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Исследование активности тобамовирусных участков внутренней инициации трансляции в различных растительных системах

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Tanaka Т., Nishihara M., Seki M., Sakamoro A., Tanaka K., Irifune K., Morikava H. 1995. Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment. // Plant Mol. Biol., 28, 337−341. Ali N. and Siddiqui A. 1995'. Interaction of polypyrimidine tract-binding protein with the 5'noncoding region of the hepatitis С virus RNA genome and its… Читать ещё >

Содержание

  • Список используемых сокращений
  • I. Введение
  • II. Обзор литературы
    • II. 1. Принципы инициации трансляции в растениях
    • 11. 2. Сканирующая модель инициации трансляции
  • II. 2.1. Кэп- и поли (А)-зависимая инициация трансляции. 13 И.2.2. Инициаторный кодон и его контекст
    • 11. 2. 2. 1. Механизм сканирования с пропусканием инициаторного код она
      • 11. 2. 2. 2. Терминация — реинициация
  • И.2.3. Влияние вторичной структуры
    • II. 2.3.1. Нелинейное сканирование (шунтирование)
  • 11. 3. Внутренняя инициация трансляции
    • 11. 3. 1. Фактор-зависимая внутренняя инициация трансляции
    • 11. 3. 2. Фактор-независимая внутренняя инициация трансляции
    • 11. 3. 3. Механизм комплементарного взаимодействия IRES и 18S рРНК
  • 11. 4. Внутренняя инициация трансляции в растениях
    • 11. 4. 1. Внутренняя инициации трансляции, обеспечиваемая последовательностями из генома тобамовирусов
    • 11. 4. 2. Внутренняя инициация трансляции в растениях -биологическое значение
  • III. Материалы и методы
  • IV. Результаты и обсуждение
    • IV. 1. Исследование активности IRES из РНК ВТМ U1 и кр
  • ВТМ в изолированных протопластах Nicotiana tabacum
  • IV. 1.1. Трансляция бицистронных конструкций в протопластах N. tabacum
  • IV. 1.2. Экспрессия в протопластах моноцистронных конструкций «Ш-IRES-GUS»
  • IV. 1.3. Исследование делеционных мутантов IRES
  • 1. 48бокр"в™ в растительных протопластах
    • IV. 2. Изучение активности IRES-последовательностей из генома кр-ВТМ в трансгенных растениях N. tabacum
  • IV. 2.1. ПЦР-анализ трансгенных растений
  • IV. 2.2. Анализ тотальной РНК выделенной из трансгенных растений
  • IV. 2.3. Исследование экспрессии первого гена и оценка эффективности работы различных IRES в растениях
    • IV. 3. Исследование влияния области внутренней посадки рибосом на инфекционность ВТМ
  • V. Выводы
  • Исследование активности тобамовирусных участков внутренней инициации трансляции в различных растительных системах (реферат, курсовая, диплом, контрольная)

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