ΠΠΈΠΏΠ°Π·Ρ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»: Π ΠΎΠ»Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
![ΠΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΡ: ΠΠΈΠΏΠ°Π·Ρ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ
ΠΌΠΈΡΠ΅Π»Π»: Π ΠΎΠ»Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²](https://gugn.ru/work/3597991/cover.png)
ΠΠ° ΡΠ΅Π³ΠΎΠ΄Π½ΡΡΠ½ΠΈΠΉ Π΄Π΅Π½Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΠΎ-ΠΏΡΠ΅ΠΆΠ½Π΅ΠΌΡ ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π΅ΠΏΡΠΎΡΡΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ, Π° ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΡΠ΄ΠΎΠΌ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΊΠΎΠ², Π²ΠΊΠ»ΡΡΠ°ΡΡΠΈΡ : 1) Π΄ΠΎΡΠΎΠ³ΠΎΠ²ΠΈΠ·Π½Ρ ΠΎΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ²- 2) Π½Π΅ΠΏΡΠΈΠΌΠ΅Π½ΠΈΠΌΠΎΡΡΡ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΡ ΡΡΠ΅Π΄Π°Ρ ΠΈ ΡΠΈΡΡΠ΅ΠΌΠ°Ρ , ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ Π°ΠΌΡΠΈΡΠΈΠ»ΡΠ½ΡΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ, ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΡ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ΅Π°ΠΊΡΠΈΠΉ- 3) ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ … Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- Π‘ΠΠΠ‘ΠΠ Π‘ΠΠΠ ΠΠ©ΠΠΠΠ
- ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- I. ΠΠΠΠΠΠ: Π‘Π’Π ΠΠΠΠΠ Π ΠΠ‘ΠΠΠΠΠΠΠ‘Π’Π ΠΠΠ’ΠΠΠΠΠ
- 1. 1. ΠΠ±ΡΠ°Ρ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π»ΠΈΠΏΠ°Π· ΠΈΠ· ΡΠ°Π·Π½ΡΡ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ²
- 1. 2. ΠΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΠΉ ΠΈ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΡΠΉ ΡΠΎΡΡΠ°Π²
- 1. 3. Π‘ΡΡΡΠΊΡΡΡΠ° Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ° ΠΈ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠ½Π°Ρ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ
- 1. 4. ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° ΠΈ ΠΊΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π»ΠΈΠΏΠ°Π·
- 1. 5. Π€Π°ΠΊΡΠΎΡΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° Π»ΠΈΠΏΠΎΠ»ΠΈΠ·
- 1. 6. Π‘ΡΠ±ΡΡΡΠ°ΡΠ½Π°Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ Π»ΠΈΠΏΠ°Π·
- 1. 7. ΠΠ΅ΡΠΎΠ΄Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π·
- 1. 8. ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π»ΠΈΠΏΠ°Π·
- II. Π‘ΠΠ‘Π’ΠΠΠ« ΠΠΠ ΠΠ©ΠΠΠΠ«Π₯ ΠΠΠ¦ΠΠΠ, ΠΠ₯ ΠΠΠ‘Π’ΠΠΠΠ‘Π’ΠΠ
- 2. 1. Π‘ΠΈΡΡΠ΅ΠΌΡ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π», ΠΎΠ±ΡΠΈΠ΅ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ
- 2. 2. Π€Π΅ΡΠΌΠ΅Π½ΡΡ Π² ΡΠΈΡΡΠ΅ΠΌΠ°Ρ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»: ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈ ΠΎΠ»ΠΈΠ³ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π°
- 2. 3. ΠΠ°ΡΠ°Π»ΠΈΠ· Π»ΠΈΠΏΠ°Π·ΠΎΠΉ Π² ΡΠΈΡΡΠ΅ΠΌΠ°Ρ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- III. ΠΠΠΠΠΠ‘ΠΠΠΠΠΠΠ: Π‘Π’Π ΠΠΠΠΠ Π Π‘ΠΠΠΠ‘Π’ΠΠ
- 3. 1. ΠΠ°Ρ ΠΎΠΆΠ΄Π΅Π½ΠΈΠ΅ Π² ΠΏΡΠΈΡΠΎΠ΄Π΅, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠ΅ ΡΠ΅Π°ΠΊΡΠΈΠΈ ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Π²Π°ΠΆΠ½ΠΎΡΡΡ Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·
- 3. 2. Π‘ΡΡΡΠΊΡΡΡΠ° Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ° ΠΈ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·
- IV. ΠΠΠΠΠ’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠΠΠΠΠΠΠ ΠΠΠ‘Π’Π ΠΠΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠ«Π₯ Π ΠΠΠΠ¦ΠΠ
- ΠΠΠ‘Π’ΠΠΠΠΠΠ ΠΠΠΠΠ§Π
- ΠΠΠ‘ΠΠΠ ΠΠΠΠΠ’ΠΠΠ¬ΠΠΠ― Π§ΠΠ‘Π’
- I. ΠΠΠ’ΠΠ ΠΠΠΠ«
- II. ΠΠΠ’ΠΠΠ«
- 2. 1. Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 2. 2. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 2. 3. Π₯ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²
- 2. 4. Π‘Π΅Π΄ΠΈΠΌΠ΅Π½ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ Π°Π½Π°Π»ΠΈΠ·
- 2. 5. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ Π»ΠΈΠΏΠ°Π·
- 2. 6. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π·
- 2. 7. Π‘ΠΈΠ½ΡΠ΅Π· Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π°ΡΠΈΠΊΠ»ΠΎΠ²ΠΈΡΠ°, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠΉ Π»ΠΈΠΏΠ°Π·ΠΎΠΉ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 2. 8. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ «Π»ΠΈΠΏΠ°Π·Π° / Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π°»
- Π ΠΠΠ£ΠΠ¬Π’ΠΠ’Π« Π ΠΠΠ‘Π£ΠΠΠΠΠΠ―
- I. ΠΠΠ©ΠΠ― Π₯ΠΠ ΠΠΠ’ΠΠ ΠΠ‘Π’ΠΠΠ ΠΠ ΠΠΠΠ ΠΠ’ΠΠ ΠΠΠΠΠ
- 1. 1. ΠΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π»ΠΈΠΏΠ°Π·Π° ΡΠ²ΠΈΠ½ΡΠΈ
- 1. 2. ΠΠΈΠΏΠ°Π·Π° ΠΈΠ· Mucor miehe
- 1. 3. ΠΠΈΠΏΠ°Π·Π° ΠΈΠ· Chromobacterium viscosum
- II. Π ΠΠΠ£ΠΠ―Π¦ΠΠ― ΠΠΠΠΠΠΠ’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠΠΠ‘Π’Π Π€ΠΠ ΠΠΠΠ’ΠΠ
- 2. 1. ΠΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π°
- 2. 1. 1. ΠΠ°Π²ΠΈΡΠΈΠΌΠΎΡΡΡ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· ΠΎΡ ΡΠ ΡΡΠ΅Π΄Ρ, ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ ΡΠ΅Π°ΠΊΡΠΈΠΈ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
- 2. 1. 2. ΠΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΡΠ΅Π°ΠΊΡΠΈΠΉ, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΡ Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ Π² Π²ΠΎΠ΄Π½ΠΎΠΉ ΡΡΠ΅Π΄Π΅ ΠΈ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 2. 1. 3. ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΠΈΠΎΠ½ΠΎΠ² ΠΊΠ°Π»ΡΡΠΈΡ ΠΈ ΠΆΠ΅Π»ΡΠ½ΡΡ ΡΠΎΠ»Π΅ΠΉ Π½Π° Π»ΠΈΠΏΠΎΠ»ΠΈΠ· Π² Π²ΠΎΠ΄Π½ΠΎΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠ΅ ΠΈ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 2. 2. Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΠΎΠ»ΠΈΠ³ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° Π»ΠΈΠΏΠ°Π· Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 2. 3. Π Π΅Π³ΡΠ»ΡΡΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΏΠΏΠ°Π· ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 2. 4. Π₯ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ
- 2. 5. Π‘ΠΎΠ±ΡΡΠ²Π΅Π½Π½Π°Ρ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΡ Π»ΠΈΠΏΠ°Π·
- 2. 6. ΠΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΠ°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π»ΠΈΠΏΠ°Π· Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 2. 1. ΠΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π°
- III. Π‘Π’ΠΠΠΠΠ¬ΠΠΠ‘Π’Π¬ ΠΠΠΠΠ
- IV. Π ΠΠΠ£ΠΠ―Π¦ΠΠ― Π‘ΠΠΠ’ΠΠ’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠΠΠ‘Π’Π ΠΠΠΠΠ
- V. ΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠΠ― Π‘ΠΠ‘Π’ΠΠΠ «ΠΠΠΠΠΠ / ΠΠΠΠΠΠ‘ΠΠΠΠΠΠΠ»
- 5. 1. ΠΠ°ΡΠ°Π»ΠΈΠ· ΡΠΎΠ΅Π²ΠΎΠΉ Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·ΠΎΠΉ-1 Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 5. 2. ΠΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ: ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΊΠΎΡΠΎΡΡΡ Π»ΠΈΠΌΠΈΡΠΈΡΡΡΡΠ΅ΠΉ ΡΡΠ°Π΄ΠΈΠΈ
- 5. 3. ΠΠ°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΊΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΊΠΎΠ½ΡΡΠ°Π½Ρ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΎΡ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π³ΠΈΠ΄ΡΠ°ΡΠ°ΡΠΈΠΈ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ’ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»
- 5. 4. Π’Π΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ°ΡΠ΅Π» Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ «Π»ΠΈΠΏΠ°Π·Π°/ Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π°»
- ΠΠ«ΠΠΠΠ«
ΠΠΈΠΏΠ°Π·Ρ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π»: Π ΠΎΠ»Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΈΠΏΠ°Π·Ρ (Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·Ρ Π²ΡΡΡΠΈΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ²) ΠΈΠ³ΡΠ°ΡΡ ΠΊΠ»ΡΡΠ΅Π²ΡΡ ΡΠΎΠ»Ρ Π² ΠΎΠ±ΠΌΠ΅Π½Π΅ Π»ΠΈΠΏΠΈΠ΄ΠΎΠ² Π²ΡΠ΅Ρ ΠΆΠΈΠ²ΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠ°ΡΡΠ²ΡΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ ΠΎΡΠ»ΠΎΠΆΠ΅Π½ΠΈΡ ΠΈ ΡΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΆΠΈΡΠ°, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΠΎΠ³ΠΎ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π·Π΅ΡΠ²Π° ΠΊΠ»Π΅ΡΠΊΠΈ. ΠΠΈΠΏΠ°Π·Ρ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·ΡΡΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Ρ Π² ΠΏΠΈΡΠ΅Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΡΡΠ°ΠΊΡΠ΅ Π΄ΠΎ Π΄ΠΈ-, ΠΌΠΎΠ½ΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² ΠΈ ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΡΡ ΠΆΠΈΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ, Π½ΠΎ ΡΠ°ΠΊΠΆΠ΅ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΡΠ΅ΡΠ΅ΠΎΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡΡ Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈ Π΄Π΅Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ ΡΠΈΡΠ»Π° ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ², ΠΎΡΠ»ΠΈΡΠ½ΡΡ ΠΎΡ Π³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ². ΠΠΈΠΏΠ°Π·Ρ ΡΡΠ°Π±ΠΈΠ»ΡΠ½Ρ Π² Π²ΠΎΠ΄Π½ΡΡ ΠΈ ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΡ ΡΡΠ΅Π΄Π°Ρ ΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ Ρ Ρ ΠΎΡΠΎΡΠΈΠΌ Π²ΡΡ ΠΎΠ΄ΠΎΠΌ ΠΈΠ· ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ, ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ , Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΠ· ΠΏΡΠΈΡΠΎΠ΄Π½ΡΡ ΠΈ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ°ΡΡ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ². Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π»ΠΈΠΏΠ°Π·Ρ Π½Π°Ρ ΠΎΠ΄ΡΡ ΡΠΈΡΠΎΠΊΠΎΠ΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ ΠΎΠ±Π»Π°ΡΡΡΡ , Π²ΠΊΠ»ΡΡΠ°Ρ Π»Π΅ΡΠ΅Π±Π½ΡΡ ΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΡΡ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ, ΠΏΠΈΡΠ΅Π²ΡΡ, ΠΊΠΎΡΠΌΠ΅ΡΠΈΡΠ΅ΡΠΊΡΡ ΠΈ Π±ΡΠΌΠ°ΠΆΠ½ΡΡ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ, ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²ΠΎ Π΄Π΅ΡΠ΅ΡΠ³Π΅Π½ΡΠΎΠ² ΠΈ ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΈΠ½ΡΠ΅Π·. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅, Π² ΠΏΠΎΡΠ»Π΅Π΄Π½Π΅Π΅ Π²ΡΠ΅ΠΌΡ ΠΎΡΠΎΠ±ΠΎΠ΅ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ΄Π΅Π»ΡΠ΅ΡΡΡ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ Π±ΠΈΠΎΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΡΡΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°.
ΠΡΠΊΠ»ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡΡ Π»ΠΈΠΏΠ°Π·Ρ, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠ΅ΠΉ Π΅Π΅ ΠΎΡ Π΄ΡΡΠ³ΠΈΡ ΡΡΡΠ΅ΡΠ°Π·, ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ²ΠΎΠΉΡΡΠ²ΠΎ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ Π°Π³ΡΠ΅Π³ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ» ΡΡΠ±ΡΡΡΠ°ΡΠ° (ΠΆΠΈΡΠΎΠ²ΡΡ ΠΊΠ°ΠΏΠ΅Π»Ρ), ΡΡΠΎ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠΎΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π΅Π³ΠΎ Π°Π΄ΡΠΎΡΠ±ΡΠΈΠΈ Π½Π° ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠΈΠ΄Π°. Π Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ ΠΈΠΌΠ΅ΡΡΡΡ ΡΠΊΠ°Π·Π°Π½ΠΈΡ Π½Π° ΡΠΎ, ΡΡΠΎ Π°Π΄ΡΠΎΡΠ±ΡΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ Π½Π° Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠΌ ΠΌΠΎΠ½ΠΎΡΠ»ΠΎΠ΅ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π»ΠΈΠΏΠ°Π·ΠΎΠΉ, ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²ΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½Ρ-ΡΡΠ±ΡΡΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°. ΠΡΠ΅Π²ΠΈΠ΄Π½ΠΎ, ΡΡΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ Ρ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΡ Π»ΠΈΠΏΠΈΠ΄Π° Π±ΡΠ΄Π΅Ρ Π·Π°Π²ΠΈΡΠ΅ΡΡ ΠΊΠ°ΠΊ ΠΎΡ ΡΠ²ΠΎΠΉΡΡΠ² ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ (Π·Π°ΡΡΠ΄Π°, ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ, ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ Π½Π°ΡΡΠΆΠ΅Π½ΠΈΡ ΠΈ Ρ. Π΄.), ΡΠ°ΠΊ ΠΈ ΠΎΡ ΡΠ²ΠΎΠΉΡΡΠ² ΡΠ°ΠΌΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΠΎΡ Π½Π°Π»ΠΈΡΠΈΡ Π½Π° Π΅Π³ΠΎ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Π³ΡΡΠΏΠΏ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΈ Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ. ΠΠΎΠΏΡΠΎΡ ΠΎ ΡΠΎΠ»ΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΡ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ, Ρ ΠΎΠ΄Π½ΠΎΠΉ ΡΡΠΎΡΠΎΠ½Ρ, ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ Π΄Π»Ρ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ ΡΠ°Π·Π½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ, Π° Ρ Π΄ΡΡΠ³ΠΎΠΉ ΡΡΠΎΡΠΎΠ½Ρ, ΠΈΠΌΠ΅Π΅Ρ Π²Π°ΠΆΠ½ΠΎΠ΅ ΠΏΡΠΈΠΊΠ»Π°Π΄Π½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅, Π·Π°ΠΊΠ»ΡΡΠ°ΡΡΠ΅Π΅ΡΡ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° in vitro ΠΈ Π² Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ ΠΏΡΠΈ ΡΠ΅ΡΠ΅Π½ΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π±ΠΈΠΎΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ Π·Π°Π΄Π°Ρ. ΠΠ»Ρ ΡΠ΅ΡΠ΅Π½ΠΈΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π²ΠΎΠΏΡΠΎΡΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ Π²Π΅ΡΡΠΌΠ° ΡΠ΄ΠΎΠ±Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ° ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π», ΠΊΠΎΡΠΎΡΠ°Ρ, Π² ΠΎΡΠ»ΠΈΡΠΈΠ΅ ΠΎΡ Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ½ΠΎΡΠ»ΠΎΡ, ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° ΠΊΠ°ΠΊ Π΄Π»Ρ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ ΠΎΠΊΡΡΠΆΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ², ΡΠ°ΠΊ ΠΈ Π΄Π»Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π±ΠΈΠΎΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ². Π ΡΠ»ΡΡΠ°Π΅ ΡΠΈΡΡΠ΅ΠΌΡ ΡΡΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΡ (ΡΠ»ΠΎΠ΅ΠΌ ΠΠΠ) ΠΌΠΎΠΆΠ΅Ρ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°ΡΡΡΡ ΠΊΠ°ΠΊ ΠΏΡΡΠ΅ΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΡ, Π° ΠΈΠΌΠ΅Π½Π½ΠΎ, ΡΠ°Π·ΠΌΠ΅ΡΠ° ΠΈ ΡΠΈΡΠ»Π° ΠΌΠΈΡΠ΅Π»Π», ΡΠ°ΠΊ ΠΈ ΠΏΡΡΠ΅ΠΌ Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ (Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ Π³ΠΈΠ΄ΡΠΎΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π³ΠΈΠ΄ΡΠΎΡΠΎΠ±ΠΈΠ·Π°ΡΠΈΠΈ) ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΡΠ΅Π»ΡΡ ΡΠ°Π±ΠΎΡΡ ΡΠ²ΠΈΠ»ΠΎΡΡ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· ΡΠ°Π½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» ΠΠΠ’ Π² ΠΈΠ·ΠΎΠΎΠΊΡΠ°Π½Π΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΡ (ΡΠΈΡΠ»Π° ΠΈ ΡΠ°Π·ΠΌΠ΅ΡΠ° ΠΌΠΈΡΠ΅Π»Π») ΠΈ ΡΠ²ΠΎΠΉΡΡΠ² ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΎΠ±ΡΠ΅ΠΊΡΠΎΠ² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ Π²ΡΠ±ΡΠ°Π½Ρ ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠ΅ Π½Π° ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅ Π»ΠΈΠΏΠ°Π·Ρ: ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π»ΠΈΠΏΠ°Π·Π° ΡΠ²ΠΈΠ½ΡΠΈ ΠΈ Π»ΠΈΠΏΠ°Π·Ρ ΠΈΠ· Mucor miehei ΠΈ Chromobacterium viscosum.
ΠΠ° ΡΠ΅Π³ΠΎΠ΄Π½ΡΡΠ½ΠΈΠΉ Π΄Π΅Π½Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΠΎ-ΠΏΡΠ΅ΠΆΠ½Π΅ΠΌΡ ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π΅ΠΏΡΠΎΡΡΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ, Π° ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΡΠ΄ΠΎΠΌ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΊΠΎΠ², Π²ΠΊΠ»ΡΡΠ°ΡΡΠΈΡ : 1) Π΄ΠΎΡΠΎΠ³ΠΎΠ²ΠΈΠ·Π½Ρ ΠΎΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ²- 2) Π½Π΅ΠΏΡΠΈΠΌΠ΅Π½ΠΈΠΌΠΎΡΡΡ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΡ ΡΡΠ΅Π΄Π°Ρ ΠΈ ΡΠΈΡΡΠ΅ΠΌΠ°Ρ , ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ Π°ΠΌΡΠΈΡΠΈΠ»ΡΠ½ΡΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ, ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈΠ΅ ΡΡΠ±ΡΡΡΠ°ΡΡ ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΡ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ΅Π°ΠΊΡΠΈΠΉ- 3) ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ², ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΠΊΠΎΡΠΎΡΡΠΌ Π»ΠΈΠΏΠ°Π·Ρ ΠΏΡΠΎΡΠ²Π»ΡΡΡ Π½ΠΈΠ·ΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ, Π΄ΡΡΠ³ΠΎΠΉ Π²Π°ΠΆΠ½ΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ»Π°ΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΠΏΡΠΎΡΡΠΎΠ³ΠΎ Π½Π΅ΠΏΡΠ΅ΡΡΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π·Ρ ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΠΏΡΠΈΡΠΎΠ΄Π½ΠΎΠΌΡ ΡΡΠ±ΡΡΡΠ°ΡΡ (ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Ρ) Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ «Π»ΠΈΠΏΠ°Π·Π° / Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅ΠΈΠ°Π·Π°», Π²ΠΊΠ»ΡΡΠ°ΡΡΠ΅Π³ΠΎ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²ΡΡΠ²ΠΎΠ±ΠΎΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΏΠΎΠ»Π½Π½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΠΎΠΉ ΠΆΠΈΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΈΠ· ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Π° ΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅Π΅ Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π½ΠΎΠ΅ ΠΎΠΊΠΈΡΠ»Π΅Π½ΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠ½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΠΎΠΉ ΠΆΠΈΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ Π΄ΠΎ Π΅Π΅ Π³ΠΈΠ΄ΡΠΎΠΏΠ΅ΡΠΎΠΊΡΠΈΠ΄Π°, ΡΠ΅Π³ΠΈΡΡΡΠΈΡΡΠ΅ΠΌΠΎΠ³ΠΎ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈ.
ΠΡΠ΅Π΄Π»Π°Π³Π°Π΅ΠΌΠ°Ρ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° «Π»ΠΈΠΏΠ°Π·Π° / Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π°» ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ΄ΠΎΠ±Π½ΠΎΠΉ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· ΠΈΠ· ΡΠ°Π·Π½ΡΡ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ², Π΅ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΡΠ±ΡΡΡΠ°ΡΠ° ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Ρ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠ½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΠΉ Π°ΡΠΈΠ»ΡΠ½ΡΠΉ ΡΡΠ°Π³ΠΌΠ΅Π½Ρ Π² ΡΡΡΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π»Π΅Π½Π½ΠΎΠΌ s/i-ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠΈ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Π°. Π£Π½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ Π΄Π°Π½Π½ΠΎΠΉ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ ΡΠ°ΠΊΠΆΠ΅ Π·Π°ΠΊΠ»ΡΡΠ°Π΅ΡΡΡ Π² ΡΠΎΠΌ, ΡΡΠΎ Π·Π°ΠΌΠ΅Π½Π° Π»ΠΈΠΏΠ°Π·Ρ Π½Π° ΡΠΎΡΡΠΎΠ»ΠΈΠΏΠ°Π·Ρ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠ°ΡΡΠΈΡΠΈΡΡ ΠΊΡΡΠ³ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΠΌ ΡΡΠ±ΡΡΡΠ°ΡΠ°ΠΌ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΌ ΠΏΠΎΠ»ΠΈΠ½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΠ΅ ΠΆΠΈΡΠ½ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ.
Π Π½Π°ΡΡΠΎΡΡΠΈΠΉ ΠΌΠΎΠΌΠ΅Π½Ρ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· Π²Π°ΠΆΠ½ΡΡ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ Π·Π°Π΄Π°Ρ Π² ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Π΅ ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ ΡΡΠΎΠΈΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° ΠΏΠ΅ΡΠ΅ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΆΠΈΡΠΎΠ² ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ Π½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΠ΅ ΠΆΠΈΡΠ½ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ. ΠΠΎΠ»ΠΈΠ½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΠ΅ ΠΆΠΈΡΠ½ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ 1,4-ΡΠΈΡ, ΡΠΈΡ-ΠΏΠ΅Π½ΡΠ°Π΄ΠΈΠ΅Π½ΠΈΠ»ΡΠ½ΡΠΉ ΡΡΠ°Π³ΠΌΠ΅Π½Ρ ΠΈ ΡΠ²Π»ΡΡΡΠΈΠ΅ΡΡ ΡΡΠ±ΡΡΡΠ°ΡΠ°ΠΌΠΈ Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ (Π»Π΅ΠΉΠΊΠΎΡΡΠΈΠ΅Π½ΠΎΠ², ΠΏΡΠΎΡΡΠ°Π³Π»Π°Π½Π΄ΠΈΠ½ΠΎΠ², ΡΡΠΎΠΌΠ±ΠΎΠΊΡΠ°Π½ΠΎΠ² ΠΈ Π΄Ρ.), Π²ΡΠΏΠΎΠ»Π½ΡΡΡΠΈΡ Π²Π°ΠΆΠ½ΡΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΈ ΠΈ Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½Π½ΡΡ Π² ΠΈΠΌΠΌΡΠ½Π½ΡΡ ΡΠΈΡΡΠ΅ΠΌΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ ΡΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΡ ΠΈΠΌΠ΅Π½Π½ΠΎ ΡΡΠΈΡ ΠΆΠΈΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΡΠΈΡΠΊ ΠΌΠ½ΠΎΠ³ΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎ-ΡΠΎΡΡΠ΄ΠΈΡΡΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΡΠ°ΠΊΠ° ΠΈ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΡΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ.
ΠΠΎΡΡΠΎΠΌΡ ΠΏΡΠ΅Π΄Π»Π°Π³Π°Π΅ΠΌΠ°Ρ Π½Π°ΠΌΠΈ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° «Π»ΠΈΠΏΠ°Π·Π° / Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π°» ΠΌΠΎΠΆΠ΅Ρ ΠΎΠΊΠ°Π·Π°ΡΡΡΡ ΡΠ΄ΠΎΠ±Π½ΠΎΠΉ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌΠΎΠΉ ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ ΠΎΡΠ΅Π½ΠΈΠ²Π°ΡΡ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ ΠΏΠΈΡΠ΅Π²ΡΡ ΠΌΠ°ΡΠ΅Π» ΠΈ ΠΆΠΈΡΠΎΠ², Π·Π°ΠΊΠ»ΡΡΠ°ΡΡΠ΅Π΅ΡΡ Π² ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠΈ Π² Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π²Π°ΠΆΠ½ΡΡ ΠΏΠΎΠ»ΠΈΠ½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ ΠΆΠΈΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ.
ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«.
I. ΠΠΠΠΠΠ: Π‘Π’Π ΠΠΠΠΠ Π ΠΠ‘ΠΠΠΠΠΠΠ‘Π’Π ΠΠΠ’ΠΠΠΠΠ.
1.1.0Π±ΡΠ°Ρ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π»ΠΈΠΏΠ°Π· ΠΈΠ· ΡΠ°Π·Π½ΡΡ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ².
ΠΠΈΠΏΠ°Π·Π° (Π³ΠΈΠ΄ΡΠΎΠ»Π°Π·Π° ΡΡΠΈΠ°ΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ², Π.Π€. 3.1.1.3) ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΡΠΎΠ±ΠΎΠΉ ΡΠ΅ΡΠΌΠ΅Π½Ρ, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΡΡΠΈΠΉ in vivo Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ· ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² Π΄ΠΎ ΠΌΠΎΠ½ΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² ΠΈ ΠΆΠΈΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ [1]. ΠΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΡΠΎΡΠ΅ΡΡ Π² ΠΏΠΈΡΠ΅Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΡΡΠ°ΠΊΡΠ΅ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΡΡΡ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΡΠΌΠΈ Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ, Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·ΡΡΡΠΈΠΌΠΈ ΠΎΠΊΠΎΠ»ΠΎ 10% ΠΏΠΈΡΠ΅Π²ΡΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² Π΄ΠΎ ΠΆΠΈΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ ΠΈ .ΠΌ-1,2-Π΄ΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ², ΠΈ Π΄Π°Π»Π΅Π΅ ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ, ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΡΡΠΈΠΌΠΈ ΠΏΡΠ΅Π²ΡΠ°ΡΠ΅Π½ΠΈΠ΅ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² ΠΈ sw-1,2-Π΄ΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² Π² ΠΆΠΈΡΠ½ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΈ .ΡΠΈ-2-ΠΌΠΎΠ½ΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ Π»Π΅ΡΠ΅ ΡΡΠ²Π°ΠΈΠ²Π°ΡΡΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠΌ.
ΠΠΈΠΏΠ°Π·Ρ ΡΠ°ΠΊΠΆΠ΅ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΠΏΡΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΡ ΡΡΠ»ΠΎΠ²ΠΈΡΡ ΠΎΠ±ΡΠ°ΡΠ½ΡΡ ΡΠ΅Π°ΠΊΡΠΈΡ Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΆΠΈΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΠΎΠΉ Π³Π»ΠΈΡΠ΅ΡΠΈΠ½Π°, ΠΌΠΎΠ½ΠΎΠΈ Π΄ΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΠ΅ΡΠ΅ΡΡΠ΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² (Π ΠΈΡ.1). ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΠΏΡΠ΅Π΄ΠΏΠΎΡΡΠ΅Π½ΠΈΠ΅ ΠΊ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Π°ΠΌ, Π»ΠΈΠΏΠ°Π·Ρ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·ΡΡΡ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ ΠΎΡΠ»ΠΈΡΠ½ΡΡ ΠΎΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² ΡΡΠ±ΡΡΡΠ°ΡΠΎΠ², ΡΠ°ΠΊΠΈΡ ΠΊΠ°ΠΊ Π°Π»ΠΈΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅, Π°ΡΠΈΠΊΠ»ΠΈΡΠ΅ΡΠΊΠΈΠ΅, Π±ΠΈΡΠΈΠΊΠ»ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ Π°ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠΈΡΡ ΠΈ Π΄Π°ΠΆΠ΅ ΡΡΠΈΡΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΎΡΠ³Π°Π½ΠΎΠΌΠ΅ΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ΅Π½Π΄Π²Π½Ρ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ [2]. ΠΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΡΠ°ΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΡΠΈΡΠ°ΠΌ ΠΈΠ»ΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ°ΠΌ Ρ ΡΠ°Π·Π½ΡΠΌΠΈ Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠ»ΡΠ½ΡΠΌΠΈ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ, Π»ΠΈΠΏΠ°Π·Ρ ΡΠ΅Π°Π³ΠΈΡΡΡΡ Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠ½Π°Π½ΡΠΈΠΎΠΈ ΡΠ΅Π³ΠΈΠΎΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ.
ΠΠΈΠΏΠ°Π·Π° ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΡΠΎΠΊΠΎ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠΌ, ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π½ΡΠΌ Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ [3−5], ΡΠ°ΡΡΠ΅Π½ΠΈΠΈΠΉ [6,7] ΠΈ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² [8−10]. ΠΠΎΠΌΠΌΠ΅ΡΡΠ΅ΡΠΊΠΈ Π΄ΠΎΡΡΡΠΏΠ½ΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ ΠΎΠ±ΡΡΠ½ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡ ΠΈΠ· ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Π° Ρ ΠΏΠΎΡΠ²Π»Π΅Π½ΠΈΠ΅ΠΌ Π³Π΅Π½Π½ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ ΡΠ²Π΅Π»ΠΈΡΠΈΠ»ΠΎΡΡ ΡΠΈΡΠ»ΠΎ Π»ΠΈΠΏΠ°Π·, ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠΌΡΡ ΠΈΠ· ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ [3].
ΠΠ±ΡΡΠ½ΠΎ Π»ΠΈΠΏΠ°Π·Ρ ΡΠ²Π»ΡΡΡΡΡ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΡΠΎΡΡΠ°Π²Π»ΡΡΡΠΈΡ «Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΊΠΎΠΊΡΠ΅ΠΉΠ»Ρ», Π½Π°ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠ³ΠΎ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠΌ Ρ ΡΠ΅Π»ΡΡ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π΅Π³ΠΎ ΡΠΎΡΡΠ°. ΠΡΠΎΡΠ΅Π΄ΡΡΠ° ΠΎΡΠΈΡΡΠΊΠΈ Π»ΠΈΠΏΠ°Π· ΠΎΡ ΡΡΡΠ΅ΡΠ°Π· ΠΈ ΠΏΡΠΎΡΠ΅Π°Π· Π²Π΅ΡΡΠΌΠ° ΡΡΡΠ΄ΠΎΠ΅ΠΌΠΊΠ°, ΡΠ°ΠΊ ΠΊΠ°ΠΊ Π°ΡΡΠΈΠ½Π½ΠΎΡΡΡ Π»ΠΈΠΏΠ°Π· Π²ΡΡΠΎΠΊΠ° Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΊ Π³ΡΠ°Π½ΠΈΡΠ΅ Π²ΠΎΠ΄Π° / ΠΌΠ°ΡΠ»ΠΎ, Π½ΠΎ ΠΈ ΠΊ Π΄ΡΡΠ³ΠΈΠΌ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΠΌ Ρ ΠΌΠ΅Π½ΡΡΠ΅ΠΉ ΠΏΠΎΠ»ΡΡΠ½ΠΎΡΡΡΡ (Π²ΠΎΠ΄Π° / ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ°ΡΡΠ²ΠΎΡΠΈΡΠ΅Π»Ρ, ΡΡΠ΅ΠΊΠ»ΠΎ, ΠΏΠ»Π°ΡΡΠΈΠΊ, Π²ΠΎΠ·Π΄ΡΡΠ½ΡΠ΅ ΠΏΡΠ·ΡΡΠΈ), Π½Π° ΠΊΠΎΡΠΎΡΡΡ Π»ΠΈΠΏΠ°Π·Π° ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ±ΡΠ°ΡΠΈΠΌΠΎ Π°Π΄ΡΠΎΡΠ±ΠΈΡΠΎΠ²Π°ΡΡΡΡ ΠΈ Π΄Π΅Π½Π°ΡΡΡΠΈΡΠΎΠ²Π°ΡΡ [II]. Π³ ΠΈ Π΄ Ρ ΠΎ Π» ΠΈ 3.
OR or Ρ Ρ.
Π Π .
Π Ρ ΠΈ ΠΊ.
Π Π¦
Π Π― r or ΡΡΠΈΠ³Π»Π½ΡΠ΅ΡΠΈΠ΄ r ΠΎΠ½ Π΄ΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ Π³Π»ΠΈΡΠ΅ΡΠΈΠ½ 4r-c00h.
Π ΠΎΠ½ ΠΌΠΎΠ½ΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ r or ΡΡΠΈΠ³Π»ΠΊΡΠ΅ΡΠΊΠ΄.
Π ΠΠ Π³Π»ΠΈΡΠ΅ΡΠΈΠ½.
Π ΠΈΡ. 1. Π Π΅Π°ΠΊΡΠΈΠΈ, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠ΅ Π»ΠΈΠΏΠ°Π·ΠΎΠΉ.
ΠΠ½ΠΎΠ³ΠΈΠ΅ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡ ΡΠΌΠ΅ΡΠΈ Π»ΠΈΠΏΠ°Π·Π½ΡΡ ΠΈΠ·ΠΎΡΠΎΡΠΌ, ΠΈΠΌΠ΅ΡΡΠΈΠ΅ Π½Π΅Π±ΠΎΠ»ΡΡΠΎΠ΅ ΠΎΡΠ»ΠΈΡΠΈΠ΅, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ, Π² ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠΈ ΡΠ³Π»Π΅Π²ΠΎΠ΄ΠΎΠ² ΠΈΠ»ΠΈ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°. ΠΠ΄Π½Π°ΠΊΠΎ, ΠΈΠ·ΠΎΡΠΎΡΠΌΡ Π»ΠΈΠΏΠ°Π· ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΎΡΠ²Π»ΡΡΡ ΡΠ°Π·Π½ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ»ΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ½ΡΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΡΡ ΠΏΠΎ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ (Π’Π°Π±Π». 1).
Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π²ΡΠ΄Π΅Π»Π΅Π½Ρ ΠΈ ΠΎΡ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Ρ Π»ΠΈΠΏΠ°Π·Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΠΎΠ΄Π½Π°ΠΊΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΠΈ ΡΠ°Π½Π΅Π΅ ΠΈΠ·ΡΡΠ΅Π½Π½ΡΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΌ Π»ΠΈΠΏΠ°Π· ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π»ΠΈΠΏΠ°Π·Π° [1].
ΠΠΏΡΠΈΠΌΡΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π° Π»ΠΈΠΏΠ°Π· Π»Π΅ΠΆΠΈΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ 7−9. ΠΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΡΡΠ°Π²Π»ΡΡΡ ΡΠ·ΡΠΊΠΎΠ²ΡΠ΅ ΠΈ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΡΡ , ΡΠΊΠ°Π½Π΅Π²ΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ Π»ΠΈΠΏΠΎΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΠΈ Π»ΠΈΠΏΠ°Π·Ρ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π°ΠΊΡΠΈΠ²Π½Ρ ΠΈ ΡΡΠ°Π±ΠΈΠ»ΡΠ½Ρ ΠΏΡΠΈ ΠΊΠΈΡΠ»ΡΡ ΡΠ [1]. ΡΠ-ΠΎΠΏΡΠΈΠΌΡΠΌ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΡΡ Π»ΠΈΠΏΠ°Π· ΡΠ°Π²Π΅Π½ ΡΠ 5.4 [12] Π² ΠΎΡΠ»ΠΈΡΠΈΠ΅ ΠΎΡ ΡΠ 8−9 Π΄Π»Ρ ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠΈΡ Π»ΠΈΠΏΠ°Π· [13]. ΠΠΈΠΏΠ°Π·Π° ΠΈΠ· ΠΊΠ»Π΅ΡΠ΅Π²ΠΈΠ½Ρ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π°ΠΊΡΠΈΠ²Π½Π° ΠΏΡΠΈ ΡΠ 4.2, Π° Π»ΠΈΠΏΠ°Π·Ρ ΠΈΠ· ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ° Mucorpusillus ΠΏΡΠΎΡΠ²Π»ΡΡΡ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ 5−6 [1].
ΠΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ ΠΌΠΎΠ³ΡΡ Π΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°ΡΡ Π² ΠΎΡΠ΅Π½Ρ ΡΠΈΡΠΎΠΊΠΎΠΌ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡ. ΠΠ°ΠΏΡΠΈΠΌΠ΅Ρ, Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π°ΠΊΡΠΈΠ²Π½Ρ ΠΏΡΠΈ -20Β°Π‘ [1], Π° ΡΠ΅ΡΠΌΠ΅Π½Ρ ΠΈΠ· ΡΠ΅ΠΌΡΠ½ Vernonia anthclm’mthica — ΠΏΡΠΈ 65 Β°C. Π’Π΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΡΠΉ ΠΎΠΏΡΠΈΠΌΡΠΌ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π° Π»ΠΈΠΏΠ°Π· Π»Π΅ΠΆΠΈΡ Π² ΡΠ°ΠΉΠΎΠ½Π΅ 30−37 Β°Π‘.
ΠΠΈΠΏΠ°Π·Π° Π½ΡΠΆΠ΄Π°Π΅ΡΡΡ Π² ΠΈΠΎΠ½Π°Ρ Na+, ΡΠ»ΡΡΡΠ°ΡΡΠΈΡ ΡΠ΅ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ Ρ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΡ Π»ΠΈΠΏΠΈΠ΄Π°, Π° ΡΠ°ΠΊΠΆΠ΅ Π² ΠΈΠΎΠ½Π°Ρ ΠΏΠΎΠ΄Π°Π²Π»ΡΡΡΠΈΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΡΠΌΠΈ ΠΆΠΈΡΠ½ΡΠΌΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ.
Π’Π°Π±Π»ΠΈΡΠ° 1. Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΠΊΠΎΠΌΠΌΠ΅ΡΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π»ΠΈΠΏΠ°Π· [2].
ΠΡΡΠΎΡΠ½ΠΈΠΊ Π»ΠΈΠΏΠ°Π·Ρ Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°.
Candida rugosa ΡΠΎΡΠΌΠ°Π»ΡΠ½ΠΎ Candida cylindracea, ΠΎΡΠΈΡΡΠΊΠ° ΠΈ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π΄Π°Π΅Ρ 5 ΡΠΎΠ΄ΡΡΠ²Π΅Π½Π½ΡΡ ΠΈΠ·ΠΎΡΠΎΡΠΌ Π»ΠΈΠΏΠ°Π·Ρ, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΡ ΡΡ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΏΡΠΎΡΠ²Π»ΡΡΡΠΈΡ ΡΠ°Π·Π½ΡΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΠΊΠΎΡΠΎΡΠΊΠΎΠΈ Π΄Π»ΠΈΠ½Π½ΠΎΡΠ΅ΠΏΠΎΡΠ΅ΡΠ½ΡΠΌ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Π°ΠΌ.
Geotrtchum candidum ΡΠΎΠ΄Π΅ΡΠΆΠΈΡ 2 ΠΈΠ·ΠΎΡΠΎΡΠΌΡ, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΠ΅ΡΡ ΠΏΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΡΠΈΡ-Π9- ΠΠΠΠ.
Rhizopus sp. Π»ΠΈΠΏΠ°Π·Ρ R. arrhizus, R, oryzae, R. delemar ΠΈ R. niveus ΠΈΠΌΠ΅Π΅ΡΡ Π²ΡΡΠΎΠΊΡΡ ΡΡΠ΅ΠΏΠ΅Π½Ρ ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½ΡΡΠΈ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠΎΡΠ²Π»ΡΡΡ ΡΠ°Π·Π½ΡΡ ΡΡΠ±ΡΡΡΠ°ΡΠ½ΡΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΈ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ.
Peniciltium camembertii ΡΠΎΡΠΌΠ°Π»ΡΠ½ΠΎ P. cyclopium, ΡΠΎΠ΄Π΅ΡΠΆΠΈΡ 4 Π»ΠΈΠΏΠ°Π·Π½ΡΡ ΠΈΠ·ΠΎΡΠΎΡΠΌΡ, ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΠΈΠ΅ΡΡ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΡΠΌ ΡΠΎΡΡΠ°Π²ΠΎΠΌ Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π΄ΠΎΠΌΠ΅Π½Π°.
Pseudomonas glumae Π»ΠΈΠΏΠ°Π·Π° ΠΈΠ· ΡΡΠΎΠ³ΠΎ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠ° ΠΈΠΌΠ΅Π΅Ρ 100% ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½ΠΎΡΡΡ Ρ Π»ΠΈΠΏΠ°Π·ΠΎΠΉ ΠΈΠ· Chromobacterium viscosum.
Π²ΡΠ²ΠΎΠ΄Ρ.
1. ΠΠ° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΠΏΠ°Π½ΠΊΡΠ΅Π°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π»ΠΈΠΏΠ°Π·Ρ ΡΠ²ΠΈΠ½ΡΠΈ (PPL), Π³ΡΠΈΠ±Π½ΠΎΠΉ Π»ΠΈΠΏΠ°Π·Ρ ΠΈΠ· ΠΠΈΡΠΎΠ³ miehei (MmL) ΠΈ Π΄Π²ΡΡ ΡΠΎΡΠΌ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π»ΠΈΠΏΠ°Π·Ρ ΠΈΠ· Chromobacterium viscosum (CvL, Π ΠΈ CvL Π) ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΊΠ»ΡΡΠ΅Π²ΡΠΌ ΠΌΠΎΠΌΠ΅Π½ΡΠΎΠΌ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» ΡΠ²Π»ΡΠ΅ΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΡ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΠΌΠΎΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΈ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΠΌΠΎΠ΅ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠ°ΠΌΠΈ ΡΠΈΡΡΠ΅ΠΌΡ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π».
2. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π», ΠΊΠ°ΠΊ ΠΈ Π² Π²ΠΎΠ΄Π½ΠΎΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠ΅, Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΡΠΎΡΠ΅ΡΡ ΠΈΠΌΠ΅Π΅Ρ Π»Π°Π³-ΠΏΠ΅ΡΠΈΠΎΠ΄, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΠΌΡΠΉ ΡΡΠ°Π΄ΠΈΠ΅ΠΉ Π°Π΄ΡΠΎΡΠ±ΡΠΈΠΈ Π»ΠΈΠΏΠ°Π· Π½Π° ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ, ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Ρ ΠΌΠ΅ΠΆΡΠ°Π·Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΡΡ ΠΈ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½Π° Π΄Π»Ρ Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π»ΠΈΠΏΠ°Π· (PPL ΠΈ CvLA) ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π±ΠΎΠ»Π΅Π΅ Π³ΠΈΠ΄ΡΠΎΡΠΎΠ±Π½ΡΠΌΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΠΌΠΈ (MmL ΠΈ CvLB).
3. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π΄Π»Ρ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» Π½Π΅ ΡΡΠ΅Π±ΡΡΡΡΡ ΡΠ°ΠΊΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π°ΡΠΎΡΡ Π»ΠΈΠΏΠΎΠ»ΠΈΠ·Π°, ΠΊΠ°ΠΊ ΠΈΠΎΠ½Ρ ΠΊΠ°Π»ΡΡΠΈΡ ΠΈ ΡΠΎΠ»ΠΈ ΠΆΠ΅Π»ΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ, Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΡΠ΅ Π΄Π»Ρ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π² Π²ΠΎΠ΄Π½ΠΎΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠ΅. Π ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π²ΠΎΠ΄Π½ΡΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΡΡΠ΅ΠΊΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΡΠ±ΡΡΡΠ°ΡΠΎΠΌ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠ° Π½Π΅ΠΎΠ±ΡΠ°ΡΠΈΠΌΠΎΠ³ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π»ΠΈΠΏΠ°Π·Ρ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠΌ ΡΠ΅Π°ΠΊΡΠΈΠΈ (ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΠΎΠΉ ΠΆΠΈΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΠΎΠΉ) ΠΏΡΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ.
4. Π ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» Π°Π½Π°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΡΡΡ Π² Π²ΠΈΠ΄Π΅ Π²ΡΡΠΎΠΊΠΎΠ°ΠΊΡΠΈΠ²Π½ΡΡ ΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΡΡ ΠΈ ΡΠ΅ΡΠ°ΠΌΠ΅ΡΠ½ΡΡ ΡΠΎΡΠΌ, ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΡΡΡ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π³ΠΈΠ΄ΡΠ°ΡΠ°ΡΠΈΠΈ ΠΌΠΈΡΠ΅Π»Π» (ΠΈΡ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠΌ).
5. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Π²ΡΠ΅ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡ Ρ ΠΌΠΈΡΠ΅Π»Π»ΡΡΠ½ΠΎΠΉ ΠΌΠ°ΡΡΠΈΡΠ΅ΠΉ, ΠΎΠ΄Π½Π°ΠΊΠΎ, Ρ Π°ΡΠ°ΠΊΡΠ΅Ρ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ (ΡΠΈΡΠ»Π° ΠΌΠΈΡΠ΅Π»Π») Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΠ²ΠΎΠΉΡΡΠ² ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² ΠΈ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ΅ΡΡΡ Π±Π΅ΡΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΡΠΌ ΡΠΈΠΏΠΎΠΌ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π°ΠΌΠΈ ΠΠΠ’ Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π»ΠΈΠΏΠ°Π· (PPL ΠΈ ΡΠ΅ΡΡΠ°ΠΌΠ΅ΡΠ° CvL Π) ΠΈ ΡΠΌΠ΅ΡΠ°Π½Π½ΡΠΌ ΡΠΈΠΏΠΎΠΌ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΠΈΠΌΠΈ Π³ΠΈΠ΄ΡΠΎΡΠΎΠ±Π½ΡΡ Π»ΠΈΠΏΠ°Π· (MmL ΠΈ CvL Π).
6. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Ρ Π°ΡΠ°ΠΊΡΠ΅Ρ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π·Ρ ΠΎΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» ΠΌΠΎΠΆΠ½ΠΎ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠ΅ΠΉ (Π³ΠΈΠ΄ΡΠΎΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ ΠΈ Π³ΠΈΠ΄ΡΠΎΡΠΎΠ±ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ) ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°. ΠΠΈΠ΄ΡΠΎΡΠΎΠ±ΠΈΠ·Π°ΡΠΈΡ MmL ΠΎΡΡΠ°ΡΠΊΠ°ΠΌΠΈ ΠΏΠ°Π»ΡΠΌΠΈΡΠΈΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠ° Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π°ΠΌΠΈ ΠΠΠ’ (ΡΠΌΠ΅ΡΠ°Π½Π½ΡΠΉ ΡΠΈΠΏ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ Ci6-MmL), ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Π³ΠΈΠ΄ΡΠΎΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΡ MmL ΡΠ΅Π»Π»ΠΎΠ±ΠΈΠΎΠ·ΠΎΠΉ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π±Π΅ΡΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π°ΠΌΠΈ ΠΠΠ’ ΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠ° ΠΈ ΡΠ΅ΡΡΠ°ΠΌΠ΅ΡΠ° ΡΡΠΎΠΉ ΡΠΎΡΠΌΡ Π»ΠΈΠΏΠ°Π·Ρ (CB-MmL), ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ.
7. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ MmL Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠ° ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΎΠ»ΠΈΠ³ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π», Π½ΠΎ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ Π½Π΅Π±ΠΎΠ»ΡΡΠΎΠΌΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π·Ρ.
8. Π‘ΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ Π»ΠΈΠΏΠ°Π· Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π» Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΠ²ΠΎΠΉΡΡΠ² ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΈ Π΅Π³ΠΎ ΠΎΠ»ΠΈΠ³ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π°, ΠΏΡΠΈ ΡΡΠΎΠΌ Π»ΠΈΠΏΠ°Π·Ρ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΡΠ΅ Π³ΡΡΠΏΠΏΡ (PPL ΠΈ CB-MmL), Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΠ°Π±ΠΈΠ»ΡΠ½Ρ Π² Π²ΠΈΠ΄Π΅ ΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠΎΠ², Π° Π½Π΅Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π»ΠΈΠΏΠ°Π·Ρ (MmL ΠΈ CvLB) ΠΈ Π»ΠΈΠΏΠ°Π·Ρ, Π½Π° ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ ΠΊΠΎΡΠΎΡΡΡ ΠΈΠΌΠ΅ΡΡΡΡ ΠΎΡΡΠ°ΡΠΊΠΈ ΠΆΠΈΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ (CvLA ΠΈ C]6-MmL), Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΠ°Π±ΠΈΠ»ΡΠ½Ρ Π² Π²ΠΈΠ΄Π΅ ΡΠ΅ΡΡΠ°ΠΌΠ΅ΡΠΎΠ². ΠΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½Π°Ρ Π³ΠΈΠ΄ΡΠΎΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΡ MmL ΡΠ΅Π»Π»ΠΎΠ±ΠΈΠΎΠ·ΠΎΠΉ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΡ ΡΠ΄ΡΠ΅Π½ΠΈΡ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π·Ρ, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ Π΅Π΅ Π³ΠΈΠ΄ΡΠΎΡΠΎΠ±ΠΈΠ·Π°ΡΠΈΡ ΠΎΡΡΠ°ΡΠΊΠ°ΠΌΠΈ ΠΏΠ°Π»ΡΠΌΠΈΡΠΈΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ — ΠΊ ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°.
9. ΠΠ° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΡΡΠ΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π°ΡΠΈΠΊΠ»ΠΎΠ²ΠΈΡΠ° Π»ΠΈΠ½ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΠΎΠΉ, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΠΎΠΉ Π»ΠΈΠΏΠ°Π·Π°ΠΌΠΈ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π», ΠΏΠΎΠΊΠ°Π·Π°Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π²ΡΡ ΠΎΠ΄Π° ΠΏΡΠΎΠ΄ΡΠΊΡΠ° ΡΠ΅Π°ΠΊΡΠΈΠΈ (Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°ΠΏΠ½ΠΎΠ³ΠΎ Π°ΡΠΈΠΊΠ»ΠΎΠ²ΠΈΡΠ°) ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ’ Π΄Π»Ρ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π³ΠΈΠ±ΡΠΎΡΠΎΠ±Π½ΠΎΠΉ Π»ΠΈΠΏΠ°Π·ΠΎΠΉ (MmL) ΠΈ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ’ Π΄Π»Ρ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π° Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π»ΠΈΠΏΠ°Π·ΠΎΠΉ (PPL).
10. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΠΏΡΠΈΡΠΎΠ΄Π½ΡΠΌ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Π°ΠΌ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ «Π»ΠΈΠΏΠ°Π·Π° / Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π°». Π‘ ΠΏΠΎΠΌΠΎΡΡΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΈΠ·ΡΡΠ΅Π½Π° ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ ΠΊΠ°ΡΠ°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠΏΠ°Π· ΡΠ°Π·Π½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π³ΠΈΠ΄ΡΠ°ΡΠ°ΡΠΈΠΈ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΡΡ ΠΌΠΈΡΠ΅Π»Π».
11. ΠΠΎΠΊΠ°Π·Π°Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π±ΠΈΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ «Π»ΠΈΠΏΠ°Π·Π° / Π»ΠΈΠΏΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Π°» Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΏΡΠΎΡΡΠΎΠΉ ΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠ½ΠΎΠΉ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌΡ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π²Π°ΠΆΠ½ΡΡ ΠΏΠΎΠ»ΠΈΠ½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ ΠΆΠΈΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡ Π² ΡΠΎΡΡΠ°Π²Π΅ ΠΏΠΈΡΠ΅Π²ΡΡ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ².
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