Идентификация и картирование участков ДНК, специфически связывающихся с ядерным матриксом, на хромосоме 19 человека

Основные результаты и выводы.

1. Методом селекции геномных последовательностей, предпочтительно связывающихся с ядерным матриксом, получены и картированы на хромосоме 19 человека 28 новых S/MAR-элементов.

2. Идентифицирован и картирован на хромосоме 19 человека повторяющийся S/MAR-элемент, связанный с семейством генов канцероэмбриональных антигенов (CEA-MAR). Высказано предположение о том, что дупликация генов семейства может происходить по границам петлевых доменов хроматина.

3. Реконструирована доменная структура локуса хромосомы 19 человека длиной 1 млн.п.о., расположенного между маркерами D19S208 и СОХ7А1. S/MAR-элементы подразделяют локус на 10 доменов длиной от 6 до 272 т.п.о. Средний размер домена составляет 88 т.п.о.

4. Семь S/MAR-элементов находятся в интронах генов MAG, APLP1, HSPOX1, NPHS1, CACNA1A и PIN1. Эти гены, кроме PIN 1, экспрессируются тканеспецифично и связаны с наследственными заболеваниями. Такое расположение S/MARs позволяет предположить их участие в регуляции экспрессии данных генов.

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