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΡΡ ΡΠΈΡΠΎΠΊΠΈΠΉ ΡΠΏΠ΅ΠΊΡΡ ΡΡΠ½ΠΊΡΠΈΠΉ, Π²ΠΊΠ»ΡΡΠ°Ρ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ½Π΅ΡΠ³ΠΈΠ΅ΠΉ Π·Π° ΡΡΠ΅Ρ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ»Ρ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΎ Π½Π°Π»ΠΈΡΠΈΠ΅ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΠΠ ΠΈ ΡΠΈΡΡΠ΅ΠΌΡ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° Π±Π΅Π»ΠΊΠ°, ΠΎΠ΄Π½Π°ΠΊΠΎ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΡΠΈΡΠ»ΠΎ ΠΌΠ°ΠΊΡΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ» ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ Π² ΠΎΡΠ³Π°Π½Π΅Π»Π»Ρ ΠΈΠ· ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΡ. ΠΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π² ΡΡΠ΄Π΅ ΡΠ»ΡΡΠ°Π΅Π² Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΏΠΎΡΡΠΈΡΡΡΡΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Π±Π΅Π»ΠΊΠΈ, Π½ΠΎ ΡΠ°ΠΊΠΆΠ΅ ΠΈ ΡΠ ΠΠ. Π Π½Π°ΡΡΠΎΡΡΠ΅ΠΌΡ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΈΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π±ΡΠ» ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ Ρ ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ , ΡΡΠ΄Π° ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ, ΡΡΠΌΡΠ°ΡΡΡ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ , Π° ΡΠ°ΠΊΠΆΠ΅ Ρ ΠΏΠΎΡΠΊΡΡΡΠΈΡ ΡΡ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Saccharomyces cerevisiae. ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΈ Π΅Π³ΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΎΡΠ»ΠΈΡΠ°Π΅ΡΡΡ ΠΎΡ Π²ΠΈΠ΄Π° ΠΊ Π²ΠΈΠ΄Ρ.
Π ΡΠ»ΡΡΠ°Π΅ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΈΠ· ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ Π΄Π²Π° Π²ΠΈΠ΄Π° ΡΠ ΠΠ — ΡΠ ΠΠΠ¬ΡΡΠΈΠΈ (Martin et al., 1979) ΠΈ ΡΠ ΠΠΡ1ΠΏ (Reinehardt et al., 2005). ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ ΡΠ²Π»ΡΠ΅ΡΡΡ Π²ΡΡΠΎΠΊΠΎΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠΌ, ΡΠ°ΠΊ ΠΊΠ°ΠΊ ΠΎΠ΄Π½Π° ΠΈΠ· Π΄Π²ΡΡ ΠΈΠ·ΠΎΠ°ΠΊΡΠ΅ΠΏΡΠΎΡΠ½ΡΡ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΡ ΡΠ ΠΠ (ΡΠ ΠΠ yscΠ°ΠΏΡΠ°Π²Π»ΡΠ΅ΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ Π²ΡΠΎΡΠ°Ρ (ΡΠ ΠΠΠ¬Ρ>ΠΈΠΈ) ΠΏΡΠΈΡΡΡΡΡΠ²ΡΠ΅Ρ ΡΠΎΠ»ΡΠΊΠΎ Π² ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ΅. Π’Π°ΠΊΠΎΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΠ΅ Π² Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π΄ΠΎΠ²ΠΎΠ»ΡΠ½ΠΎ Π±Π»ΠΈΠ·ΠΊΠΈΡ ΡΠ ΠΠ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΎΠ±ΡΡΡΠ½Π΅Π½ΠΎ ΠΎΡΠ»ΠΈΡΠΈΡΠΌΠΈ Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΡΡΠΈΡ ΡΠ ΠΠ (Entelis et al., 1998). Π‘Π»Π΅Π΄ΡΡΡΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΎΠΏΠΎΡΡΠ΅Π΄ΠΎΠ²Π°Π½ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ, Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΠΌΠΈ Ρ ΡΠ ΠΠ. Π Π°Π½Π΅Π΅ Π² Π½Π°ΡΠ΅ΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π΄Π»Ρ ΠΈΠΌΠΏΠΎΡΡΠ° Π’Π Π1 Π΄ΠΎΠ»ΠΆΠ½Π° Π±ΡΡΡ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π»ΠΈΠ·ΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π·Ρ, Π·Π°ΡΠ΅ΠΌ Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΠΎ Π΅Π΅ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Ρ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠΌ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π»ΠΈΠ·ΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π·Ρ (pre-Msklp) (Tarassov et al., 1995b). ΠΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΡ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π’Π Π1 ΠΈ pre-Msklp Π½Π΅ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ Π΄Π»Ρ ΠΈΠΌΠΏΠΎΡΡΠ° Π’Π Π1 Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ, ΡΡΠΎ Π³ΠΎΠ²ΠΎΡΠΈΡ ΠΎ Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ², Π½Π°ΠΏΡΠ°Π²Π»ΡΡΡΠΈΡ Π΅Π΅ ΠΈΠΌΠΏΠΎΡΡ, ΡΠ°ΠΊ Π½Π°Π·ΡΠ²Π°Π΅ΠΌΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ°.
ΠΠ°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° Π±ΡΠ»Π° Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π° Π½Π° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΈΡ ΡΠΎΠ»ΠΈ Π² ΡΡΠΎΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠ΅. ΠΡΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΠΈ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΡΠΊΠ°Π·Π°Π½ΠΈΡ Π½Π° ΡΠΎΠ»Ρ ΡΠ±ΠΈΠΊΠΈΠ²ΠΈΡΠΈΠ½-ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. ΠΠΎΡΡΠΎΠΌΡ ΠΌΡ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΡΡΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π½Π° ΠΈΠΌΠΏΠΎΡΡ Π’Π Π1.
ΠΠΎ Π²ΡΠ΅ΠΌΡ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π² Π½Π°ΡΠ΅ΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ Π΄Π°Π½Π½ΡΠ΅, ΡΠΊΠ°Π·ΡΠ²Π°ΡΡΠΈΠ΅ Π½Π° ΡΡΠ°ΡΡΠΈΠ΅ Π³Π»ΠΈΠΊΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° — Π΅Π½ΠΎΠ»Π°Π·Ρ — Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ (Π.ΠΠ½ΡΠ΅Π»ΠΈΡ, Π½Π΅ΠΎΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅). Π’Π°ΠΊΠΆΠ΅ Π½Π΅Π΄Π°Π²Π½ΠΎ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π΅Π½ΠΎΠ»Π°Π·Π° Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ΅ Ρ Π΄ΡΡΠ³ΠΈΠΌΠΈ Π³Π»ΠΈΠΊΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΠΌΠΈ ΠΏΡΠΈΡΡΡΡΡΠ²ΡΠ΅Ρ Π²ΠΎ ΡΡΠ°ΠΊΡΠΈΠΈ Π²Π½Π΅ΡΠ½ΠΈΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ Arabidopsis thaliana (Giege et al., 2003). ΠΠΎΠΌΠΈΠΌΠΎ ΡΡΠΎΠ³ΠΎ ΠΈΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ Π³Π»ΠΈΠΊΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΠ°Π΄ΠΎΠΌ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΡΡ ΡΡΠ½ΠΊΡΠΈΠΉ. ΠΠΎΡΡΠΎΠΌΡ ΠΌΡ Π³, Π ΠΈ ΠΊ ΡΠ»ΠΈΡ. (Π Π΅ ΠΠ³Π΅Π½ΡΠΎΠ² ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΠ»ΠΈ, ΡΡΠΎ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΡΡΠ½ΠΊΡΠΈΠΉ β’ —Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°/ΠΌΠΎΠ³Π»ΠΎ Π±Ρ Π±ΡΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ ΠΌΡ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΏΠΎΠ΄ΡΠΎΠ±Π½Π΅Π΅ ΡΠΎΠ»Ρ Π΅Π½ΠΎΠ»Π°Π·Ρ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ, Π΅Π΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²ΠΈΠ»ΠΈ ΠΏΠΎΠΈΡΠΊ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ², ΠΎΠ±ΡΠ°Π·ΡΡΡΠΈΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ Ρ Π΅Π½ΠΎΠ»Π°Π·ΠΎΠΉ.
Π¦Π΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΎΠ»ΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΠΈ Π ΠΠ-Π±Π΅Π»ΠΊΠΎΠ²ΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ.
ΠΠ»Ρ ΡΡΠΎΠ³ΠΎ Π² Ρ ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π»ΠΎΡΡ Π²ΡΠΏΠΎΠ»Π½ΠΈΡΡ ΡΠ»Π΅Π΄ΡΡΡΠΈΠ΅ Π·Π°Π΄Π°ΡΠΈ:
1. ΠΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ Π±Π΅Π»ΠΊΠΎΠ²ΡΠ΅ ΡΠ°ΠΊΡΠΎΡΡ, ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΠ΅ Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ, ΡΠ°ΠΊ Π½Π°Π·ΡΠ²Π°Π΅ΠΌΡΠ΅ ΡΠ°ΠΊΡΠΎΡΡ ΠΈΠΌΠΏΠΎΡΡΠ°, Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π΄Π²ΡΠΈ ΡΡΠΈΠ³ΠΈΠ±ΡΠΈΠ΄Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌ Π² Π΄ΡΠΎΠΆΠΆΠ°Ρ .
2. ΠΠ·ΡΡΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π½Π° ΠΈΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ.
3. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ ΡΠΎΠ»Ρ Π³Π»ΠΈΠΊΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° — Π΅Π½ΠΎΠ»Π°Π·Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ — Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ.
4. ΠΡ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°ΡΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π΅Π½ΠΎΠ»Π°Π·Ρ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ, Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΠ΅ Ρ Π΅Π½ΠΎΠ»Π°Π·ΠΎΠΉ.
ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«.
Π²ΡΠ²ΠΎΠ΄Ρ.
1. ΠΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ 9 Π±Π΅Π»ΠΊΠΎΠ², Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΡ Ρ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ ΠΈ Π΅Π΅ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΡΠΈΠΊΠΎΠΌpre-Msklp, ΠΈ ΡΠ²Π»ΡΡΡΠΈΡ ΡΡ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΠ°ΠΊΡΠΎΡΠ°ΠΌΠΈ ΠΈΠΌΠΏΠΎΡΡΠ° Π’Π Π1.
2. ΠΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΠΈΠ· ΡΠ΅ΡΡΠΈ ΠΏΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π³Π΅Π½ΠΎΠ² (DOAI, LEU9, ΠΠ’Π1, PILI, PNC1, RPN13) ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠΌΠΏΠΎΡΡΠ° Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ. ΠΡΠΎ ΠΌΠΎΠΆΠ΅Ρ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π° ΡΠΎ, ΡΡΠΎ ΠΎΠ½ΠΈ ΡΠ²Π»ΡΡΡΡΡ ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ°ΠΌΠΈ ΠΈΠΌΠΏΠΎΡΡΠ°.
3. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΠΏΡΡΠΌΠ°Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠ΅ΠΉ ΠΏΡΠΎΡΠ΅Π°ΡΠΎΠΌΡ 26S ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ.
4. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π³Π»ΠΈΠΊΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ΅ΡΠΌΠ΅Π½Ρ Π΅Π½ΠΎΠ»Π°Π·Π° 2 ΡΠ°ΡΡΠΈΡΠ½ΠΎ Π»ΠΎΠΊΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½ Π½Π° Π²Π½Π΅ΡΠ½Π΅ΠΉ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π΅ Π² ΡΠΎΡΡΠ°Π²Π΅ ΠΌΠ°ΠΊΡΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°, Π²ΠΊΠ»ΡΡΠ°ΡΡΠ΅Π³ΠΎ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΡ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΡ ΡΠ ΠΠ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ.
5. ΠΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ, ΡΡΠΎ Π΅Π½ΠΎΠ»Π°Π·Π° 2 ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΠ΅Ρ Ρ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ Π½Π΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎ ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π΅Ρ ΡΡΠ°ΡΡΠΈΠ΅ Π² Π΅Π΅ ΠΈΠΌΠΏΠΎΡΡΠ΅.
6. Π Π΅ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½Π° ΡΠΈΡΡΠ΅ΠΌΠ° ΠΈΠΌΠΏΠΎΡΡΠ° Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΈΠ· ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ².
Π‘ΠΏΠΈΡΠΎΠΊ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
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- Π― Ρ ΠΎΡΡ ΠΏΠΎΠ±Π»Π°Π³ΠΎΠ΄Π°ΡΠΈΡΡ Π‘ΠΈΠ»ΡΠ²ΠΈ Π€ΡΠΈΠ°Π½Ρ Π·Π° ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ ΡΡΠ°ΠΌΠΌΡ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΡΠ΅Π½Π½ΡΠ΅ ΡΠΎΠ²Π΅ΡΡ ΠΏΡΠΈ ΠΏΠ»Π°Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ².
- ΠΠ½Π΅ ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΈΡΡΠ½ΠΎ Π²ΡΡΠ°Π·ΠΈΡΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ Π²ΡΠ΅ΠΌ ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠ°ΠΌ ΠΊΠ°ΡΠ΅Π΄ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΉ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΠΎΡΠΊΠΎΠ²ΡΠΊΠΎΠ³ΠΎ ΠΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π£Π½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ° ΠΈ Π²ΡΠ΅ΠΌΡ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠ²Ρ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π Π―Π2735 ΠΏΠΎΠ΄ ΡΡΠΊΠΎΠ²ΠΎΠ΄ΡΡΠ²ΠΎΠΌ Π . ΠΠ°ΡΡΠΈΠ½Π° Π·Π° ΠΎΠΊΠ°Π·Π°Π½Π½ΡΡ ΠΏΠΎΠΌΠΎΡΡ, Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΠΈ ΡΠ΅ΠΏΠ»ΠΎΠ΅ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅.