Π§Π°ΡΡΠΎΡΠ° ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² ΠΏΡΠΈ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ escherichia coli Π-12 ΠΈ ΡΡΡΡΠΊΡΡΡΠ° Π±Π΅Π»ΠΊΠ° reca
ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ ΡΠΈΡΠ»Ρ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ², ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΡΡΠΈΡ Π² ΠΆΠΈΠ²ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠ΅. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Π½Π°ΡΠ°Π»ΠΎΡΡ Ρ ΠΎΡΠΊΡΡΡΠΈΡ Π² 1965 Π³Π΅Π½Π° Π³Π΅ΡΠ, ΠΈΠ³ΡΠ°ΡΡΠ΅Π³ΠΎ ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Escherichia coli (Clark & Margulies, 1965). ΠΠ΅Π»ΠΎΠΊ RecA ΡΡΠ°ΡΡΠ²ΡΠ΅Ρ ΡΠ°ΠΊΠΆΠ΅ ΠΈ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ, ΡΠ΅ΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ, ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π°, ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ Π΄Π΅Π»Π΅Π½ΠΈΡ, ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- Π‘ΠΏΠΈΡΠΎΠΊ ΡΠΎΠΊΡΠ°ΡΠ΅Π½ΠΈΠΉ
- ΠΠ»Π°Π²Π° 1. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. 1. ΠΠ΅Π»ΠΎΠΊ RecAB Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ
- 1. 1. 1. ΠΠ°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½Π°Ρ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΡ
- 1. 1. 2. ΠΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½Π°Ρ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ Ρ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ
- 1. 1. 3. Π¦Π΅Π½ΡΡΠ°Π»ΡΠ½Π°Ρ ΡΠΎΠ»Ρ Π±Π΅Π»ΠΊΠ° RecA Π² Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ
- 1. 1. 4. Π‘ΡΡΡΠΊΡΡΡΠ° Π±Π΅Π»ΠΊΠ° RecA ΠΈΠ· Π. coli (RecAEc)
- 1. 1. 5. Π§Π°ΡΡΠΎΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² Π½Π° Π΅Π΄ΠΈΠ½ΠΈΡΡ Π΄Π»ΠΈΠ½Ρ ΠΠΠ
- 1. 2. SOS-ΡΠΈΡΡΠ΅ΠΌΠ° Ρ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ
- 1. 2. 1. Π ΠΎΠ»Ρ Π±Π΅Π»ΠΊΠ° RecA Π² SOS-ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ
- 1. 2. 2. ΠΡΡΠ°ΡΠΈΠΈ Π³Π΅Π½Π° Π³Π΅ΡΠ ΠΈΠ· Π. coli (Π³Π΅ΡΠΠΡ) Ρ ΠΊΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΡΠΌ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ
- 1. 3. RecA-ΠΏΠΎΠ΄ΠΎΠ±Π½ΡΠ² Π±Π΅Π»ΠΊΠΈ Ρ ΠΏΡΠΎΠΊΠ°ΡΠΈΠΎΡ
- 1. 4. Π‘ΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π±Π΅Π»ΠΊΠ° RecA Ρ ΠΏΠΎΠΌΠΎΡΡΡ Ρ ΠΈΠΌΠ΅Ρ
- 1. 1. ΠΠ΅Π»ΠΎΠΊ RecAB Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ
- ΠΠ»Π°Π²Π° 2. ΠΠΠ’ΠΠ ΠΠΠΠ« Π ΠΠΠ’ΠΠΠ«
- 2. 1. ΠΠ°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΡΡΠ°ΠΌΠΌΡ ΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ
- 2. 2. Π‘ΡΠ΅Π΄Ρ
- 3. ΠΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΈ Ρ ΠΠΠ
- 2. 4. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ²
- 2. 5. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π²Π½ΡΡΡΠΈΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ Π±Π΅Π»ΠΊΠ° RecA
- 2. 6. Π Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΠΉ ΡΠ΅ΡΡ
- 2. 7. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΡΡΠΎΡΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ²
- 2. 8. Π Π΅ΠΏΠ°ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΡΠ΅ΡΡ
- 2. 9. W-ΡΠ΅Π°ΠΊΡΠΈΠ²Π°ΡΠΈΡ ΡΠ°Π³Π° A. CI
- 2. 10. SOS-Ρ ΡΠΎΠΌΠΎΡΠ΅ΡΡ
- 2. 11. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΎΡΠΈΡΡΠΊΠ° Π±Π΅Π»ΠΊΠ° LexA ΠΈΠ· Π. col
- 2. 12. Π Π΅Π°ΠΊΡΠΈΡ ΡΠ°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΡ Π±Π΅Π»ΠΊΠ° LexA
- 2. 13. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΠΠ
- 3. ΠΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΈ Ρ ΠΠΠ
- 3. 1. Π‘ΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π³Π΅Π½ΠΎΠ² Π³Π΅ΡΠ ΠΈΠ·
- 3. 1. 1. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½Π° Π³Π΅ΡΠ ΠΈΠ· Serratia marcescens Sb (recASm)
- 3. 1. 2. Π‘ΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π³Π΅Π½ΠΎΠ² recAsm ΠΈ recAEc
- 3. 1. 3. Π‘ΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π±Π΅Π»ΠΊΠΎΠ² RecAsm ΠΈ RecAEc
- 3. 1. 4. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ Π³Π΅Π½Π° recASm
- 3. 2. ΠΠΎΠ²ΡΡΠ΅Π½Π½Π°Ρ ΡΠ°ΡΡΠΎΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² (Π§Π Π), ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΡΡ Π±Π΅Π»ΠΊΠΎΠΌ RecA ΠΈΠ· Pseudomonas aeruginosa Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. col
- 3. 2. 1. ΠΠ΅Π»ΠΎΠΊ RecApa ΠΏΠΎΠ²ΡΡΠ°Π΅Ρ Π§Π Π Π±Π΅Π· ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ SOS-ΡΠΈΡΡΠ΅ΠΌΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. col
- 3. 2. 2. ΠΡΡΠ°ΡΠΈΡ 1Π΅Ρ ΠΠ Π½Π΅ Π²Π»ΠΈΡΠ΅Ρ Π½Π° ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ Π§Π Π, ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΡΡ Π±Π΅Π»ΠΊΠΎΠΌ RecApa
- 3. 2. 3. ΠΠ΅Π»ΠΎΠΊ RecAPa ΡΡΠΈΠΌΡΠ»ΠΈΡΡΠ΅Ρ ΡΠ°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠ° LexAEc
- 3. 2. 4. ΠΠ΅Π»ΠΎΠΊ SSB ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΠ΅Ρ ΡΠ΅Π°ΠΊΡΠΈΡ ΡΠ°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΡ Π±Π΅Π»ΠΊΠ° LexA
- 3. 3. Π Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Ρ ΠΈΠΌΠ΅ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² RecA (P. aeruginosa/ Π. coli)
- 3. 3. 1. Π§Π°ΡΡΠΎΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ², ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΡΡ Ρ ΠΈΠΌΠ΅ΡΠ½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ
- 3. 3. 2. ΠΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΠΎΠ΅ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΡΡ Ρ ΠΈΠΌΠ΅ΡΠ½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ
- 3. 4. ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° ΡΠ°ΡΡΠΎΡΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ²
- 3. 4. 1. ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ
- 3. 4. 2. Π€ΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ
- 4. 1. ΠΠ±Π»Π°ΡΡΠΈ Π±Π΅Π»ΠΊΠ° RecA, Π·Π°ΠΌΠ΅Π½Ρ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ Π² ΠΊΠΎΡΠΎΡΡΡ Π½Π΅ Π²Π»ΠΈΡΡΡ Π½Π° Π§Π Π
- 4. 2. ΠΠ±Π»Π°ΡΡΠΈ Π±Π΅Π»ΠΊΠ° RecA, Π·Π°ΠΌΠ΅Π½Ρ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ Π² ΠΊΠΎΡΠΎΡΡΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡ ΠΊ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ Π§Π Π, ΡΠΎΠΏΡΡΠΆΠ΅Π½Π½ΠΎΠΌΡ Ρ ΠΊΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΡΠΌ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ
- 4. 3. ΠΠ±Π»Π°ΡΡΠΈ Π±Π΅Π»ΠΊΠ° RecA, Π·Π°ΠΌΠ΅Π½Ρ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ Π² ΠΊΠΎΡΠΎΡΡΡ Π²Π»ΠΈΡΡΡ Π½Π° Π§Π Π Π² ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ ΠΊΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΈΡ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ
Π§Π°ΡΡΠΎΡΠ° ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² ΠΏΡΠΈ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ escherichia coli Π-12 ΠΈ ΡΡΡΡΠΊΡΡΡΠ° Π±Π΅Π»ΠΊΠ° reca (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ ΡΠΈΡΠ»Ρ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ², ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΡΡΠΈΡ Π² ΠΆΠΈΠ²ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠ΅. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Π½Π°ΡΠ°Π»ΠΎΡΡ Ρ ΠΎΡΠΊΡΡΡΠΈΡ Π² 1965 Π³Π΅Π½Π° Π³Π΅ΡΠ, ΠΈΠ³ΡΠ°ΡΡΠ΅Π³ΠΎ ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Escherichia coli (Clark & Margulies, 1965). ΠΠ΅Π»ΠΎΠΊ RecA ΡΡΠ°ΡΡΠ²ΡΠ΅Ρ ΡΠ°ΠΊΠΆΠ΅ ΠΈ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ, ΡΠ΅ΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ, ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π°, ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ Π΄Π΅Π»Π΅Π½ΠΈΡ, ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠ΄Π° ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ Π³Π΅Π½ΠΎΠ² (Roca & Π‘ΠΎΡ , 1997). Π€ΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ ΡΠΎΠ»Ρ Π±Π΅Π»ΠΊΠ° RecA Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅Ρ Π΅Π³ΠΎ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΠΎΡΡΡ Π² ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ. RecA-ΠΏΠΎΠ΄ΠΎΠ±Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ Π²ΠΎ Π²ΡΠ΅Ρ ΡΡΠ΅Ρ ΡΠ°ΡΡΡΠ²Π°Ρ ΠΆΠΈΠ²ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ: Bacteria, Eukarya ΠΈ Archaea (Kowalczykowski et al., 1994).
Π‘ΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π±Π΅Π»ΠΊΠ° RecA, ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΡΠΉ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΡΡ ΠΌΠΈΡΠ°, ΠΏΡΠΈΠΎΠ±ΡΠ΅Π» Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠΈΡΠ°Π½ΠΈΡ Π² 1992 ΡΡΠ΅Ρ ΠΌΠ΅ΡΠ½ΠΎΠΉ ΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΡΡΠΊΡΡΡΡ Π±Π΅Π»ΠΊΠ° (Story et al., 1992; Story & Steitz, 1992). ΠΡΠ° ΡΡΡΡΠΊΡΡΡΠ° Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ Π±ΠΈΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π΄Π°Π½Π½ΡΠΌΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° Π°Π²ΡΠΎΡΠ°ΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΡΠ°ΠΉΠΎΠ½Ρ Π±Π΅Π»ΠΊΠ° RecA, ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΡΠ΅ Π΄Π»Ρ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ Ρ ΠΠΠ ΠΈ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠΌΠΈ ΠΊΠΎΡΠ°ΠΊΡΠΎΡΠ°ΠΌΠΈ, ΠΌΠ΅ΠΆΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ Ρ ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ°ΠΌΠΈ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½Π°Ρ ΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΡΠΊΡΡΡΠ° Π»Π΅Π³Π»Π° Π² ΠΎΡΠ½ΠΎΠ²Ρ ΠΈΠ½ΡΠ΅ΡΠΏΡΠ΅ΡΠ°ΡΠΈΠΈ Π΄Π°Π½Π½ΡΡ ΠΎ Π±ΠΈΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ²ΠΎΠΉΡΡΠ²Π°Ρ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² RecA.
ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΠΎΡΠ΅Π²ΠΈΠ΄Π½ΡΠ΅ ΡΡΠΏΠ΅Ρ ΠΈ Π² ΠΈΠ·ΡΡΠ΅Π½ΠΈΠΈ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π±Π΅Π»ΠΊΠ° RecA, Π΄ΠΎ ΡΠΈΡ ΠΏΠΎΡ ΠΎΡΡΠ°Π΅ΡΡΡ ΠΎΡΠΊΡΡΡΡΠΌ Π²ΠΎΠΏΡΠΎΡ ΠΎ Π΅Π³ΠΎ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ: ΠΊΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠΎΠ² Π±Π΅Π»ΠΊΠ° RecA ΠΈ ΠΊΠ°ΠΊΠΎΠ² ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Π±Π΅Π»ΠΊΠ° Ρ ΠΠΠ Π² Π½ΡΠΊΠ»Π΅ΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠ²ΠΎΠΌ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΠ΅.
ΠΡΠΎΡ Π²ΠΎΠΏΡΠΎΡ ΠΏΡΠΈΠΎΠ±ΡΠ΅Π» ΠΎΡΠΎΠ±ΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ Π² ΡΠ²Π΅ΡΠ΅ Π΄Π°Π½Π½ΡΡ ΠΎΠ± ΡΠ½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½ΠΎΡΡΠΈ ΡΡΡΡΠΊΡΡΡΡ Π½ΡΠΊΠ»Π΅ΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΠ°, ΠΎΠ±ΡΠ°Π·ΡΠ΅ΠΌΠΎΠ³ΠΎ RecA-ΠΏΠΎΠ΄ΠΎΠ±Π½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ. Yu ΠΈ Egelman (1993Π°) ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½ΠΎΡΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΠΎΠ², ΠΎΠ±ΡΠ°Π·ΡΠ΅ΠΌΡΡ Π½Π° ΠΠΠ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ RecA, Rad51 ΠΈ UvsX. Rad51 ΡΠ²Π»ΡΠ΅ΡΡΡ Π°Π½Π°Π»ΠΎΠ³ΠΎΠΌ Π±Π΅Π»ΠΊΠ° RecA Π² Π΄ΡΠΎΠΆΠΆΠ°Ρ Saccharomyces cerevisiae (Shinohara et al., 1992; Ogawa Π’. et al., 1993), a UvsX — Π² Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Π΅ Π’4 (Yu & Egelman, 1993Π°). Π’ΠΎΡ ΡΠ°ΠΊΡ, ΡΡΠΎ RecA-ΠΏΠΎΠ΄ΠΎΠ±Π½ΡΠ΅ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΡ Π½Π°ΠΉΠ΄Π΅Π½Ρ Ρ ΡΡΠΊΠ°ΡΠΈΠΎΡ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ, ΡΡΠΎ ΠΏΡΠΈΡΠΎΠ΄Π° ΡΡΠΎΠ³ΠΎ Π½ΡΠΊΠ»Π΅ΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΉ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΠΎΠ±ΠΌΠ΅Π½Π° Π½ΠΈΡΠ΅ΠΉ ΠΌΠ΅ΠΆΠ΄Ρ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠΌΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π°ΠΌΠΈ ΠΠΠ Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ΅Π½ Π΄Π»Ρ ΠΏΡΠΎΠΈ ΡΡΠΊΠ°ΡΠΈΠΎΡ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π΄Π°Π½Π½ΡΠ΅, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΏΡΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° RecA, ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½Ρ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ ΠΈ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ Ρ Π²ΡΡΡΠΈΡ , Π²ΠΊΠ»ΡΡΠ°Ρ ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°.
ΠΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΡΠΈΠ»ΠΈΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Ρ Π½Π° Π±ΠΈΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ ΠΏΡΡΠ΅ΠΌ ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ Π΅Π΅ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ ΡΡΠ°ΠΏΠΎΠ² in vitro. ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Π²Π½ΠΎΡΠΈΡ Π²Π΅ΡΠΎΠΌΡΠΉ Π²ΠΊΠ»Π°Π΄ Π² ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° ΡΡΠΎΠ³ΠΎ ΡΠ»ΠΎΠΆΠ½Π΅ΠΉΡΠ΅Π³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° in vivo.
Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΏΠΎΡΡΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Escherichia coli Π-12. ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΠΎΠΉ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ RecA ΠΈΠ· Π. coli, Serratia marcescens, Pseudomonas aeruginosa, Π° ΡΠ°ΠΊΠΆΠ΅ Ρ ΠΈΠΌΠ΅ΡΠ½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ RecA (Π. coli / P. aeruginosa).
ΠΠ°Π΄Π°ΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π²ΠΊΠ»ΡΡΠ°Π»ΠΈ:
1) ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π³Π΅Π½Π° recASm ΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π±Π΅Π»ΠΊΠΎΠ² RecASm ΠΈ RecAEc.
2) ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΊΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ ΠΈ ΡΠ°ΡΡΠΎΡΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² (Π§Π Π) ΠΏΡΠΈ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ.
3) ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΡΠ²ΠΎΠΉΡΡΠ² Π±Π΅Π»ΠΊΠ° RecAPa Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli.
4) ΠΠΎΠΈΡΠΊ ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ Π² ΡΡΡΡΠΊΡΡΡΠ΅ Π±Π΅Π»ΠΊΠ° RecA, Π²Π»ΠΈΡΡΡΠΈΡ Π½Π° Π§Π Π.
ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π° Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½Π°Ρ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π³Π΅Π½Π° recASm ΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡ ΡΡΠΎΠ³ΠΎ Π³Π΅Π½Π° ΠΌΠΎΠΆΠ΅Ρ ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ Π·Π°ΠΌΠ΅ΡΠ°ΡΡ Π±Π΅Π»ΠΎΠΊ RecAEc Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli ΠΠ³Π΅ΡΠ. ΠΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½Ρ Π³ΠΈΠΏΠ΅ΡΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΎΠ³Π΅Π½Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π±Π΅Π»ΠΊΠ° RecA ΠΈΠ· P. aeruginosa Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠ½ΠΈ Π½Π΅ ΡΠ²ΡΠ·Π°Π½Ρ Ρ ΠΈΠ½Π΄ΡΠΊΡΠΈΠ΅ΠΉ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli SOS-ΡΡΠ½ΠΊΡΠΈΠΉ. ΠΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΡΡΡ, ΡΡΠΎ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΠ° Π±Π΅Π»ΠΊΠ° RecA ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌ, ΠΏΡΠ΅Π΄ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠΌ ΡΠ°ΡΡΠΎΡΡ ΠΈΠ½ΠΈΡΠΈΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² ΠΏΡΠΈ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ.
ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π΄ΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΠΈ ΠΎΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½Ρ Π² ΡΠ΅ΡΡΡΠ΅Ρ ΠΏΠ΅ΡΠ°ΡΠ½ΡΡ ΡΠ°Π±ΠΎΡΠ°Ρ , Π±ΡΠ»ΠΈ Π΄ΠΎΠ»ΠΎΠΆΠ΅Π½Ρ Π½Π° ΡΠ΅ΠΌΠΈΠ½Π°ΡΠ°Ρ Π² Π£Π½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ΅ Π³. ΠΡΠ°ΠΊΠ° (Π―ΠΏΠΎΠ½ΠΈΡ, 1996) ΠΈ ΠΠΈΡΠΊΠΎΠ½ΡΠΈΠ½ΡΠΊΠΎΠΌ Π£Π½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ΅ Π‘Π¨Π (Π³. ΠΠ΅Π΄ΠΈΡΠΎΠ½, 1998) ΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ Π½Π° ΠΠΆΠ΅Π³ΠΎΠ΄Π½ΡΡ ΠΎΡΡΠ΅ΡΠ½ΡΡ ΠΊΠΎΠ½ΡΠ΅ΡΠ΅Π½ΡΠΈΡΡ , ΠΎΡΠ³Π°Π½ΠΈΠ·ΡΠ΅ΠΌΡΡ HHMI Π² 1996;1998 Π³Π³, Π° ΡΠ°ΠΊΠΆΠ΅ Π½Π° ΠΊΠΎΠ½ΡΠ΅ΡΠ΅Π½ΡΠΈΠΈ «ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ ΠΈ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΠ½ΡΠ΅ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΈ» Π² Π‘Π½ΠΎΡΠΌΠ°ΡΡ ΠΠΈΠ»ΠΈΠ΄ΠΆ, ΠΠΎΠ»ΠΎΡΠ°Π΄ΠΎ (Π‘Π¨Π, 1999).
ΠΠ«ΠΠΠΠ«.
1. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ ΠΈ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ Π³Π΅Π½ Π³Π΅ΡΠ ΠΈΠ· S. marcescens Sb (recASm). ΠΠ³ΠΎ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½Π°Ρ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΎΠΊΠ°Π·Π°Π»Π°ΡΡ Π½Π° 82.4% ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½Π° ΡΡΡΡΠΊΡΡΡΠ΅ Π³Π΅Π½Π° Π³Π΅ΡΠΠΡ. Π Π΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ «-10», «-35» ΠΈ SOS-Π±Π»ΠΎΠΊ Π² Π³Π΅Π½Π΅ recAsm ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½Ρ (Π·Π° ΠΈΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π° — Π·Π°ΠΌΠ΅Π½Π° G (-20) Π½Π° Π‘ Π² SOS-Π±Π»ΠΎΠΊΠ΅) ΡΠ°ΠΊΠΎΠ²ΡΠΌ Π² Π³Π΅Π½Π΅ Π³Π΅ΡΠΠΡΒ¦ ΠΠ΅Π½ recASm ΡΠΏΠΎΡΠΎΠ±Π΅Π½ ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ Π·Π°ΠΌΠ΅Π½ΡΡΡ Π³Π΅Π½ Π³Π΅ΡΠΠΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli, ΠΊΠΎΠΌΠΏΠ΅Π½ΡΠΈΡΡΡ ΡΠ΅-ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΠΉ, ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΈ SOS Π΄Π΅ΡΠ΅ΠΊΡΡ ΡΡΠ°ΠΌΠΌΠ° Π. coli ΠΠ³Π΅ΡΠ.
2. ΠΡΡΠ²Π»Π΅Π½Π° ΠΏΠΎΠ²ΡΡΠ΅Π½Π½Π°Ρ ΡΠ°ΡΡΠΎΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² Π½Π° Π΅Π΄ΠΈΠ½ΠΈΡΡ Π΄Π»ΠΈΠ½Ρ ΠΠΠ (Π§Π Π), ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΠ°Ρ Π±Π΅Π»ΠΊΠΎΠΌ RecAPa Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli. ΠΠ½Π° Π½Π΅ Π·Π°Π²ΠΈΡΠ΅Π»Π° ΠΎΡ ΠΊΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΈΡ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ.
3. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΊΠΎΠ½ΡΡΠΈΡΡΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ SOS-ΡΡΠ½ΠΊΡΠΈΠΉ ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ Π§Π Π Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. coli.
4. ΠΠ½Π°Π»ΠΈΠ· ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΡΠ²ΠΎΠΉΡΡΠ² Ρ ΠΈΠΌΠ΅ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² RecApa/RecAec ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ Π·Π°ΠΌΠ΅Π½Ρ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ, ΠΏΡΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ°ΡΠΈΡ ΡΡΠ°ΡΡΠΊΠ°ΠΌ RecA-RecA Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈΠ»ΠΈ ΠΏΡΠΈΠ»Π΅ΠΆΠ°ΡΠΈΡ ΠΊ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌ, Π²Ρ ΠΎΠ΄ΡΡΠΈΠΌ Π² ΡΡΠΈ ΡΡΠ°ΡΡΠΊΠΈ, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½Ρ Π·Π° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π§Π Π. ΠΡΠΎ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅Ρ, ΡΡΠΎ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΠ° Π±Π΅Π»ΠΊΠ° RecA ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌ, ΠΏΡΠ΅Π΄ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠΌ ΡΠ°ΡΡΠΎΡΡ ΠΈΠ½ΠΈΡΠΈΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΎΠ½Π½ΡΡ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ².
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