Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
ΠΠ°ΠΎΠ±ΠΎΡΠΎΡ, ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ U3 ΠΎΠ±Π»Π°ΡΡΡ LTR ΠΈΠ· Π»ΠΎΠΊΡΡΠ° ΠΠ‘52 374 Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π² ΡΠ΅ΠΌΠΈΠ½ΠΎΠΌΠ΅ ΠΈ ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ΅, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ U5 ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ ΡΡΠΎΠ³ΠΎ LTR Π½Π΅ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½Π΅ΠΉ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ LTR Π½Π° ΠΏΠ°Π½Π΅Π»ΠΈ ΠΊΠΠΠ ΠΈΠ· ΡΠ΅ΡΡΠΈ ΡΠΊΠ°Π½Π΅ΠΉ ΠΈ ΡΡΠ΅Ρ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΠΉ ΡΡΠΌΠΌΠΈΡΠΎΠ²Π°Π½Ρ Π² ΡΠ°Π±Π»ΠΈΡΠ΅ 2.1. ΠΠ· ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ … Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΠΠΠΠ 1. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- ΠΠ»Π°ΡΡΡ ΡΠ΅ΡΡΠΎΡΡΠ°Π½ΡΠΏΠΎΠ·ΠΎΠ½ΠΎΠ²
- 1. LINE ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ
- 1. 1. Π‘ΡΡΡΠΊΡΡΡΠ° ΠΈ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ L1 ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ²
- 1. 2. Π Π°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ L1 ΠΏΠΎ Π³Π΅Π½ΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 1. 3. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ L
- 1. LINE ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ
- 2. ΠΠ½Π΄ΠΎΠ³Π΅Π½Π½ΡΠ΅ ΡΠ΅ΡΡΠΎΠ²ΠΈΡΡΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 2. 1. Π‘ΡΡΠΎΠ΅Π½ΠΈΠ΅ HERV
- 2. 2. ΠΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ
- 2. 3. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΡ ΠΈ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ HERV ΠΈ LTR Π² Π³Π΅Π½ΠΎΠΌΠ΅
- 2. 4. Π Π°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ HERV ΠΈ LTR ΠΏΠΎ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΠ°ΠΌ
- 2. 5. ΠΠΊΡΠΈΠ²Π½ΠΎΡΡΡ HERV ΠΈ ΠΎΠ΄ΠΈΠ½ΠΎΡΠ½ΡΡ LTR
- 2. 6. Π€Π°ΠΊΡΠΎΡΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ HERV
- 2. 7. ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ΅ ΡΡΠ°ΡΡΠΈΠ΅ ERV Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΏΠ°ΡΠΎΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
- 2. 1. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° HERV-K
- 2. 2. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR HERV-K Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 2. 3. ΠΠΎΠΈΡΠΊ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΡ Π΄Π»Ρ ΠΎΠΏΡΡ ΠΎΠ»Π΅ΠΉ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΈ LTR HERV-K ΠΈ LINE
- 3. 1. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ
- 3. 1. 1. Π€Π΅ΡΠΌΠ΅Π½ΡΡ ΠΈ ΡΠ΅Π°ΠΊΡΠΈΠ²Ρ
- 3. 1. 2. ΠΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½ΠΈΠ΅
- 3. 1. 3. Π Π°ΡΡ ΠΎΠ΄Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ
- 3. 1. 4. ΠΡΡΠ΅ΡΠ½ΡΠ΅ ΠΈ Π΄ΡΡΠ³ΠΈΠ΅ ΡΠ°ΡΡΠ²ΠΎΡΡ
- 3. 1. 5. ΠΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ΅Π΄Ρ
- 3. 1. 6. Π’ΠΊΠ°Π½ΠΈ
- 3. 1. 7. ΠΡΠ°ΠΉΠΌΠ΅ΡΡ, ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ²
- 3. 2. ΠΠ΅ΡΠΎΠ΄Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
- 3. 2. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π ΠΠ ΠΈ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ
- 3. 2. 2. ΠΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΠΠΠ°Π·ΠΎΠΉ
- 3. 2. 3. Π‘ΠΈΠ½ΡΠ΅Π· ΠΏΠ΅ΡΠ²ΠΎΠΉ ΡΠ΅ΠΏΠΈ ΠΊΠΠΠ
- 3. 2. 4. PCR
- 3. 2. 5. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΡΡΡΡΠΊΡΡΡΡ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ² ΠΠΠ ΠΈ PCR ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ²
- 3. 2. 6. ΠΠΎΠ΄Π±ΠΎΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ
- 3. 2. 7. ΠΠΎΠΈΡΠΊ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΠΉ, ΠΊΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π² Π³Π΅Π½ΠΎΠΌΠ΅ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 3. 2. 8. ΠΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠΉ Π΄ΠΈΡΠΏΠ»Π΅ΠΉ SDDIR (Selective Differential Display of RNAs containing Interspersed Repeats)
- 3. 2. 9. ΠΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ TGDA (Targeted Genomic Difference Analysis)
Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΊΠΎΠ»ΠΎ 40% Π³Π΅Π½ΠΎΠΌΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠΎΡΡΠ°Π²Π»ΡΡΡ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΠ΅ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΠΌΠΈ (Π΄Π°Π»Π΅Π΅ Π Π), ΡΠ°ΠΊΠΈΠΌΠΈ ΠΊΠ°ΠΊ ΠΊΠΎΡΠΎΡΠΊΠΈΠ΅ Π΄ΠΈΡΠΏΠ΅ΡΠ³ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ²ΡΠΎΡΡ (Short Interspersed Nuclear Elements, SINEs), Π΄Π»ΠΈΠ½Π½ΡΠ΅ Π΄ΠΈΡΠΏΠ΅ΡΠ³ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ²ΡΠΎΡΡ (Long Interspersed Nuclear Elements, LINEs) ΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠΌΠΈ ΡΠ½Π΄ΠΎΠ³Π΅Π½Π½ΡΡ ΡΠ΅ΡΡΠΎΠ²ΠΈΡΡΡΠΎΠ² (Human Endogenous Retroviruses, HERV), ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΌΠΈ Π΄Π»ΠΈΠ½Π½ΡΠ΅ ΠΊΠΎΠ½ΡΠ΅Π²ΡΠ΅ ΠΏΠΎΠ²ΡΠΎΡΡ (Long Terminal Repeats, LTRs), Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΠ΅ Π±Π΅Π»ΠΊΠΈ, ΡΠΎΡΡΠ°Π²Π»ΡΡΡ ΠΌΠ΅Π½Π΅Π΅ 5% Π³Π΅Π½ΠΎΠΌΠ° [1, 2].
Π§Π°ΡΡΡ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π³Π΅Π½ΠΎΠΌΠ° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎ Π°ΠΊΡΠΈΠ²Π½Π°. ΠΠΎΠ»ΡΡΠΎΠ΅ ΡΠΈΡΠ»ΠΎ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΌΠΎΠ³ΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²ΡΠ²Π°ΡΡ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΠ°ΡΡΠ²ΡΡΡ Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΊΠΈ. Π Π΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΠΌΠΎΠ³ΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²ΡΠ²Π°ΡΡ Π½Π΅ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΠ΅ Π ΠΠ, ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ ΡΠΏΠΎΡΠΎΠ±Π½ΡΠ΅ ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π³Π΅Π½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ ΠΏΡΠΈ ΠΏΠΎΡΡΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΌ ΡΠΏΠ»Π°ΠΉΡΠΈΠ½Π³Π΅ Π³Π΅Π½ΠΎΠ² ΠΈΠ»ΠΈ ΡΡΠ°ΡΡΠ²ΡΡ Π² ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠ°Π½ΡΠΏΠΎΠ·ΠΎΠ½ΠΎΠ² ΠΈ Π΄ΡΡΠ³ΠΈΡ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² [3−5]. Π ΠΠ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ Alu ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ, ΡΠΎΡΡΠ°Π²Π»ΡΡΡ 5% ΠΎΡ ΠΈΠ·Π²Π΅ΡΡΠ½ΡΡ ΠΌΠ ΠΠ [6]. Π 5' ΠΈ 3' UTR ΠΌΠ½ΠΎΠ³ΠΈΡ Π³Π΅Π½Π½ΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² ΠΏΡΠΈΡΡΡΡΡΠ²ΡΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ². ΠΡΠΎΠ±Π΅Π½Π½ΠΎ Π±ΠΎΠ³Π°ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΠΌΠΈ Π Π ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΡ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΠΎ ΠΌΠΎΠ»ΠΎΠ΄ΡΡ ΠΊΠ»Π°ΡΡΠΎΠ² Π³Π΅Π½ΠΎΠ², ΡΠ°ΠΊΠΈΡ ΠΊΠ°ΠΊ Π³Π΅Π½Ρ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° ΠΈΠ»ΠΈ ΠΎΡΠ²Π΅ΡΠ° Π½Π° Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π²Π½Π΅ΡΠ½ΠΈΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ² [7].
ΠΠ΅ΡΠ΅ΠΌΠ΅ΡΠ°ΡΡΡ, Π Π ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π²Π»ΠΈΡΡΡ Π½Π° ΡΡΡΡΠΊΡΡΡΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Ρ ΠΎΠ·ΡΠΈΠ½Π° ΠΈ ΠΈΠΌΠ΅ΡΡ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ. Π‘ΡΠΈΡΠ°ΡΡ, ΡΡΠΎ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΡ ΡΠΏΠΎΠ½ΡΠ°Π½Π½ΡΡ ΠΌΡΡΠ°ΡΠΈΠΉ ΠΌΠΎΠΆΠ΅Ρ ΡΠ²Π»ΡΡΡΡΡ ΡΡΠ°Π½ΡΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π³Π΅Π½ΠΎΠΌΠ° [8]. Π―Π²Π»ΡΡΡΡ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π°, Π Π ΠΌΠΎΠ³ΡΡ, ΠΊΠ°ΠΊ ΠΈΠ·ΠΌΠ΅Π½ΡΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π³Π΅Π½Π°, ΡΠ°ΠΊ ΠΈ ΠΈΠ·ΠΌΠ΅Π½ΡΡΡ Π΅Π³ΠΎ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ, Π²Π»ΠΈΡΡΡ Π½Π° ΡΠΊΠ°Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½Π°. ΠΠ»Ρ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠ½ΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ°ΠΌΠΈ ΠΈ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ°ΠΌΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Π±Π»ΠΈΠ·Π»Π΅ΠΆΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π³Π΅Π½ΠΎΠ², ΡΡΠ°ΡΡΠ²ΠΎΠ²Π°ΡΡ Π² Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΠΎΠΌ ΡΠΏΠ»Π°ΠΉΡΠΈΠ½Π³Π΅, ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°ΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π Π Π² ΡΠ΅ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Ρ ΡΠΎΠΌΠ°ΡΠΈΠ½Π° ΠΈ Π΄ΡΡΠ³ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ [5, 9, 10].
ΠΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π±Π»ΠΎΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ PCR Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π Π ΠΌΠΎΠΆΠ΅Ρ ΠΈΠ·ΠΌΠ΅Π½ΡΡΡΡΡ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ Π²Π½Π΅ΡΠ½ΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΈ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΊΠ»Π΅ΡΠΊΠΈ [10]. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° Alu ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΠΏΡΠΈ ΡΡΡΠ΅ΡΡΠΎΠ²ΡΡ ΡΡΠ»ΠΎΠ²ΠΈΡΡ [11]. ΠΡΡΠ³ΠΈΠΌ Ρ ΠΎΡΠΎΡΠΎ ΠΈΠ·Π²Π΅ΡΡΠ½ΡΠΌ ΠΏΡΠΈΠΌΠ΅ΡΠΎΠΌ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΊΠ°ΡΡΠΈΠ½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠ½Π΄ΠΎΠ³Π΅Π½Π½ΡΡ ΡΠ΅ΡΡΠΎΠ²ΠΈΡΡΡΠΎΠ² ΠΈ LINE1 ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ [12, 13]. ΠΡΠ΅ ΡΡΠΈ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ, ΠΏΠΎ ΠΊΡΠ°ΠΉΠ½Π΅ΠΉ ΠΌΠ΅ΡΠ΅, Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ ΡΠΎΡ ΡΠ°Π½ΠΈΠ»ΠΈ ΡΠ²ΠΎΠΉ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π», Π½ΠΎ Π½Π΅ Π΄Π°ΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΎΡΠ²Π΅ΡΠΈΡΡ Π½Π° Π²ΠΎΠΏΡΠΎΡ, ΠΊΠ°ΠΊΠΎΠ²Ρ ΡΡΠ½ΠΊΡΠΈΠΈ Π Π Π² Π³Π΅Π½ΠΎΠΌΠ΅ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΡΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ½ΡΠΌ, ΠΊΠ°ΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ ΡΡΠ°ΡΡΠ²ΡΡΡ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΡΠΊΠΎΠ»ΡΠΊΠΎ ΠΈ ΠΊΠ°ΠΊΠΈΠ΅ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°ΡΡΡΡ ΡΡΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ, ΠΊΠ°ΠΊ ΠΈ Π·Π° ΡΡΠ΅Ρ ΡΠ΅Π³ΠΎ ΠΏΡΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΡ Π²Π½Π΅ΡΠ½ΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΈΠ·ΠΌΠ΅Π½ΡΠ΅ΡΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ Π Π.
Π‘ΡΡΠ΅ΡΡΠ²ΡΠ΅Ρ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΡΠΈΡΠ»ΠΎ ΡΠ°Π±ΠΎΡ, ΠΈΡΡΠ»Π΅Π΄ΡΡΡΠΈΡ ΡΠΎΠ»Ρ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΊΠΈ, Π½ΠΎ Π²ΡΠ΅ ΠΎΠ½ΠΈ ΡΡΠΎΠΊΡΡΠΈΡΠΎΠ²Π°Π½Ρ Π½Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² [5, 9, 10]. ΠΠ° ΡΠ΅Π³ΠΎΠ΄Π½ΡΡΠ½ΠΈΠΉ Π΄Π΅Π½Ρ Π½Π΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΠΎΠ»Π½ΠΎΠ³Π΅Π½ΠΎΠΌΠ½ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎ Π°ΠΊΡΠΈΠ²Π½ΡΡ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ , ΡΠ°ΠΊ ΠΊΠ°ΠΊ Π½Π΅ ΡΡΡΠ΅ΡΡΠ²ΡΠ΅Ρ ΠΌΠ΅ΡΠΎΠ΄Π° Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π²ΡΠ΅Π³ΠΎ ΠΏΡΠ»Π° Π ΠΠ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠΉ ΠΊΠ»Π°ΡΡ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠ΅Π³ΠΎ ΡΡΠ°Π²Π½ΠΈΠ²Π°ΡΡ ΡΠΏΠ΅ΠΊΡΡΡ Π ΠΠ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΠΈΠΏΠ°Ρ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΡΠΊΠ°Π½Π΅ΠΉ.
Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΌΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½Ρ Π΄Π»Ρ Π²ΡΡΡΠ½Π΅Π½ΠΈΡ Π²ΠΊΠ»Π°Π΄Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π Π Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ° Ρ ΠΎΠ·ΡΠΈΠ½Π°, ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΈΡ Π΄Π»Ρ ΠΏΠΎΠ»Π½ΠΎΠ³Π΅Π½ΠΎΠΌΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ HERV ΠΈ LINE ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΈ Π΅Π΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΠΏΡΠΈ ΠΏΠ΅ΡΠ΅Ρ ΠΎΠ΄Π΅ ΠΎΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΡΠΊΠ°Π½Π΅ΠΉ ΠΊ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΠΌ.
ΠΠ«ΠΠΠΠ«:
1. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° HERV-K ΠΌΠ΅Π½ΡΠ΅ΡΡΡ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΠΈΠΏΠ° ΡΠΊΠ°Π½ΠΈ, ΠΏΡΠΈ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ ΠΌΠΈΡΠΎΠ³Π΅Π½ΠΎΠ² ΠΈ ΡΡΡΠ΅ΡΡΠΎΠ²ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ. ΠΠ°ΠΆΠ΄ΡΠΉ LTR ΠΈΠΌΠ΅Π΅Ρ ΡΠ²ΠΎΠΉ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΡΠΉ ΠΏΡΠΎΡΠΈΠ»Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ.
2. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΏΠ΅ΠΊΡΡΠΎΠ² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΠ΅ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ Π³Π΅Π½ΠΎΠΌΠ°, Π² ΡΠ°Π·Π½ΡΡ ΡΠΊΠ°Π½ΡΡ (Π‘Π΅Π»Π΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠΉ ΠΠΈΡΠΏΠ»Π΅ΠΉ Π ΠΠ, SDDIR). ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ SDDIR ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡΡ.
3. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ SDDIR ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈΠΌΠ΅ΡΠ½ΠΎ 10% LTR HERV-K Π²Ρ ΠΎΠ΄ΡΡ Π² ΡΠΎΡΡΠ°Π² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ², ΠΏΡΠΈΡΠ΅ΠΌ ΠΏΡΠΈ ΠΏΠ΅ΡΠ΅Ρ ΠΎΠ΄Π΅ ΠΎΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΊ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΠΈΡ ΠΊΠ°ΠΊ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅, ΡΠ°ΠΊ ΠΈ ΡΡΠΈΠ»Π΅Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ LTR-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ Π ΠΠ.
4. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΠΏΠ΅ΡΠ΅Ρ ΠΎΠ΄Π΅ ΠΎΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΊ Π³Π΅ΡΠΌΠΈΠ½ΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΎΠΏΡΡ ΠΎΠ»ΠΈ Π½Π΅ ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΠΈΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΡΠ°ΡΡΠΎΡΡ ΡΠ΅ΡΡΠΎΠΏΠΎΠ·ΠΈΡΠΈΠΉ LTR HERV-K ΠΈ LINE-1 ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ².
ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅
.
Π‘ ΠΌΠΎΠΌΠ΅Π½ΡΠ° ΠΎΡΠΊΡΡΡΠΈΡ ΠΌΠΎΠ±ΠΈΠ»ΡΠ½ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΏΡΠΎΡΠ»ΠΎ ΠΌΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΡΡΠ½ΠΊΡΠΈΠΈ ΡΡΠΎΠΉ «ΠΈΠ·Π±ΡΡΠΎΡΠ½ΠΎΠΉ» ΠΠΠ Π΄ΠΎ ΡΠΈΡ ΠΏΠΎΡ Π½Π΅ Π²ΠΏΠΎΠ»Π½Π΅ ΡΡΠ½Ρ, Ρ ΠΎΡΡ Π΅Π΅ ΡΡΡΡΠΊΡΡΡΠ° ΠΈΠ·ΡΡΠ΅Π½Π° Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΠΏΠΎΠ΄ΡΠΎΠ±Π½ΠΎ. ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΠΈΡΠΎΠΊΡΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π² Π³Π΅Π½ΠΎΠΌΠ΅ ΡΡΠΊΠ°ΡΠΈΠΎΡ ΠΈ ΠΈΡ ΠΎΡΠ΅Π²ΠΈΠ΄Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΠΎ Π²ΡΠ΅ΠΌΡ ΠΆΠΈΠ·Π½Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΈ Π΄ΡΡΠ³ΠΈΡ Π½Π΅ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π³Π΅Π½ΠΎΠΌΠ° ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅ΠΏΠΎΠ½ΡΡΠ½ΡΠΌ. ΠΡΠ·ΡΠ²Π°Π΅Ρ ΡΠΎΠΌΠ½Π΅Π½ΠΈΠ΅ ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎΡΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎ ΠΎΠ±ΡΡΠΆΠ΄Π°Π΅ΠΌΠΎΠΉ Π² Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ Π³ΠΈΠΏΠΎΡΠ΅Π·Ρ ΠΎΠ± «ΡΠ³ΠΎΠΈΡΡΠΈΡΠ½ΠΎΡΡΠΈ» ΠΈΠ·Π±ΡΡΠΎΡΠ½ΠΎΠΉ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ, Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠ΅ Ρ ΠΊΠΎΡΠΎΡΠΎΠΉ Π²ΡΡ ΠΈΠ·Π±ΡΡΠΎΡΠ½Π°Ρ ΠΠΠ ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π΅Π½ΠΎΠΌΠ½ΡΠΌ ΠΏΠ°ΡΠ°Π·ΠΈΡΠΎΠΌ ΠΈ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½ΡΠ΅ΡΡΡ Π² Π³Π΅Π½ΠΎΠΌΠ΅ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΡΠ°Π½ΡΠΏΠΎΠ·ΠΈΡΠΈΠΉ ΡΠΎΡΠ½ΡΡ ΠΊΠΎΠΏΠΈΠΉ Π½Π΅ΠΌΠ½ΠΎΠ³ΠΎΡΠΈΡΠ»Π΅Π½Π½ΡΡ ΠΈΡΡ ΠΎΠ΄Π½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ.
ΠΠ΅ΡΠ΅ΠΌΠ΅ΡΠ°ΡΡΡ ΠΏΠΎ Π³Π΅Π½ΠΎΠΌΡ, Π Π ΠΌΠΎΠ³ΡΡ Π²Π»ΠΈΡΡΡ Π½Π° ΡΡΡΡΠΊΡΡΡΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Ρ ΠΎΠ·ΡΠΈΠ½Π° ΠΈ ΠΈΠΌΠ΅ΡΡ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ Π΄Π»Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ.
Π‘ΡΡΠ΅ΡΡΠ²ΡΠ΅Ρ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²ΠΎ ΠΏΡΠΈΠΌΠ΅ΡΠΎΠ² Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π²ΡΡΡΠ°ΠΈΠ²Π°Π½ΠΈΡ Π Π ΡΡΠ΄ΠΎΠΌ ΠΈΠ»ΠΈ Π½Π΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠΉ ΡΡΠ°ΡΡΠΎΠΊ Π³Π΅Π½Π°. ΠΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½Π½ΡΠ΅ Π² ΠΎΠ±Π·ΠΎΡΠ΅ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ Π΄Π°Π½Π½ΡΠ΅ ΠΎΠΏΠΈΡΡΠ²Π°ΡΡ Π»ΠΈΡΡ Π΅Π΄ΠΈΠ½ΠΈΡΠ½ΡΠ΅ ΡΠ»ΡΡΠ°ΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π Π, ΠΈ ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ½ΡΠΌ, ΡΠ²Π»ΡΠ΅ΡΡΡ Π»ΠΈ ΡΠ°ΠΊΠ°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ²ΠΎΠΉΡΡΠ²ΠΎΠΌ Π²ΡΠ΅Π³ΠΎ ΠΊΠ»Π°ΡΡΠ° Π Π ΠΈΠ»ΠΈ ΡΠΎΠ»ΡΠΊΠΎ ΠΊΠΎΠ½ΠΊΡΠ΅ΡΠ½ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ².
ΠΡΠΈΠ²Π΅Π΄Π΅Π½Π½ΡΠ΅ Π² ΠΎΠ±Π·ΠΎΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ, ΠΏΠΎ ΠΊΡΠ°ΠΉΠ½Π΅ΠΉ ΠΌΠ΅ΡΠ΅, Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ ΡΠΎΡ ΡΠ°Π½ΠΈΠ»ΠΈ ΡΠ²ΠΎΠΉ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π», Π½ΠΎ, ΠΊ ΡΠΎΠΆΠ°Π»Π΅Π½ΠΈΡ, Π²ΠΎΠΏΡΠΎΡ ΠΎ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΡΠΎΠ»ΠΈ Π Π Π² Π³Π΅Π½ΠΎΠΌΠ΅ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π΄ΠΎ ΡΠΈΡ ΠΏΠΎΡ ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ½ΡΠΌ. ΠΡΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ½ΡΠΌ, ΠΊΠ°ΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ ΡΡΠ°ΡΡΠ²ΡΡΡ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΡΠΊΠΎΠ»ΡΠΊΠΎ ΡΡΠΈΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΈ ΠΊΠ°ΠΊΠΈΠ΅ ΠΈΠΌΠ΅Π½Π½ΠΎ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°ΡΡΡΡ ΡΡΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ, ΠΊΠ°ΠΊ ΠΈ Π·Π° ΡΡΠ΅Ρ ΡΠ΅Π³ΠΎ ΠΏΡΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΡ Π²Π½Π΅ΡΠ½ΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΈΠ·ΠΌΠ΅Π½ΡΠ΅ΡΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ Π Π.
Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΌΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½Ρ Π΄Π»Ρ Π²ΡΡΡΠ½Π΅Π½ΠΈΡ Π²ΠΊΠ»Π°Π΄Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π Π Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ° Ρ ΠΎΠ·ΡΠΈΠ½Π°, ΠΈ ΠΏΡΠΈΠΌΠ΅Π½ΠΈΠ»ΠΈ ΠΈΡ Π΄Π»Ρ ΠΏΠΎΠ»Π½ΠΎΠ³Π΅Π½ΠΎΠΌΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ HERV ΠΈ LINE ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΈ Π΅Π΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΠΏΡΠΈ ΠΏΠ΅ΡΠ΅Ρ ΠΎΠ΄Π΅ ΠΎΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΡΠΊΠ°Π½Π΅ΠΉ ΠΊ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΠΌ.
ΠΠΠΠΠ 2. Π ΠΠΠ£ΠΠ¬Π’ΠΠ’Π« Π ΠΠΠ‘Π£ΠΠΠΠΠΠ.
2.1 ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° HERV-K.
ΠΡΠ±ΠΎΡ LTR Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ.
Π Π°Π½Π΅Π΅ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ LTR ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° HERV-K ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΎΡΠ½Π΅ΡΠ΅Π½Ρ ΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°ΠΌ, ΠΈΠ½ΡΠ΅Π³ΡΠΈΡΠΎΠ²Π°Π²ΡΠΈΠΌ Π² Π³Π΅Π½ΠΎΠΌ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΎΡ 50 Π΄ΠΎ 2 ΠΌΠ»Π½. Π»Π΅Ρ Π½Π°Π·Π°Π΄ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ [50]. ΠΠΎΠΏΡΠΎΡ ΠΎ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ LTR, ΠΎΡΠ½ΠΎΡΡΡΠΈΡ ΡΡ ΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ ΠΏΠΎΠ΄ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π°ΠΌ, ΠΎΡΡΠ°Π΅ΡΡΡ ΠΌΠ°Π»ΠΎΠΈΠ·ΡΡΠ΅Π½Π½ΡΠΌ. ΠΠ»Ρ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR, Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΠΈΡ ΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ Π²ΡΠ΅ΠΌΠ΅Π½Π΅ΠΌ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΈ Π² Π³Π΅Π½ΠΎΠΌ ΠΏΡΠΈΠΌΠ°ΡΠΎΠ², ΠΌΡ Π²ΡΠ±ΡΠ°Π»ΠΈ ΡΠ΅ΡΡΡΠ΅ LTR ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° HERV-K, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΡ Π½Π° ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΠ°Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ Π°Π½Π½ΠΎΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π² Π±Π°Π·Π΅ Π΄Π°Π½Π½ΡΡ GenBank (ΡΠ°Π±Π».2.1). ΠΡΠ±ΠΎΡ Π±ΡΠ» ΠΎΡΠ½ΠΎΠ²Π°Π½ Π½Π° Π΄Π²ΡΡ ΠΊΡΠΈΡΠ΅ΡΠΈΡΡ : 1) LTR Π΄ΠΎΠ»ΠΆΠ΅Π½ Π±ΡΡΡ ΠΏΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΡΠΌ ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΡ Π²ΡΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ- 2) LTR Π½Π΅ Π΄ΠΎΠ»ΠΆΠ΅Π½ Π½Π°Ρ ΠΎΠ΄ΠΈΡΡΡ Π² ΠΎΠΊΡΡΠΆΠ΅Π½ΠΈΠΈ ΠΏΠΎΠ²ΡΠΎΡΡΡΡΠΈΡ ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΡΡΠΎ Π΄Π°Π΅Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΏΠΎΠ΄ΠΎΠ±ΡΠ°ΡΡ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΡΠ΅ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΡ Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ. ΠΠ²Π° Π²ΡΠ±ΡΠ°Π½Π½ΡΡ LTR ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ ΡΠΎΠ»ΡΠΊΠΎ Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ ΡΠ²Π»ΡΠ»ΠΈΡΡ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΠΎ ΠΌΠΎΠ»ΠΎΠ΄ΡΠΌΠΈ, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ Π΄Π²Π° Π΄ΡΡΠ³ΠΈΡ ΡΠ²Π»ΡΠ»ΠΈΡΡ Π±ΠΎΠ»Π΅Π΅ ΡΡΠ°ΡΡΠΌΠΈ ΠΈ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ Π² Π³Π΅Π½ΠΎΠΌΠ°Ρ ΠΏΡΠΈΠΌΠ°ΡΠΎΠ².
ΠΠ°ΡΠ΅ΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ Π±ΡΠ»ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡ Π»ΠΈ ΠΎΡΠΎΠ±ΡΠ°Π½Π½ΡΠ΅ Π½Π°ΠΌΠΈ LTR ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ, ΡΠ²Π»ΡΠ΅ΡΡΡ Π»ΠΈ ΡΡΠ° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠΊΠ°Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΠΉ ΠΈ ΠΈΠ·ΠΌΠ΅Π½ΡΠ΅ΡΡΡ Π»ΠΈ ΠΎΠ½Π° ΠΏΠΎΠ΄ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ Π²Π½Π΅ΡΠ½ΠΈΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ². Π Π°Π½Π΅Π΅ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΠ΅ LTRs ΠΌΠΎΠ³ΡΡ Π²ΡΡΡΡΠΏΠ°ΡΡ Π² ΡΠΎΠ»ΠΈ ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ°, ΡΠ½Ρ Π°Π½ΡΠ΅ΡΠ° ΠΈ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ, ΠΈΠ»ΠΈ ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ Π²Ρ ΠΎΠ΄ΠΈΡΡ Π² ΡΠΎΡΡΠ°Π² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠ° [60, 85, 86]. ΠΡΡΠ°Π΅ΡΡΡ Π½Π΅ΠΈΠ·Π²Π΅ΡΡΠ½ΡΠΌ, ΠΌΠΎΠΆΠ΅Ρ Π»ΠΈ ΠΎΠ΄ΠΈΠ½ ΠΈ ΡΠΎΡ ΠΆΠ΅ LTR ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π² ΠΎΠ΄Π½ΠΎΠΉ ΠΈ ΡΠΎΠΉ ΠΆΠ΅ ΡΠΊΠ°Π½ΠΈ ΠΈΠ»ΠΈ Π² ΡΠ°Π·Π½ΡΡ ΡΠΊΠ°Π½ΡΡ ΠΏΡΠΈ ΡΠ°Π·Π½ΡΡ Π²Π½Π΅ΡΠ½ΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΡΡ . Π§ΡΠΎΠ±Ρ ΠΎΡΠ΅Π½ΠΈΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR, Π±ΡΠ»Π° ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΡ Π΅ΠΌΠ° ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½Π°Ρ Π½Π° ΡΠΈΡ. 2.1.
HERV-K LTR.
ΠΠ΅Π½ΠΎΠΌΠ½Π°Ρ ΠΠΠ ΠΠ΅Π½ΠΎΠΌΠ½Π°Ρ ΠΠΠ — ΠΈΠ· | r | us |J.
Π ΠΠ I.
I ΡΠ΅ΠΏΡ ΠΊΠΠΠ.
ΠΠ½ΠΈΡΠΈΠ°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ.
I R I U5 |.
Π’ΡΡΠΌΠΈΠ½Π°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΈΠ· I R.
Π’ΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΡΠΊΠ²ΠΎΠ·Ρ LTR ΠΈΠ· | R | LJ5 h.
PI Π 2 Π Π Π 4.
U3 PCR ΠΏΡΠΎΠ΄Ρ1ΡΠ³ U5 PCR ΠΏΡΠΎΠ΄ΡΡΡ.
ΠΠ½ΠΈΡΠΈΠ°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ +.
Π’Π΅ΡΠΌΠΈΠΏΠ°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ.
Π’ΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΡΠΊΠ²ΠΎΠ·Ρ LTR +.
Π ΠΈΡ. 2.1 Π‘Ρ Π΅ΠΌΠ° Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ RT-PCR. ΠΠ°ΡΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π 1/Π 2 ΠΈ Π Π/Π 4 ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ U3 ΠΈ U5 ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ LTR HERV-K, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΠ°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ LTR ΠΈ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΡ RT-PCR ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½Ρ Π² Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ°ΡΡΠΈ ΡΠΈΡΡΠ½ΠΊΠ°.
ΠΡΠ»ΠΈ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΡΡΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΡΠ°ΡΡΠΈΡ LTR Π² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ: 1) LTR ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΡΠΎΠΌΠΎΡΠΎΡΠΎΠΌ ΠΈ ΠΈΠ½ΠΈΡΠΈΠΈΡΡΠ΅Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ- 2) LTR ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΡΠ΅Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ, ΠΈΠ½ΠΈΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Ρ Π²Π½Π΅ΡΠ½Π΅Π³ΠΎ ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ°- 3) LTR Π²Ρ ΠΎΠ΄ΠΈΡ Π² ΡΠΎΡΡΠ°Π² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠ°, ΠΈΠ½ΠΈΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Ρ Π΄ΡΡΠ³ΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ². ΠΡΠ½Π΅ΡΠ΅Π½ΠΈΠ΅ Π΄Π°Π½Π½ΠΎΠ³ΠΎ LTR ΠΊ ΡΠΎΠΉ ΠΈΠ»ΠΈ ΠΈΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ RT-PCR Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π΄Π²ΡΡ ΠΏΠ°Ρ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ². ΠΠ΅ΡΠ²Π°Ρ ΠΏΠ°ΡΠ° ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π²ΠΊΠ»ΡΡΠ°Π»Π° Π² ΡΠ΅Π±Ρ ΠΏΡΠ°ΠΉΠΌΠ΅Ρ, ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΡΠ½ΡΠΉ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ°ΡΡΠΈ Π³Π΅Π½ΠΎΠΌΠ°, ΡΠ»Π°Π½ΠΊΠΈΡΡΡΡΠ΅ΠΉ U3 ΠΎΠ±Π»Π°ΡΡΡ LTR ΠΈ ΠΏΡΠ°ΠΉΠΌΠ΅Ρ, ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΡΠ½ΡΠΉ U3 ΠΎΠ±Π»Π°ΡΡΠΈ LTR (ΠΏΡΠ°ΠΉΠΌΠ΅ΡΡ Π 1 ΠΈ Π 2 Π½Π° ΡΠΈΡ. 2.1).
ΠΡΠΎΡΠ°Ρ ΠΏΠ°ΡΠ° ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² ΡΠΎΡΡΠΎΡΠ»Π° ΠΈΠ· ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°, ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠΌΡ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΌΡ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΡ, ΡΠ»Π°Π½ΠΊΠΈΡΡΡΡΠ΅ΠΌΡ U5 ΠΎΠ±Π»Π°ΡΡΡ LTR ΠΈ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΡΠ°ΠΌΠΎΠΉ U5 ΠΎΠ±Π»Π°ΡΡΠΈ LTR (ΠΏΡΠ°ΠΉΠΌΠ΅ΡΡ Π Π ΠΈ Π 4 Π½Π° ΡΠΈΡ. 2.1). ΠΡΠ»ΠΈ LTR, Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ½ΠΎ LTR ΡΠ΅ΡΡΠΎΠ²ΠΈΡΡΡΠΎΠ², ΠΈΠ½ΠΈΡΠΈΠΈΡΡΠ΅Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ Π½Π° Π³ΡΠ°Π½ΠΈΡΠ΅ U3 ΠΈ R ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ, ΡΠΎ ΠΏΡΠΈ RT-PCR Ρ ΠΏΡΠ°ΠΉΠΌ Π΅ΡΠ°ΠΌΠΈ Π Π ΠΈ Π 4 Π±ΡΠ΄Π΅Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²ΡΠ²Π°ΡΡΡΡ ΠΏΡΠΎΠ΄ΡΠΊΡ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ, Π° Ρ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°ΠΌΠΈ Π 1 ΠΈ Π 2 — Π½Π΅Ρ. (Π‘ΡΡΠΎΠ³ΠΎ Π³ΠΎΠ²ΠΎΡΡ, ΡΡΠΎ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ ΡΠ»ΡΡΠ°Ρ, ΠΊΠΎΠ³Π΄Π° ΠΈΠ½ΠΈΡΠΈΠ°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΠΈΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ LTR ΠΌΠ΅ΠΆΠ΄Ρ ΡΠΊΠ°Π·Π°Π½Π½ΡΠΌΠΈ Π΄Π²ΡΠΌΡ ΠΏΠ°ΡΠ°ΠΌΠΈ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ²). ΠΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Ρ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°ΠΌΠΈ Π 1 ΠΈ Π 2, Π½ΠΎ Π½Π΅ Ρ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°ΠΌΠΈ Π Π ΠΈ Π 4, Π±ΡΠ΄Π΅Ρ ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΠΈΡΡ ΡΠΎΠ»ΡΠΊΠΎ ΡΠΎΠ³Π΄Π°, ΠΊΠΎΠ³Π΄Π° LTR ΡΠ»ΡΠΆΠΈΡ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠΎΠΌ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ. ΠΠ±Π΅ ΠΏΠ°ΡΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π±ΡΠ΄ΡΡ Π΄Π°Π²Π°ΡΡ ΠΏΡΠΎΠ΄ΡΠΊΡΡ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π² ΡΠΎΠΌ ΡΠ»ΡΡΠ°Π΅, Π΅ΡΠ»ΠΈ LTR Π²Ρ ΠΎΠ΄ΠΈΡ Π² ΡΠΎΡΡΠ°Π² Π±ΠΎΠ»Π΅Π΅ Π΄Π»ΠΈΠ½Π½ΠΎΠ³ΠΎ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠ°. ΠΠ»Ρ ΡΠ°Π·Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR Π±ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π½Π°Π±ΠΎΡΡ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΡΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π 1 ΠΈ Π 4 (ΡΠ°Π±Π». 2, Π³Π»Π°Π²Π° «ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ»).
ΠΡΠΈ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ U3 ΠΈ U5 ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ LTR, ΠΌΡ ΡΡΠΈΡΠ°Π»ΠΈ, ΡΡΠΎ ΡΡΠ°Π³ΠΌΠ΅Π½Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠ±ΠΈΡΡΠ΅ΡΡΡ («+» Π² ΡΠ°Π±Π». 2.1), Π΅ΡΠ»ΠΈ ΠΊ 37 ΡΠΈΠΊΠ»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π±ΡΠ»ΠΎ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΊΠ°ΠΊ ΠΌΠΈΠ½ΠΈΠΌΡΠΌ 100 Π½Π³ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°. Π Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π΅ ΡΠ»ΡΡΠ°Π΅Π² ΠΌΡ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ 100 Π½Π³ ΠΏΡΠΎΠ΄ΡΠΊΡΠ° ΡΠΆΠ΅ ΠΏΠΎΡΠ»Π΅ 35 ΡΠΈΠΊΠ»ΠΎΠ² Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΈΠ»ΠΈ ΡΠ°Π½Π΅Π΅. ΠΡ ΡΡΠΈΡΠ°Π»ΠΈ, ΡΡΠΎ ΡΡΠ°Π³ΠΌΠ΅Π½Ρ Π½Π΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠ±ΠΈΡΡΠ΅ΡΡΡ, Π΅ΡΠ»ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡΠ° Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π±ΡΠ»ΠΎ Π½ΠΈΠΆΠ΅ ΡΡΠΎΠ²Π½Ρ Π΄Π΅ΡΠ΅ΠΊΡΠΈΠΈ ΠΈΠ»ΠΈ ΡΠΎΡΡΠ°Π²Π»ΡΠ»ΠΎ ΠΌΠ΅Π½Π΅Π΅ 5% ΠΎΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠ°ΠΌΠΎΠ³ΠΎ ΡΠ»Π°Π±ΠΎΠ³ΠΎ ΠΈΠ· Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΡΠ΅ΠΌΡΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² («-» Π² ΡΠ°Π±Π». ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ²).
Π‘ΠΈΠ½ΡΠ΅Π· ΠΏΠ΅ΡΠ²ΠΎΠΉ ΡΠ΅ΠΏΠΈ ΠΊΠΠΠ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° ΠΌΠ°ΡΡΠΈΡΠ΅ ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΉ Π ΠΠ, Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΠΎΠΉ ΠΈΠ· ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΠΊΠ°Π½Π΅ΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΠΉ. ΠΠ»Ρ ΠΈΠ·Π±Π΅ΠΆΠ°Π½ΠΈΡ ΠΏΠΎΡΠ΅ΡΡ Π½Π΅-ΠΏΠΎΠ»ΠΈΠ°Π΄Π΅Π½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ LTR-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² ΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ², Π² ΠΊΠΎΡΠΎΡΡΡ LTR Π²Ρ ΠΎΠ΄ΠΈΡ Π² ΡΠΎΡΡΠ°Π² ΠΈΠ½ΡΡΠΎΠ½ΠΎΠ², ΠΊΠΠΠ Π±ΡΠ»Π° ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΌΠ΅ΡΠΈ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ²: oligo (dT) ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ° ΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈΡ Π³Π΅ΠΊΡΠ°ΠΌΠ΅ΡΠΎΠ². ΠΠ΄Π½Π°ΠΊΠΎ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ RT-PCR Π°Π½Π°Π»ΠΈΠ·Π° Π±ΡΠ»ΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΡΠΌΠΈ, ΠΊΠΎΠ³Π΄Π° Π΄Π»Ρ ΡΠΈΠ½ΡΠ΅Π·Π° Π±ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΡΠΎΠ»ΡΠΊΠΎ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π³Π΅ΠΊΡΠ°ΠΌΠ΅ΡΡ (Π΄Π°Π½Π½ΡΠ΅ Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΡΡ). Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ΅ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ, ΠΏΡΠΈΠ³ΠΎΡΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ Π°Π±ΡΠΎΠ»ΡΡΠ½ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½ΠΎ ΠΎΠΏΡΡΠ½ΡΠΌ, Π·Π° ΠΈΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΎΡΡΡΡΡΡΠ²ΠΈΡ Π² ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΌΠ΅ΡΠΈ ΠΎΠ±ΡΠ°ΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠ°Π·Ρ.
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π΄Π²ΡΡ LTR ΠΈΠ· Π»ΠΎΠΊΡΡΠ° ΠΠ64 ΠΈ ΠΠ‘52 374 ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ Π½Π° ΡΠΈΡ. 2.2, Π.
ΠΠ»Ρ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΈΡ ΡΠΎΠ³ΠΎ, ΡΡΠΎ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΡΠ΅ Π³Π΅Π½ΠΎΠΌΠ½ΡΠ΅ Π»ΠΎΠΊΡΡΡ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ Π² Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ Π²ΡΠ΅Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΡ ΡΠΊΠ°Π½Π΅ΠΉ ΠΈ Π»ΠΈΠ½ΠΈΠΉ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΌΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ PCR Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ Ρ ΠΏΠ°ΡΠΎΠΉ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π 1 ΠΈ Π 4 (Π΄Π°Π½Π½ΡΠ΅ Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΡΡ). PCR ΠΏΡΠΎΠ΄ΡΠΊΡ ΠΎΡΡΡΡΡΡΠ²ΠΎΠ²Π°Π» ΠΏΡΠΈ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Ρ ΡΡΠΎΠΉ ΠΏΠ°ΡΠΎΠΉ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ ΠΈΠ»ΠΈ ΠΊΠΠΠ Π³ΡΡΠ·ΡΠ½ΠΎΠ², Π½Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΊΠ°ΠΊΠΈΡ -Π»ΠΈΠ±ΠΎ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ HERV.
Π§ΡΠΎΠ±Ρ ΠΎΡΠ΅Π½ΠΈΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ LTR ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ LTR-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ², ΠΌΠΎΠΆΠ½ΠΎ ΡΠΎΠΎΡΠ½Π΅ΡΡΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Ρ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΠΌ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π°ΠΊΡΠΈΠ½Π°. ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ LTR-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² ΡΠΎΡΡΠ°Π²Π»ΡΠ»ΠΎ ΠΎΠΊΠΎΠ»ΠΎ 0.1−1% ΠΎΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π°ΠΊΡΠΈΠ½Π° Π² ΠΎΠ΄Π½ΠΈΡ ΠΈ ΡΠ΅Ρ ΠΆΠ΅ ΡΠΊΠ°Π½ΡΡ ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΡΡ . ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎ Π½ΠΈΠ·ΠΊΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΡ, ΡΡΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΡ Π±ΡΠ»ΠΈ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½Ρ ΠΊ 33−37 ΡΠΈΠΊΠ»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ.
Π’ΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ LTR Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΠΊΠ°Π½ΡΡ .
ΠΠ°ΠΊ Π²ΠΈΠ΄Π½ΠΎ ΠΈΠ· ΡΠΈΡ. 2.2 Π, ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ U5 ΠΎΠ±Π»Π°ΡΡΡ LTR ΠΈΠ· Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠ³ΠΎ Π»ΠΎΠΊΡΡΠ° ΠΠ6 684 ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π» Π² ΡΠ΅ΠΌΠΈΠ½ΠΎΠΌΠ΅, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ U3 ΠΎΠ±Π»Π°ΡΡΡ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π» Π² ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ΅.
Π Π.
ΠΠ6 684.
U3 (Π 1- Π 2) U5 (Π Π — Π 4).
Π‘Π΅ΠΌΠΈΠ½ΠΎΠΌΠ°.
ΠΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ° Ρ.
ΠΠ‘52 374.
Π‘Π΅ΠΌΠΈΠ½ΠΎΠΌΠ°.
ΠΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ°.
33 35 37.
35 37.
ΠΠ‘2 508 ΠΠ»Π΅ΡΠΎΡΠ½Π°Ρ Π»ΠΈΠ½ΠΈΡ Jurkat ΠΏΠΎΡΠ»Π΅ ΡΠ΅ΠΏΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΎΠΊΠ°.
U3 (Π 1- Π 2) U5 (Π ΠΠ 4).
33 35 37 33 35 37 ΠΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ LTR (Π 1- Π 4).
34 37 34 37 ΠΊΠΠΠ Π³Π΅Π½ΠΎΠΌΠ½Π°Ρ ΠΠΠ.
Π ΠΈΡ. 2.2 ΠΡΠΈΠΌΠ΅Ρ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ RT-PCR. ΠΠ° ΠΏΠ°Π½Π΅Π»ΠΈ (Π) ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΠΏΡΡ ΠΎΠ»ΡΡ . ΠΠ°Π΄ΠΏΠΈΡΠΈ U3 ΠΈ U5 ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Ρ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°ΠΌΠΈ, ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΡΠ½ΡΠΌΠΈ U3 ΠΈ U5 ΠΎΠ±Π»Π°ΡΡΡΠΌ LTR, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠ6 684, ΠΠ‘52 374, ΠΠ‘2 508 — Π½ΠΎΠΌΠ΅ΡΠ° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ LTR-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ Π³Π΅Π½ΠΎΠΌΠ½ΡΡ Π»ΠΎΠΊΡΡΠΎΠ² ΠΏΠΎ GenBank. Π¦ΠΈΡΡΡ 33−35−37 ΠΎΠ±ΠΎΠ·Π½Π°ΡΠ°ΡΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΠΈΠΊΠ»ΠΎΠ² Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ. ΠΠ° ΠΏΠ°Π½Π΅Π»ΠΈ (Π) ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ U3 ΠΈ U5 ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ LTR ΠΠ‘2 508 Π² Π»ΠΈΠ½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Jurkat ΠΏΠΎΡΠ»Π΅ ΡΠ΅ΠΏΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΎΠΊΠ°. ΠΡΡΡΡΡΡΠ²ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠ°, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠ΅Π³ΠΎ ΠΏΠΎΠ»Π½ΠΎΡΠ°Π·ΠΌΠ΅ΡΠ½ΡΠΉ LTR ΠΠ‘2 508, Π΄ΠΎΠΊΠ°Π·Π°Π½ΠΎ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠ΅ΠΉ Ρ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠ°ΠΌΠΈ PI-P4.
ΠΠ°ΠΎΠ±ΠΎΡΠΎΡ, ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ U3 ΠΎΠ±Π»Π°ΡΡΡ LTR ΠΈΠ· Π»ΠΎΠΊΡΡΠ° ΠΠ‘52 374 Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π² ΡΠ΅ΠΌΠΈΠ½ΠΎΠΌΠ΅ ΠΈ ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ΅, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ U5 ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ ΡΡΠΎΠ³ΠΎ LTR Π½Π΅ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½Π΅ΠΉ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ LTR Π½Π° ΠΏΠ°Π½Π΅Π»ΠΈ ΠΊΠΠΠ ΠΈΠ· ΡΠ΅ΡΡΠΈ ΡΠΊΠ°Π½Π΅ΠΉ ΠΈ ΡΡΠ΅Ρ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΠΉ ΡΡΠΌΠΌΠΈΡΠΎΠ²Π°Π½Ρ Π² ΡΠ°Π±Π»ΠΈΡΠ΅ 2.1. ΠΠ· ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΌΠΎΠΆΠ½ΠΎ ΡΠ΄Π΅Π»Π°ΡΡ Π²ΡΠ²ΠΎΠ΄, ΡΡΠΎ Π² ΡΠ΅Π»ΠΎΠΌ, ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ LTR Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΠΈΠΏΠ° ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΈΠ»ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ. ΠΠ°ΠΏΡΠΈΠΌΠ΅Ρ, LTR-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ ΠΈΠ· Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠ³ΠΎ Π»ΠΎΠΊΡΡΠ° ΠΠ‘2 508 Π½Π΅ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π² ΠΏΠ»Π°ΡΠ΅Π½ΡΠ΅ ΠΈ ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ΅, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ U3 ΠΈ U5 ΠΎΠ±Π»Π°ΡΡΠΈ ΡΡΠΎΠ³ΠΎ LTR ΡΡΠ°Π½ΡΠΊΡΠΈΠ±ΠΈΡΠΎΠ²Π°Π»ΠΈΡΡ Π² ΡΠ΅ΠΌΠΈΠ½ΠΎΠΌΠ΅ ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ ΡΠ°Π±Π΄ΠΎΠΌΠΈΠΎΡΠ°ΡΠΊΠΎΠΌΡ. ΠΠΎΠΌΠΈΠΌΠΎ ΡΡΠΎΠ³ΠΎ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΠΎΡΠΌΠ΅ΡΠΈΡΡ, ΡΡΠΎ ΡΠΏΠ΅ΠΊΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR ΠΎΡΠ»ΠΈΡΠ°Π»ΡΡ Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΡΡ , ΠΈ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ (ΡΠ°Π±Π». 2.1).
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- ΠΠ²ΡΠΎΡ Π²ΡΡΠ°ΠΆΠ°Π΅Ρ Π³Π»ΡΠ±ΠΎΠΊΡΡ ΠΏΡΠΈΠ·Π½Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΈ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ Π·Π° ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΡΠ²ΠΎΡΡΠ΅ΡΠΊΠΎΠΉ Π°ΡΠΌΠΎΡΡΠ΅ΡΡ, Π·Π° ΠΏΠΎΠΌΠΎΡΡ, ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΡ ΠΈ Π΄ΠΎΠ±ΡΠΎΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ Π²ΡΠ΅ΠΌ ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠ°ΠΌ ΠΈ Π°ΡΠΏΠΈΡΠ°Π½ΡΠ°ΠΌ ΠΠ°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π‘ΡΡΡΠΊΡΡΡΡ ΠΈ Π€ΡΠ½ΠΊΡΠΈΠΉ ΠΠ΅Π½ΠΎΠ² Π§Π΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΠΠ₯ Π ΠΠ.
- ΠΠ²ΡΠΎΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ΅Π½ Π. Π. ΠΠΎΡΠ°ΠΏΠΎΠ²Ρ ΠΈ Π. Π. Π‘ΠΊΠ°ΠΏΡΠΎΠ²ΠΎΠΉ Π·Π° ΡΠΎΡΡΡΠ΄Π½ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΈ ΡΠΈΠ½ΡΠ΅Π· ΠΎΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ², Π±Π΅Π· ΠΊΠΎΡΠΎΡΡΡ Π΄Π°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° Π±ΡΠ»Π° Π±Ρ Π½Π΅Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Π°.
- ΠΠ²ΡΠΎΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ΅Π½ Π‘Π΅Π½ΡΡΠ΅ ΠΠ°ΡΠ°Π»ΡΠ΅ ΠΠΎΡΠΈΡΠΎΠ²Π½Π΅ ΠΈ ΠΠ»Π΅ΠΉΠΌΠ°Π½ ΠΠ½Π½Π΅ Π·Π° ΡΠΎΡΡΡΠ΄Π½ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΈ ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ Π΄Π»Ρ ΡΠ°Π±ΠΎΡΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ.