Молекулярная характеристика герминальной гранулы, участвующей в репрессии повторов Stellate в семенниках Drosophila melanogaster

6. Выводы.

1. В сперматоцитах Drosophila melanogaster среди множества мелких околоядерных гранул nuage, обнаружена крупная терминальная гранула (одна на клетку). Гранула обогащена белками, функционирующими в piPHK-пути (Vasa, Aubergine, AG03, Tudor, Spindle-E, Squash, Cutoff) и miPHK-пути сайленсинга (Belle и AGOl), а также содержит белок DCP1 — компонент системы деградации мРНК;

2. Крупная гранула состоит из нескольких периферийных долей, расположенных вокруг центральной доли. Белки Aubergine, Vasa, Tudor, Spindle-E, Squash, Cutoff, AGOl и DCP1 обнаружены в составе внешних долей, тогда как белок AG03 располагается в центре гранулы;

3. Дерепрессия тандемных повторов Stellate в результате нарушения образования piPHK на фоне мутаций в генах vasa, aub и ago3 ассоциирована с исчезновением крупных гранул в первичных сперматоцитах;

4. В отсутствие симметричного метилирования остатков аргинина в белке подсемейства PIWI Aubergine при мутации в гене аргининметилтрансферазы capsuleen наблюдается дерепрессия повторов Stellate, нарушение образования piPHK Su (Ste) и исчезновение крупных терминальных гранул.

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