Структурно-функциональная характеристика гена рокристаллина лягушки Rana temporaria

5 ВЫВОДЫ.

1. Впервые получены доказательства хрусталикоспецифической экспрессии гена ро-кристаллина лягушки R. temporaria.

2. Сконструирована представительная кДНК библиотека на матрице мРНК из хрусталика лягушки R. temporaria. Идентифицирован клон, несущий полноразмерную последовательность кДНК р-кристаллина, и определена его первичная структура.

3. Создан геномный банк лягушки R. temporaria, из которого с помощью кДНК-зонда выделены клоны, содержащие участки гена р-кристаллина.

4. Построена детальная рестрикционная карта полученных геномных клонов.

5. Проведена структурная характеристика геномных клонов. Доказано наличие в геноме лягушки по крайней мере четырех копий гена р-кристаллина, один из которых вероятно является псевдогеном.

6. Впервые идентифицирован и структурно охарактеризован геномный клон, содержащий 5'концевой участок гена р-кристаллина, который по совокупности данных может содержать промотор гена р-кристаллина.

7. Компьютерный анализ структуры 5″ -участка гена р-кристаллина выявил гомологию с первичной структурой промоторного участка гена карбомил фосфат синтетазы-1 Rana catesbeiana, а также наличие ряда регуляторных элементов, характерных для промоторов других кристаллиновых генов.

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