Π‘ΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ ΠΈΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π°Π·Ρ β Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΈΠ· Π΄ΡΠΎΠ΄Π΅Π½ΡΠΌΠ° Π±ΡΠΊΠ°
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅Π΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π° ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ ΠΏΡΠΎΡΠ΅Π°Π·Ρ ΡΡΠ΄Π° ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΡ ΠΏΡΠΎΡΠ΅Π°Π·. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ, ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎ Ρ ΠΈΠΌΠ°Π·Π°ΠΌ, ΡΠ΅ΡΠΌΠ΅Π½Ρ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΠ΅ΡΡΡ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ°ΠΌΠΈ ΡΠΈΠΏΠ° ΠΡΠ½ΠΈΡΡΠ° ΠΈ ΠΠ°ΡΠΌΠ°Π½Π°-ΠΠΈΡΠΊ ΠΈΠ· Π±ΠΎΠ±ΠΎΠ²ΡΡ , Π½ΠΎ ΠΏΡΠΎΡΠ²Π»ΡΠ΅Ρ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡ ΠΊ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠΈΠΏΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ° BPTI. ΠΠ· ΡΠ»ΠΈΠ·ΠΈΡΡΠΎΠΉ Π΄ΡΠΎΠ΄Π΅Π½ΡΠΌΠ° Π±ΡΠΊΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΡΡΠΈΠ½Π½ΠΎΠΉ… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- I. Π‘Π ΠΠΠΠΠ’ΠΠΠ¬ΠΠΠ― Π₯ΠΠ ΠΠΠ’ΠΠ ΠΠ‘Π’ΠΠΠ ΠΠ£ΠΠΠΠΠΠΠ« Π Π₯ΠΠΠΠ, Π€ΠΠ ΠΠΠΠ’ΠΠ, Π‘Π’Π Π£ΠΠ’Π£Π ΠΠ Π ΠΠΠ‘Π’ΠΠΠΠΠ«Π₯ Π₯ΠΠΠΠΠΠΠΠΠΠΠΠΠ ΠΠ ΠΠ’ΠΠΠΠ (Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΠΉ ΠΎΠ±Π·ΠΎΡ)
- 1. ΠΡΠΎΠ΄Π΅Π½Π°Π·Π°, ΡΠ΅ΡΠΈΠ½ΠΎΠ²Π°Ρ ΠΏΡΠΎΡΠ΅Π°Π·Π° ΡΠΏΠΈΡΠ΅Π»ΠΈΠΎΡΠΈΡΠΎΠ² Π±ΡΡΠ½Π½Π΅ΡΠΎΠ²ΡΡ
ΠΆΠ΅Π»Π΅Π·
- 1. 1. ΠΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΈ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΠ°Ρ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΎΠ»Ρ Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Ρ
- 1. 2. ΠΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈ ΡΡΡΡΠΊΡΡΡΠ° Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Ρ
- 1. 3. ΠΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Ρ
- 2. Π₯ΠΈΠΌΠ°Π·Ρ ΡΡΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 1. ΠΠ΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΡΡΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 2. Π’ΠΊΠ°Π½Π΅Π²Π°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Ρ ΠΈΠΌΠ°Π·
- 2. 3. Π‘ΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ Ρ ΠΈΠΌΠ°Π·
- 2. 4. Π£ΠΏΠ°ΠΊΠΎΠ²ΠΊΠ° ΠΈ Ρ ΡΠ°Π½Π΅Π½ΠΈΠ΅ Ρ ΠΈΠΌΠ°Π· Π² ΡΠ΅ΠΊΡΠ΅ΡΠΎΡΠ½ΡΡ Π³ΡΠ°Π½ΡΠ»Π°Ρ
- 2. 5. ΠΠΊΡΠΈΠ²Π°ΡΠΈΡ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² Ρ ΠΈΠΌΠ°Π·
- 2. 6. Π‘ΡΠ±ΡΡΡΠ°ΡΠ½Π°Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ Ρ
ΠΈΠΌΠ°Π·
- 2. 6. 1. Π‘ΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ
- 2. 6. 2. ΠΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΠ΅ ΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ
- 2. 7. ΠΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΡ Ρ
ΠΈΠΌΠ°Π·
- 2. 7. 1. Π‘ΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΡ
- 2. 7. 2. ΠΡΠΈΡΠΎΠ΄Π½ΡΠ΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΡ Ρ ΠΈΠΌΠ°Π·
- 2. 8. Π€ΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΎΠ»Ρ Ρ ΠΈΠΌΠ°Π·
- II. Π ΠΠΠ£ΠΠ¬Π’ΠΠ’Π« Π ΠΠΠ‘Π£ΠΠΠΠΠΠ
- 1. ΠΡΠΈΡΡΠΊΠ° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 2. ΠΡΠΈΡΠ΅ΡΠΈΠΈ ΡΠΈΡΡΠΎΡΡ ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 3. Π‘ΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 4. N-ΠΊΠΎΠ½ΡΠ΅Π²Π°Ρ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ChIP
- 5. ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΠ, ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ ΠΈ ΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΈΠ»Ρ Π½Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 6. Π‘ΡΠ±ΡΡΡΠ°ΡΠ½Π°Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ChIP
- 6. 1. Π‘ΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ
- 6. 2. ΠΡΠΈΡΠΎΠ΄Π½ΡΠ΅ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ
- 7. ΠΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ·
- 8. ΠΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΈΡΡΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ChIP
- III. ΠΠΠ‘ΠΠΠ ΠΠΠΠΠ’ΠΠΠ¬ΠΠΠ― Π§ΠΠ‘Π’
- 1. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ
- 2. ΠΠ΅ΡΠΎΠ΄Ρ
- 2. 1. ΠΡΠΈΡΡΠΊΠ° Ρ ΠΈΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ Π»ΡΠΎΡΠ΅Π°Π·Ρ
- 2. 2. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠ°
- 2. 3. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ
- 2. 4. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 2. 5. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ²
- 2. 6. ΠΠΈΠ΄ΡΠΎΠ»ΠΈΠ· Π±Π΅Π»ΠΊΠΎΠ² ΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² ChIP
- 2. 7. ΠΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ·
- 2. 8. ΠΠ΅Π»Ρ-ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·
- 2. 9. ΠΠ½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ChIP
- 2. 10. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ-Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ChIP
- 2. 11. ΠΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ChIP
- 2. 12. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ N-ΠΊΠΎΠ½ΡΠ΅Π²ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π±Π΅Π»ΠΊΠ°
- 2. 13. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΡΠ΅Π» ΠΊ ChIP
- 2. 14. ΠΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ·
- ΠΠ«ΠΠΠΠ«
Π‘ΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ ΠΈΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π°Π·Ρ β Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΈΠ· Π΄ΡΠΎΠ΄Π΅Π½ΡΠΌΠ° Π±ΡΠΊΠ° (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ (ΠΏΡΠΎΡΠ΅Π°Π·Ρ), Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΏΡΠΎΡΠ΅Π°Π·Ρ, ΠΈΠ³ΡΠ°ΡΡ Π²Π°ΠΆΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΎΠΎΠ±ΡΠ°Π·Π½ΡΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π²ΡΠ΅Ρ ΠΆΠΈΠ²ΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΡ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² ΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² ΠΈ ΡΠ΅ΠΌ ΡΠ°ΠΌΡΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡ Π½Π°ΡΠ°Π»ΠΎ ΠΈΠ»ΠΈ ΠΏΡΠ΅ΠΊΡΠ°ΡΠ΅Π½ΠΈΠ΅ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ².
Π‘Π΅ΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΏΡΠΎΡΠ΅Π°Π·Ρ ΡΠΈΡΠΎΠΊΠΎ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Ρ Π² ΠΏΡΠΈΡΠΎΠ΄Π΅ ΠΈ ΠΏΡΠΈΡΡΡΡΡΠ²ΡΡΡ ΠΊΠ°ΠΊ Π² ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°Ρ , ΡΠ°ΠΊ ΠΈ Π² Π²ΡΡΡΠΈΡ ΡΠ°ΡΡΠ΅Π½ΠΈΡΡ ΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ , Π²ΠΊΠ»ΡΡΠ°Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°.
ΠΡΠ΅ ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΏΡΠΎΡΠ΅Π°Π·Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ Π΄Π°Π½Π½ΡΡ ΠΎΠ± ΠΈΡ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΠΎΠΌ ΡΡΡΠΎΠ΅Π½ΠΈΠΈ ΡΠ°Π·Π΄Π΅Π»ΡΡΡ Π½Π° ΡΡΠΈ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°: Ρ ΠΈΠΌΠΎΡΡΠΈΠΏΡΠΈΠ½Π°, ΡΡΠ±ΡΠΈΠ»ΠΈΠ·ΠΈΠ½Π° ΠΈ ΠΊΠ°ΡΠ±ΠΎΠΊΡΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Ρ II. ΠΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»ΠΈ ΡΡΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ² Π±Π΅ΡΡΡ Π½Π°ΡΠ°Π»ΠΎ ΠΎΡ ΡΠ°Π·Π½ΡΡ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΡΡ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² ΠΈ ΠΎΡΠ»ΠΈΡΠ°ΡΡΡΡ Π΄ΡΡΠ³ ΠΎΡ Π΄ΡΡΠ³Π° ΠΏΠΎ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΡΡΡΡΠΊΡΡΡΠ΅ ΠΈ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΊΠ»Π°Π΄ΠΊΠ΅ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΡΠ΅ΠΏΠ΅ΠΉ. ΠΠ΄Π½Π°ΠΊΠΎ, Π²ΡΠ΅ ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΏΡΠΎΡΠ΅Π°Π·Ρ ΡΠ°Π·Π½ΡΠΌΠΈ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΡΠΌΠΈ ΠΏΡΡΡΠΌΠΈ ΠΏΡΠΈΡΠ»ΠΈ ΠΊ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΠΎΠΌΡ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ Π² Π°ΠΊΡΠΈΠ²Π½ΠΎΠΌ ΡΠ΅Π½ΡΡΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΎΡΡΠ°ΡΠΊΠΈ His, Ser ΠΈ Asp Π² ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½ΠΎΠΉ Π³Π΅ΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΎΡΠΈΠ΅Π½ΡΠ°ΡΠΈΠΈ.
Π‘Π΅ΠΌΠ΅ΠΉΡΡΠ²ΠΎ Ρ ΠΈΠΌΠΎΡΡΠΈΠΏΡΠΈΠ½Π° Π²ΠΊΠ»ΡΡΠ°Π΅Ρ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΡΠΈΡΠ»ΠΎ ΠΏΡΠΎΡΠ΅Π°Π· ΠΈΠ· Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ ΠΈ Π²ΡΡΡΠΈΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΈ ΠΏΡΠΈΠ²Π»Π΅ΠΊΠ°Π΅Ρ Π±ΠΎΠ»ΡΡΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ. Π ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ΅ ΠΎΠ½ΠΈ Π²ΡΠΏΠΎΠ»Π½ΡΡΡ ΠΊΠ°ΠΊ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΡ, ΡΠ°ΠΊ ΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΡΠ½ΠΊΡΠΈΠΈ: Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ· Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΏΠΈΡΠΈ (ΡΠ΅ΡΠΌΠ΅Π½ΡΡ ΠΏΠΎΠ΄ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΡΠΈΠΏΡΠΈΠ½, Ρ ΠΈΠΌΠΎΡΡΠΈΠΏΡΠΈΠ½, ΡΠ»Π°ΡΡΠ°Π·Π°,) — ΠΏΠΎΡΡΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΎΠ½Π½ΡΠΉ ΠΏΡΠΎΡΠ΅ΡΡΠΈΠ½Π³ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ², Π³ΠΎΡΠΌΠΎΠ½ΠΎΠ², ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (ΡΠ½ΡΠ΅ΡΠΎΠΏΠ΅ΠΏΡΠΈΠ΄Π°Π·Π°, ΠΊΠ°Π»Π»ΠΈΠΊΡΠ΅ΠΈΠ½, ΡΡΠΈΠΏΡΠΈΠ½, Π³ΡΠ°Π½Π·ΠΈΠΌ Π) — ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΊΠ°ΡΠΊΠ°Π΄Π½ΡΡ ΡΠ΅Π°ΠΊΡΠΈΡΡ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ Π·ΠΈΠΌΠΎΠ³Π΅Π½ΠΎΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ², ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ²Π΅ΡΡΡΠ²Π°Π½ΠΈΡ ΠΊΡΠΎΠ²ΠΈ ΠΈ ΡΠΈΠ±ΡΠΈΠ½ΠΎΠ»ΠΈΠ·Π° (ΠΏΠ»Π°Π·ΠΌΠΈΠ½, ΡΡΠΎΠΌΠ±ΠΈΠ½, ΡΠ°ΠΊΡΠΎΡΡ Va, Vila, Villa, 1Π₯Π°, Π₯Π° ΠΈ Π΄Ρ.) ΠΈ ΡΠΈΡΡΠ΅ΠΌΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ° (ΡΠ°ΠΊΡΠΎΡΡ Π‘1Π³, C1s, I, D). ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΏΡΠΎΡΠ΅Π°Π·Ρ ΡΡΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΈΠ³ΡΠ°ΡΡ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΡΠΎΠ»Ρ Π² ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°ΡΠΈΠΈ ΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ.
ΠΠ»Ρ ΠΌΠ½ΠΎΠ³ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΊ Π½Π°ΡΡΠΎΡΡΠ΅ΠΌΡ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ Ρ ΠΎΡΠΎΡΠΎ ΠΈΠ·Π²Π΅ΡΡΠ½Ρ ΡΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈ Π²ΡΡΠ²Π»Π΅Π½Ρ ΠΈΡ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ. ΠΠ΄Π½Π°ΠΊΠΎ, ΡΡΡΠ΅ΡΡΠ²ΡΠ΅Ρ ΡΡΠ΄ ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΡ ΠΏΡΠΎΡΠ΅Π°Π· ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° Ρ ΠΈΠΌΠΎΡΡΠΈΠΏΡΠΈΠ½Π°, ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ Π½Π°ΡΠ°Π»ΠΎΡΡ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎ Π½Π΅Π΄Π°Π²Π½ΠΎ. Π ΡΠΈΡΠ»Ρ ΡΠ°ΠΊΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΎΡΠ½ΠΎΡΡΡΡΡ ΠΏΡΠΎΡΠ΅Π°Π·Ρ ΡΠ΅ΠΊΡΠ΅ΡΠΎΡΠ½ΡΡ Π³ΡΠ°Π½ΡΠ» Π³Π΅ΠΌΠΎΠΏΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ , ΠΎΠ±ΡΠ΅Π΄ΠΈΠ½Π΅Π½Π½ΡΡ Π² Π³ΡΡΠΏΠΏΡ ΡΠ°ΠΊ Π½Π°Π·ΡΠ²Π°Π΅ΠΌΡΡ Π³ΡΠ°Π½Π·ΠΈΠΌΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ ΠΏΡΠΎΡΠ΅Π°Π· — Ρ ΠΈΠΌΠ°Π·Ρ ΡΡΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½ G Π½Π΅ΠΉΡΡΠΎΡΠΈΠ»ΠΎΠ², Π³ΡΠ°Π½Π·ΠΈΠΌΡ ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΈ Π½Π°ΡΡΡΠ°Π»ΡΠ½ΡΡ ΠΊΠΈΠ»Π»Π΅ΡΠΎΠ². ΠΠΎ ΡΠΈΡ ΠΏΠΎΡ ΠΈΡ ΡΠΎΡΠ½Π°Ρ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΎΠ»Ρ Π½Π΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π°, Π½ΠΎ ΠΌΠ½ΠΎΠ³ΠΎΡΠΈΡΠ»Π΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ ΠΎΠ½ΠΈ ΠΈΠ³ΡΠ°ΡΡ Π²Π°ΠΆΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΠΈΠΌΠΌΡΠ½Π½ΠΎΠΌ ΠΎΡΠ²Π΅ΡΠ΅ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°, ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΠΈ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·Π°, ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΡΡΠ΄Π° ΠΏΠ°ΡΠΎΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ². Π’Π°ΠΊ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ, Π΄Π»Ρ Π³ΡΠ°Π½Π·ΠΈΠΌΠΎΠ², Π ΠΈ Π ΠΈΠ· ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠ½ΠΈ ΡΡΠ°ΡΡΠ²ΡΡΡ Π² Π·Π°ΠΏΡΡΠΊΠ΅ Π°ΠΏΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΊΠ»Π΅ΡΠΎΠΊ-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ, ΠΈΠ½ΠΈΡΠΈΠΈΡΡΡ ΠΊΠ°ΡΠΊΠ°Π΄ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΠΊΠ°ΡΠΏΠ°Π·, Π²Π°ΠΆΠ½ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π°ΠΏΠΎΠΏΡΠΎΠ·Π°. ΠΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½Π° G ΡΠ²ΡΠ·Π°Π½Π° Ρ ΡΠ°Π³ΠΎΡΠΈΡΠΎΠ·ΠΎΠΌ — ΡΡΠ½ΠΊΡΠΈΠ΅ΠΉ Π°Π·ΡΡΠΎΡΠΈΠ»ΡΠ½ΡΡ Π³ΡΠ°Π½ΡΠ», Π² ΠΊΠΎΡΠΎΡΡΡ Ρ ΡΠ°Π½ΠΈΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½Ρ, -. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, ΠΎΠ½ ΡΠΏΠΎΡΠΎΠ±Π΅Π½ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ Π°ΠΊΡΠΈΠ²Π½ΡΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ².
ΠΠ½ΡΠ΅ΡΠ΅Ρ ΠΊ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π³ΡΠ°Π½Π·ΠΈΠΌΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ ΠΏΡΠΎΡΠ΅Π°Π· ΠΊΠ»Π΅ΡΠΎΠΊ Π³Π΅ΠΌΠΎΠΏΠΎΡΠ·Π° Π½Π΅ ΠΎΡΠ»Π°Π±Π΅Π²Π°Π΅Ρ, ΡΡΠΎ ΠΎΠ±ΡΡΠ»Π°Π²Π»ΠΈΠ²Π°Π΅ΡΡΡ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΡΠΎΠΉ Π³ΡΡΠΏΠΏΡ Π² ΠΆΠΈΠ·Π½Π΅Π½Π½ΠΎ Π²Π°ΠΆΠ½ΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°. Π§ΠΈΡΠ»ΠΎ Π³ΡΠ°Π½Π·ΠΈΠΌΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ ΠΏΡΠΎΡΠ΅Π°Π· ΠΏΠΎΡΡΠΎΡΠ½Π½ΠΎ ΠΏΠΎΠΏΠΎΠ»Π½ΡΠ΅ΡΡΡ Π½ΠΎΠ²ΡΠΌΠΈ, Π½Π΅ΠΈΠ·Π²Π΅ΡΡΠ½ΡΠΌΠΈ ΡΠ°Π½Π΅Π΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»ΡΠΌΠΈ.
ΠΠΎΠ»Π΅Π΅ ΡΠΎΠ³ΠΎ, ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ΅ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠΈΡΠ΅ ΠΈ Π½Π΅ ΠΎΠ³ΡΠ°Π½ΠΈΡΠΈΠ²Π°ΡΡΡΡ ΡΠΎΠ»ΡΠΊΠΎ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ, ΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎ, ΡΠ°ΡΡΠΈΡΡΠ΅ΡΡΡ ΠΈ ΡΠΏΠ΅ΠΊΡΡ ΠΈΡ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ.
ΠΠ°ΡΡΠΎΡΡΠ°Ρ ΡΠ°Π±ΠΎΡΠ° ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π½ΠΎΠ²ΠΎΠΉ ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π°Π·Ρ, ΡΡΡΡΠΊΡΡΡΠ½ΠΎ Π±Π»ΠΈΠ·ΠΊΠΎΠΉ Π³ΡΠ°Π½Π·ΠΈΠΌΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΡΠΌ ΠΏΡΠΎΡΠ΅Π°Π·Π°ΠΌ (Ρ ΠΈΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π°Π·Ρ), ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π½ΠΎΠΉ Π² ΡΠ»ΠΈΠ·ΠΈΡΡΠΎΠΉ Π΄ΡΠΎΠ΄Π΅Π½ΡΠΌΠ° ΠΊΡΡΠΏΠ½ΠΎΠ³ΠΎ ΡΠΎΠ³Π°ΡΠΎΠ³ΠΎ ΡΠΊΠΎΡΠ° Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠΈΡΡΠ΅ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΡΠΎΠ½ΠΊΠΎΠ³ΠΎ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ° ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ , ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΠΎΠ³ΠΎ Π² Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Ρ ΠΈΠΌΠΈΠΈ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΠΠ₯. Π¦Π΅Π»Ρ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΠΎΡΠΈΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°, ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π΅Π³ΠΎ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈ ΡΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ²ΠΎΠΉΡΡΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°.
I. Π‘Π ΠΠΠΠΠ’ΠΠΠ¬ΠΠΠ― Π₯ΠΠ ΠΠΠ’ΠΠ ΠΠ‘Π’ΠΠΠ ΠΠ£ΠΠΠΠΠΠΠ« Π Π₯ΠΠΠΠ,.
Π€ΠΠ ΠΠΠΠ’ΠΠ, Π‘Π’Π Π£ΠΠ’Π£Π ΠΠ Π ΠΠΠ‘Π’ΠΠΠΠΠ«Π₯ Π₯ΠΠΠΠΠΠΠΠΠΠΠΠΠ ΠΠ ΠΠ’ΠΠΠΠ (Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΠΉ ΠΎΠ±Π·ΠΎΡ).
ΠΠ°ΡΡΠΎΡΡΠΈΠΉ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΠΉ ΠΎΠ±Π·ΠΎΡ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌ Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Ρ ΠΈ Ρ ΠΈΠΌΠ°Π· — ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΠΌ ΠΏΡΠΎΡΠ΅Π°Π·Π°ΠΌ, ΡΡΡΡΠΊΡΡΡΠ½ΠΎ ΠΈ ΡΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠΎΠ΄ΡΡΠ²Π΅Π½Π½ΡΠΌ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΌΡ Π½Π°ΠΌΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ Ρ ΠΈΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π°Π·Π΅ (ChIP). Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½ΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ² Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Ρ (ΠΏΠ΅ΡΠ²ΡΠΉ ΡΠ°Π·Π΄Π΅Π») ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ½ΡΠΌ Π² ΡΠ²ΡΠ·ΠΈ Ρ ΡΠ΅ΠΌ, ΡΡΠΎ ChIP ΠΈ Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Π° Π±ΡΠ»ΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½Ρ ΠΈΠ· ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠ° (ΡΠ»ΠΈΠ·ΠΈΡΡΠ°Ρ Π΄ΡΠΎΠ΄Π΅Π½ΡΠΌΠ° Π±ΡΠΊΠ°) ΠΈ ΠΎΠ±Π»Π°Π΄Π°ΡΡ Π±Π»ΠΈΠ·ΠΊΠΈΠΌΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΌΠΈ ΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΠΌΠΈ ΡΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄ΡΠΎΠ΄Π΅Π½Π°Π·Π° ΡΠ²Π»ΡΠ΅ΡΡΡ Π΅Π΄ΠΈΠ½ΡΡΠ²Π΅Π½Π½ΡΠΌ ΠΊ Π½Π°ΡΡΠΎΡΡΠ΅ΠΌΡ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΎΡ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΌ Π³ΡΡΠΏΠΏΡ Π³ΡΠ°Π½Π·ΠΈΠΌΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ ΠΏΡΠΎΡΠ΅Π°Π· ΠΊΡΡΠΏΠ½ΠΎΠ³ΠΎ ΡΠΎΠ³Π°ΡΠΎΠ³ΠΎ ΡΠΊΠΎΡΠ°, ΠΊ ΠΊΠΎΡΠΎΡΠΎΠΉ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠ°Ρ Π² Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠΎΡΠ΅Π°Π·Π°. ΠΡΠΎΡΠΎΠΉ ΡΠ°Π·Π΄Π΅Π» Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±Π·ΠΎΡΠ° ΠΏΠΎΡΠ²ΡΡΠ΅Π½ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌ Ρ ΠΈΠΌΠ°Π· ΡΡΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ, Π°Π½Π°Π»ΠΎΠ³ΠΎΠΌ ΠΊΠΎΡΠΎΡΡΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΠΉ Π½Π°ΠΌΠΈ ΡΠ΅ΡΠΌΠ΅Π½Ρ.
Π²ΡΠ²ΠΎΠ΄Ρ.
1. ΠΠ· ΡΠ»ΠΈΠ·ΠΈΡΡΠΎΠΉ Π΄ΡΠΎΠ΄Π΅Π½ΡΠΌΠ° Π±ΡΠΊΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΡΡΠΈΠ½Π½ΠΎΠΉ Ρ ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ Π½Π° ΠΊΠΎΠ»ΠΎΠ½ΠΊΠ΅ Ρ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΠΌ ΡΠΎΠ΅Π²ΡΠΌ ΡΡΠΈΠΏΡΠΈΠ½ΠΎΠ²ΡΠΌ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠΌ Π²ΡΠ΄Π΅Π»Π΅Π½Π° Π½ΠΎΠ²Π°Ρ ΡΠ΅ΡΠΈΠ½ΠΎΠ²Π°Ρ ΠΏΡΠΎΡΠ΅Π°Π·Π°. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ ΠΏΡΠ΅ΠΏΠ°ΡΠ°Ρ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ Π³ΠΎΠΌΠΎΠ³Π΅Π½Π½ΡΠΌ ΠΏΠΎ Π΄Π°Π½Π½ΡΠΌ N-ΠΊΠΎΠ½ΡΠ΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ, ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π°, ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΠΎ-ΡΠ°Π·ΠΎΠ²ΠΎΠΉ Ρ ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΈ Π³Π΅Π»Ρ-ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ.
2. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (MALDI-MS) ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½Π°Ρ ΠΌΠ°ΡΡΠ° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 24,97 ΠΊΠΠ°. Π€Π΅ΡΠΌΠ΅Π½Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠΎΠΌ, ΡΠΎΡΡΠΎΡΡΠΈΠΌ ΠΈΠ· ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ»ΠΈΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΠΏΠΈ.
3. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π° N-ΠΊΠΎΠ½ΡΠ΅Π²Π°Ρ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° (23 Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΡ) — Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ°Ρ ΡΡΠ΅ΠΏΠ΅Π½Ρ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠΉ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΠ²Π°Π΅ΡΡΡ Ρ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΠΌΠΈ ΡΡΠ°ΡΡΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Ρ ΠΈΠΌΠ°Π· ΡΡΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ.
4. ΠΠ° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π΄Π°Π½Π½ΡΡ ΠΏΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Ρ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΉ ΠΏΡΠΎΡΠ΅Π°Π·ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΡ ΠΈ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ² ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ, ΠΏΡΠΎΡΠ²Π»ΡΠ΅ΠΌΠ°Ρ Π΅Ρ (Π² ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡΡ Π 1-Π Π’), Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠ½Π° Π΄Π»Ρ ΡΡΠ΄Π° Ρ ΠΈΠΌΠ°Π·.
5. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅Π΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π° ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ ΠΏΡΠΎΡΠ΅Π°Π·Ρ ΡΡΠ΄Π° ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² ΡΠ΅ΡΠΈΠ½ΠΎΠ²ΡΡ ΠΏΡΠΎΡΠ΅Π°Π·. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ, ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎ Ρ ΠΈΠΌΠ°Π·Π°ΠΌ, ΡΠ΅ΡΠΌΠ΅Π½Ρ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΠ΅ΡΡΡ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ°ΠΌΠΈ ΡΠΈΠΏΠ° ΠΡΠ½ΠΈΡΡΠ° ΠΈ ΠΠ°ΡΠΌΠ°Π½Π°-ΠΠΈΡΠΊ ΠΈΠ· Π±ΠΎΠ±ΠΎΠ²ΡΡ , Π½ΠΎ ΠΏΡΠΎΡΠ²Π»ΡΠ΅Ρ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡ ΠΊ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠΈΠΏΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ° BPTI.
6. Π‘ΠΎΠ²ΠΎΠΊΡΠΏΠ½ΠΎΡΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ Π² ΡΠ°Π±ΠΎΡΠ΅ Π΄Π°Π½Π½ΡΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ, ΡΡΠΎ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π½ΡΠΉ Π½Π°ΠΌΠΈ ΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΠΉ Π² ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΠΎΠΌ ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½Ρ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ Ρ ΠΈΠΌΠ°Π·Π°ΠΌ. ΠΠ»Ρ ΡΡΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΏΡΠ΅Π΄Π»Π°Π³Π°Π΅ΡΡΡ Π½Π°Π·Π²Π°Π½ΠΈΠ΅ Π±ΡΡΡΡ Ρ ΠΈΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½Π°Ρ ΠΏΡΠΎΡΠ΅Π°Π·Π°.
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