ΠΠ΅ΠΏΡΠΈΠ΄Π½ΡΠ΅ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΡ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ
Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ Π΄ΠΈΠ·Π°ΠΉΠ½ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ Π·ΠΎΠ½Π΄ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΡ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΉΠΎΠ½Π° ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ. ΠΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ ΡΠΈΠ½ΡΠ΅Π· ΡΠ΅ΡΠΈΠΈ Π½ΠΎΠ²ΡΡ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΎΠ² 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΠΏΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π‘23 Ρ ΠΏΠ΅ΠΏΡΠΈΠ΄Π°ΠΌΠΈ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΌΠΈ ΠΎΡΡΠ°ΡΠΊΠΈ ΡΠ΅Π½ΠΈΠ»Π°Π»Π°Π½ΠΈΠ½Π° ΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- Π‘ΠΏΠΈΡΠΎΠΊ ΡΠΎΠΊΡΠ°ΡΠ΅Π½ΠΈΠΉ
- ΠΠ»Π°Π²Π° 1. Π Π°ΡΡΡΡΠΈΠΉ ΠΏΠ΅ΠΏΡΠΈΠ΄ Π² ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠΌ ΡΡΠ½Π½Π΅Π»Π΅ (ΠΠ±Π·ΠΎΡ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ)
- 1. 1. Π‘ΡΡΠΎΠ΅Π½ΠΈΠ΅ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΈΠ±ΠΎΡΠΎΠΌΡ
- 1. 1. 1. ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠΈΠ±ΠΎΡΠΎΠΌΡ
- 1. 1. 2. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΈΠ±ΠΎΡΠΎΠΌΡ
- 1. 2. Π ΠΈΠ±ΠΎΡΠΎΠΌΠ½ΡΠΉ ΡΡΠ½Π½Π΅Π»Ρ
- 1. 1. Π‘ΡΡΠΎΠ΅Π½ΠΈΠ΅ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΈΠ±ΠΎΡΠΎΠΌΡ
- Π‘Π°ΠΉΡ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Π½ΡΡ
Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ²
- 1. 3. ΠΠ·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ°ΡΡΡΡΠΈΡ
ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² ΡΠΎ ΡΡΠ΅Π½ΠΊΠ°ΠΌΠΈ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ
- 1. 3. 1. ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² cat ΠΈ clmA
- 1. 3. 2. Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½Π° secA
- 1. 3. 3. Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ Π±Π΅Π»ΠΊΠ° YbeL
- 1. 3. 4. Π’ΡΠΈΠΏΡΠΎΡΠ°Π½Π°Π·Π½ΡΠΉ ΠΎΠΏΠ΅ΡΠΎΠ½. ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ
- 1. 3. ΠΠ·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ°ΡΡΡΡΠΈΡ
ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² ΡΠΎ ΡΡΠ΅Π½ΠΊΠ°ΠΌΠΈ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ
- 2. 1. ΠΠΈΠ·Π°ΠΉΠ½ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΠΠ’ ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠΎΠ² Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π Π’
- 2. 2. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠ° 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π°
- 2. 3. Π Π°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΠΌΠ΅ΡΠΎΠ΄Π° ΡΠΈΠ½ΡΠ΅Π·Π° Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΠΠ’ ΠΏΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π‘
- 2. 4. Π‘ΠΈΠ½ΡΠ΅Π· N-Π·Π°ΡΠΈΡΠ΅Π½Π½ΡΡ ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΎΡΡΠ°ΡΠΊΠΈ ΡΠ΅Π½ΠΈΠ»Π°Π»Π°Π½ΠΈΠ½Π° ΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°
- 2. 5. Π‘ΠΈΠ½ΡΠ΅Π· ΡΠ΅Π½ΠΈΠ»Π°Π»Π°Π½ΠΈΠ½- ΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΠΠ’ ΠΏΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π‘
- 2. 6. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ
ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
ΠΠΠ’ ΠΏΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ
- 2. 6. 1. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ ΠΠΠ’ ΠΈ Π΅Π³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ Ρ ΡΠΈΠ±ΠΎΡΠΎΠΌΠΎΠΉ
- 2. 6. 2. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅ΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΠΠ’ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ-ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° GFP
- 2. 6. 3. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅ΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΠΠ’ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ ΠΌΠ ΠΠ Π»ΡΡΠΈΡΠ΅ΡΠ°Π·Ρ ΡΠ²Π΅ΡΠ»ΡΡΠΊΠΎΠ²
- 2. 6. 4. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΠΠ’
- 3. 1. ΠΡΡ
ΠΎΠ΄Π½ΡΠ΅ Π²Π΅ΡΠ΅ΡΡΠ²Π° ΠΈ ΠΎΡΠΈΡΡΠΊΠ° ΡΠ°ΡΡΠ²ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ
- 3. 1. 1. ΠΡΡ ΠΎΠ΄Π½ΡΠ΅ Π²Π΅ΡΠ΅ΡΡΠ²Π°
- 3. 1. 2. ΠΠ±ΡΠΎΠ»ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ°ΡΡΠ²ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ
- 3. 2. ΠΡΠΏΠΎΠΌΠΎΠ³Π°ΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ
- 3. 2. 1. Π₯ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡ Π² ΡΠΎΠ½ΠΊΠΎΠΌ ΡΠ»ΠΎΠ΅
- 3. 2. 2. ΠΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½Π°Ρ ΡΠΎΠ½ΠΊΠΎΡΠ»ΠΎΠΉΠ½Π°Ρ Ρ ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡ
- 3. 2. 3. ΠΠΎΠ»ΠΎΠ½ΠΎΡΠ½Π°Ρ Ρ ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡ
- 3. 2. 4. ΠΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½Π°Ρ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½Π°Ρ Ρ ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡ
- 3. 2. 5. ΠΠΈΠ΄ΡΠΎΠ»ΠΈΠ· ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² ΠΈ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ·
- 3. 2. 6. ΠΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡ
- 3. 2. 7. Π―ΠΠ -ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡ
- 3. 3. ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π²Π΅ΡΠ΅ΡΡΠ²
- 3. 4. ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΡΠΏΡΡΠ°Π½ΠΈΠΉ
- 3. 4. 1. ΠΡΡΠ΅ΡΡ ΠΈ ΡΠ°ΡΡΠ²ΠΎΡΡ
- 3. 4. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΈΠ±ΠΎΡΠΎΠΌ Π. col
- 3. 4. 3. Π₯ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΡΠΎΠ±ΠΈΠ½Π³
- 3. 4. 4. ΠΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ΅ Π²ΡΡΠ΅ΡΠ½Π΅Π½ΠΈΠ΅ ΡΠ°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎ ΠΌΠ΅ΡΠ΅Π½ΠΎΠ³ΠΎ [14Π‘]-ΡΡΠΈΡΡΠΎΠΌΠΈΡΠΈΠ½Π°
- 3. 4. 5. Π‘ΠΈΡΡΠ΅ΠΌΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ-ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° GFP
- 3. 4. 6. Π‘ΠΈΡΡΠ΅ΠΌΠ° ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ ΠΌΠ ΠΠ Π»ΡΡΠΈΡΠ΅ΡΠ°Π·Ρ ΡΠ²Π΅ΡΠ»ΡΡΠΊΠΎΠ²
ΠΠ΅ΠΏΡΠΈΠ΄Π½ΡΠ΅ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΡ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
Π ΠΈΠ±ΠΎΡΠΎΠΌΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΠΌΠ°ΡΠΈΠ½Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΡΡΡ Π²ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΠΆΠΈΠ²ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠΈ, ΡΡΠ°Π½ΡΠ»ΠΈΡΡΡ Ρ ΡΠ°Π½ΡΡΡΡΡΡ Π² Π³Π΅Π½ΠΎΠΌΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΡΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡ. Π Ρ ΠΎΠ΄Π΅ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΡΠ΅ΠΌΠ°Ρ Π½Π° ΡΠΈΠ±ΠΎΡΠΎΠΌΠ΅ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½Π°Ρ ΡΠ΅ΠΏΡ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΠΏΠ΅ΡΠ΅ΠΌΠ΅ΡΠ°Π΅ΡΡΡ ΠΏΠΎ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠΌΡ ΡΡΠ½Π½Π΅Π»Ρ (Π Π’) ΠΎΡ ΠΌΠ΅ΡΡΠ° ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΠ΅ΡΠ΅Π΄Π½ΠΎΠΉ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΠΎΠΉ ΡΠ²ΡΠ·ΠΈ (ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΈΠ»ΡΡΠ°Π½ΡΡΠ΅ΡΠ°Π·Π½ΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ°, ΠΠ’Π¦) ΠΊ ΠΌΠ΅ΡΡΡ Π²ΡΡ ΠΎΠ΄Π° ΠΈΠ· ΡΠΈΠ±ΠΎΡΠΎΠΌΡ, Π³Π΄Π΅ Π½Π°ΡΠΈΠ½Π°Π΅ΡΡΡ ΠΏΡΠΎΡΠ΅ΡΡ «ΡΠΎΠ»Π΄ΠΈΠ½Π³Π°» Π±Π΅Π»ΠΊΠ°. Π Π’ ΠΈΠ·ΡΡΠ°Π΅ΡΡΡ ΡΠΆΠ΅ ΠΏΠΎΡΡΠΈ Π΄Π΅ΡΡΡΡ Π»Π΅Ρ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·Π½ΡΡ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈΡ , Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ². Π ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ, ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π΅Π³ΠΎ ΡΡΠ΅Π½ΠΊΠΈ «Π²ΡΠ»ΠΎΠΆΠ΅Π½Ρ» ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠΌΠΈ ΠΎΡΡΠ°ΡΠΊΠ°ΠΌΠΈ ΡΠ ΠΠ. Π Ρ ΠΎΠ΄Π΅ ΡΠΈΠ½ΡΠ΅Π·Π° Π±Π΅Π»ΠΊΠ° Π² Π Π’ Π½Π°Ρ ΠΎΠ΄ΠΈΡΡΡ ΡΠ΅Π³ΠΌΠ΅Π½Ρ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π° Π΄Π»ΠΈΠ½ΠΎΠΉ 25−30 Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΎΡΡΠ°ΡΠΊΠΎΠ² [1,2]. ΠΠ·Π²Π΅ΡΡΠ½ΠΎ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΎ ΠΎΡΠΎΠ±ΡΡ ΡΠ»ΡΡΠ°Π΅Π², ΠΊΠΎΠ³Π΄Π° Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΠΎΡΡΠ°ΡΠΊΠ° Π²Π½ΠΎΠ²Ρ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΡΠ΅ΠΌΠΎΠ³ΠΎ ΠΏΠ΅ΠΏΡΠΈΠ΄Π° ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΡΠ°ΡΡΠΊΠΎΠΌ Π Π’ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠ΅ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΡΠΈΠ½ΡΠ΅Π·Π°. ΠΡΠΎ ΡΠ²Π»Π΅Π½ΠΈΠ΅ Π»Π΅ΠΆΠΈΡ Π² ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠ΄Π° Π³Π΅Π½ΠΎΠ² Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ [3]. Π ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ, ΡΠ°ΠΊΠΈΠ΅ ΠΊΠ»ΡΡΠ΅Π²ΡΠ΅ Π²ΠΎΠΏΡΠΎΡΡ, ΠΊΠ°ΠΊ ΠΊΠΎΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΠΏΠΈ Π² Π Π’, ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ Π΅Π΅ ΠΏΠ΅ΡΠ΅ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎ ΡΡΠ½Π½Π΅Π»Ρ ΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Ρ ΡΠ ΠΠ ΠΎΡΡΠ°ΡΡΡΡ Π±Π΅Π· ΠΎΡΠ²Π΅ΡΠ°. ΠΠ΅Π΄Π°Π²Π½ΠΎ Π² Π½Π°ΡΠ΅ΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π±ΡΠ»ΠΎ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ΠΎ ΠΏΠΎΠΏΡΡΠ°ΡΡΡΡ Π΄Π°ΡΡ ΠΎΡΠ²Π΅Ρ Π½Π° ΡΡΠΈ Π²ΠΎΠΏΡΠΎΡΡ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Π½ΡΡ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ² [4].
ΠΠ΅ΠΉΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎ, ΠΎΠ΄Π½Π° ΠΈΠ· ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ Π Π’ ΡΠΎΡΡΠΎΠΈΡ Π² ΡΠΎΠΌ, ΡΡΠΎ Π²Π±Π»ΠΈΠ·ΠΈ ΠΠ’Π¦ Π² Π½Π΅ΠΌ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ ΡΠ°ΠΉΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ²-ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄ΠΎΠ². ΠΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²ΠΎ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΡ ΠΈ ΠΏΠΎΠ»ΡΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΎΠ΅ ΠΌΠ°ΠΊΡΠΎΡΠΈΠΊΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π»Π°ΠΊΡΠΎΠ½Π½ΠΎΠ΅ ΠΊΠΎΠ»ΡΡΠΎ, Π·Π°ΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠ΅ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ»ΠΈ Π±ΠΎΠ»Π΅Π΅ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΡΠΌΠΈ ΠΎΡΡΠ°ΡΠΊΠ°ΠΌΠΈ. Π ΡΡΠΎΠΌΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Ρ ΠΎΡΠ½ΠΎΡΡΡΡΡ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΈ ΡΠΈΠ»ΠΎΠ·ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ΄Π°, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ 16-ΡΠ»Π΅Π½Π½ΡΠΉ Π»Π°ΠΊΡΠΎΠ½Π½ΡΠΉ ΡΠΈΠΊΠ» — ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄. ΠΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Ρ ΡΠΈΡΠΎΠΊΠΎ ΠΏΡΠΈΠΌΠ΅Π½ΡΡΡΡΡ Π½Π° ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, Π²ΡΠ·ΡΠ²Π°Π΅ΠΌΡΡ Π³ΡΠ°ΠΌΠΌ-ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΡΠΌΠΈ. Π€Π°ΡΠΌΠ°ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄ΠΎΠ² ΠΎΡΠ½ΠΎΠ²ΡΠ²Π°Π΅ΡΡΡ Π½Π° ΠΈΡ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠΈ Ρ ΡΠΈΠ±ΠΎΡΠΎΠΌΠΎΠΉ ΠΈ Π±Π»ΠΎΠΊΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΠ΅ΡΠ΅ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΠΏΠΈ Π±Π΅Π»ΠΊΠ° Π² ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠΌ ΡΡΠ½Π½Π΅Π»Π΅. ΠΠΎΡΡΠΎΠΌΡ ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Π½ΡΠ΅ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΈ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ½Ρ ΠΊΠ°ΠΊ ΡΠ΄ΠΎΠ±Π½ΡΠ΅ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΡ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ. Π‘ Π΄ΡΡΠ³ΠΎΠΉ ΡΡΠΎΡΠΎΠ½Ρ, ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΡΡ ΡΡΠ±ΡΠ΅Π΄ΠΈΠ½ΠΈΡ Ρ ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Π°ΠΌΠΈ (Π° ΡΠ°ΠΊΠΆΠ΅ Ρ ΠΌΠ½ΠΎΠ³ΠΎΡΠΈΡΠ»Π΅Π½Π½ΡΠΌΠΈ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠ°ΠΌΠΈ Π΄ΡΡΠ³ΠΈΡ ΠΊΠ»Π°ΡΡΠΎΠ²) Π²Π°ΠΆΠ½ΠΎ Π΄Π»Ρ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, Π΄Π»Ρ Π²ΡΡΡΠ½Π΅Π½ΠΈΡ ΠΏΡΠΈΡΠΈΠ½ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΠΈ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΊ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠ°ΠΌ ΠΈ Π΄Π»Ρ ΡΠ°ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΡΠΈΡ Π·Π½Π°Π½ΠΈΠΉ Π½ΠΎΠ²ΡΡ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΡΠ΅Π΄ΡΡΠ².
Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ΅ΡΡΡ Π΄ΠΈΠ·Π°ΠΉΠ½, ΡΠΈΠ½ΡΠ΅Π· ΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠ°-ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Π° ΡΠΈΠ»ΠΎΠ·ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ΄Π° 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° (ΠΠΠ’) Ρ ΡΠ΅Π»ΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠ° Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ.
ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΠΉ ΠΎΠ±Π·ΠΎΡ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ°ΡΡΡΡΠ΅Π³ΠΎ ΠΏΠ΅ΠΏΡΠΈΠ΄Π° Π² Π Π’ ΠΈ Π΅Π³ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ ΡΠΎ ΡΡΠ΅Π½ΠΊΠ°ΠΌΠΈ ΡΡΠ½Π½Π΅Π»Ρ.
ΠΡΠ²ΠΎΠ΄Ρ.
1. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ Π΄ΠΈΠ·Π°ΠΉΠ½ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ Π·ΠΎΠ½Π΄ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΡ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΉΠΎΠ½Π° ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ.
2. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄ ΡΠΈΠ½ΡΠ΅Π·Π° Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ ΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ ΠΏΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π‘23 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π°.
3. ΠΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ ΡΠΈΠ½ΡΠ΅Π· ΡΠ΅ΡΠΈΠΈ Π½ΠΎΠ²ΡΡ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΎΠ² 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΠΏΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π‘23 Ρ ΠΏΠ΅ΠΏΡΠΈΠ΄Π°ΠΌΠΈ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΌΠΈ ΠΎΡΡΠ°ΡΠΊΠΈ ΡΠ΅Π½ΠΈΠ»Π°Π»Π°Π½ΠΈΠ½Π° ΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°.
4. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π½ΠΈΠ»Π°Π»Π°Π½ΠΈΠ½ΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ 5-Π-ΠΌΠΈΠΊΠ°ΠΌΠΈΠ½ΠΎΠ·ΠΈΠ»ΡΠΈΠ»ΠΎΠ½ΠΎΠ»ΠΈΠ΄Π° ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΡΠΌ ΡΡΠ½Π½Π΅Π»Π΅ΠΌ Π² ΠΌΠ°ΠΊΡΠΎΠ»ΠΈΠ΄Π½ΠΎΠΌ ΡΠ°ΠΉΡΠ΅ ΠΈ, ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΡ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΡΠ½Π½Π΅Π»Ρ.
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