ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ Π½Π΅ΠΉΡΠΎΠΌΡΡΠ΅ΡΠ½ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
ΠΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π° ΡΠ ΠΠΠΠΈΠ· ΡΠ½ΠΈΠΆΠ°ΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΅Π΅ Π»ΠΈΠ·ΠΈΠ½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ, Π½ΠΎ Π½Π΅ ΠΏΡΠ΅ΠΏΡΡΡΡΠ²ΡΡΡ ΠΈΠΌΠΏΠΎΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ. ΠΠ΅ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΠ΅ Π²Π΅ΡΡΠΈΠΈ ΡΠ ΠΠΠ»ΠΈΠ· ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π² Π΄Π΅Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΈΠ»ΠΈ ΠΌΠΈΡΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΠ΅. Π¦Π΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΏΠΎΡΡΠ°… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- Π‘ΠΏΠΈΡΠΎΠΊ ΡΠΎΠΊΡΠ°ΡΠ΅Π½ΠΈΠΉ
- ΠΠ±Π·ΠΎΡ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
- 1. ΠΠΌΠΏΠΎΡΡ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ
- 1. 2. ΠΡΠ΅ΡΡΠ°Π½ΡΠ»ΠΎΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΡΠ°Π·Π²ΠΎΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² ΠΌΠΈΡΠΎ-Ρ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² ΠΈ ΠΈΡ ΡΡΠ°Π½ΡΠΏΠΎΡΡ ΠΊ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ
- 1. 3. Π’ΡΠ°Π½ΡΠ»ΠΎΠΊΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ Π²Π½Π΅ΡΠ½Π΅ΠΉ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ
- 1. 4. Π’ΡΠ°Π½ΡΠ»ΠΎΠΊΠ°Π·Ρ Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ
- 1. 4. 1. ΠΠΌΠΏΠΎΡΡ Π±Π΅Π»ΠΊΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΠΊΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΡΡ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ
- 1. 4. 2. ΠΠΌΠΏΠΎΡΡ Π³ΠΈΠ΄ΡΠΎΡΠΎΠ±Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Ρ Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΠΌΠΈ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΡΠΌΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΠΌΠΈ
- 1. 4. 3. ΠΠ₯Π-ΡΡΠ°Π½ΡΠ»ΠΎΠΊΠ°Π·Π°
- 2. 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ
- 2. 1. 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Tetrahymena
- 2. 1. 2. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΡΠΈΠΏΠ°Π½ΠΎΡΠΎΠΌΠ°ΡΠΈΠ΄
- 2. 2. ΠΠΌΠΏΠΎΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ
- 2. 3. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 2. 4. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
- 3. 1. ΠΠΎΠ»Π΅Π·Π½ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΠΌΡΡΠ°ΡΠΈΡΠΌΠΈ Π² Π³Π΅Π½Π°Ρ ΡΠ ΠΠ
- 1. 1. Π¨ΡΠ°ΠΌΠΌΡ Escherichia col
- 1. 2. Π¨ΡΠ°ΠΌΠΌΡ Saccharomyces cerevisiae
- 1. 3. ΠΠΈΠ½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 3. 1. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π· (Kunkel et al., 1987)
- 3. 1. 1. ΠΡΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°ΡΠΈΠ»-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΡΠ°Π³ΠΎΠ²
- 3. 1. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΠΎΠΉ ΠΠΠ ΠΈΠ· ΡΠ°Π³ΠΎΠ²ΡΡ ΡΠ°ΡΡΠΈΡ
- 3. 1. 3. Π€ΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ²
- 3. 1. 4. ΠΡΠΆΠΈΠ³ ΠΎΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² Π½Π° ΠΌΠ°ΡΡΠΈΡΡ
- 3. 1. 5. Π‘ΠΈΠ½ΡΠ΅Π· ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΡΠ½ΠΎΠΉ ΡΠ΅ΠΏΠΈ ΠΠΠ
- 3. 2. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ E. col
- 3. 2. 1. ΠΠ΅ΡΠΎΠ΄ ΡΠΎΠ»Π΅Π²ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ
- 3. 2. 2. ΠΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΡ
- 3. 3. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΎΡΠΈΡΡΠΊΠ° ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΡΡ ΠΠΠ ΡΠ°Π³ΠΎΠ² Π΄Π»Ρ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ
- 3. 4. Π‘Π΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΡΡ ΠΈ Π΄Π²ΡΠ½ΠΈΡΠ΅Π²ΡΡ ΠΠΠ
- 3. 5. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ΅ΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠΎΡΠΌΡ ΠΠΠ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΡ ΡΠ°Π³ΠΎΠ² ΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΡΡ ΠΠΠ
- 3. 6. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠ°ΡΡΠΈΡ Π΄Π»Ρ Π’7-ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ PCR
- 3. 6. 1. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ
- 3. 6. 2. Π Π°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΠΠ ΡΠ΅ΡΡΡΠΈΠΊΡΠ°Π·ΠΎΠΉ BstNl
- 3. 7. Π’7- ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ
- 3. 8. ΠΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π’7-ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π³Π΅Π½Π°ΡΠ ΠΠ
- 3. 9. Π Π°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΌΠ΅ΡΠ΅Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ²
- 3. 10. Π‘Π²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ Π ΠΠ ΠΈ Π’7-ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Ρ pre-MSK
- 3. 11. Π’ΡΠ°Π½ΡΠΏΠΎΡΡ ΡΠ°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎ ΠΌΠ΅ΡΠ΅Π½Π½ΡΡ
ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π³Π΅Π½Π° ΡΠ ΠΠ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ
- 3. 11. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ
- 3. 11. 2. ΠΠΌΠΏΠΎΡΡ Π ΠΠ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ
- 3. 12. ΠΠΌΠΏΠΎΡΡ 358-ΠΌΠ΅ΡΠ΅Π½Π½ΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 3. 13. ΠΠΌΠΌΡΠ½ΠΎΠΏΡΠ΅ΡΠΈΠΏΠΈΡΠ°ΡΠΈΡ
- 3. 14. Western-Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ
- 3. 15. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 3. 16. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠΌΠΌΠ°ΡΠ½ΡΡ ΠΈ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π ΠΠ
- 3. 17. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»-ΡΠ ΠΠ Π² ΠΊΠΈΡΠ»ΡΡ ΡΡΠ»ΠΎΠ²ΠΈΡΡ
- 3. 18. ΠΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ ΠΏΠΎ ΠΠΎΠ·Π΅ΡΠ½Ρ ΠΈ ΡΠΎΡΠ΅ΡΠ½Π°Ρ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ
- 3. 19. ΠΠ±ΡΠ°ΡΠ½Π°Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΠΈ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ
- 3. 20. ΠΠΌΠΏΠΎΡΡ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ
ΡΠ ΠΠ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 3. 20. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 3. 20. 2. ΠΠΌΠΏΠΎΡΡ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ ΡΠ ΠΠ
- 3. 21. Π’ΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² ΠΊΡΠ»ΡΡΡΡΠ΅
- 3. 22. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΠ΅Π΄Π΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Π·Π²Π΅Π΄Π΅Π½ΠΈΡ
- 3. 23. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΈ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ ΠΠ ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 3. 24. ΠΠΈΡΡΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΈΡΠΎΡ ΡΠΎΠΌ Π‘ ΠΎΠΊΡΠΈΠ΄Π°Π·Ρ
- 3. 25. ΠΡΠ΅Π½ΠΊΠ° ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΏΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΡ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π° ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ
- 3. 26. ΠΠΈΠΎΡΠΈΠ½ΡΠ΅Π· Π±Π΅Π»ΠΊΠ° Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² ΠΊΡΠ»ΡΡΡΡΠ΅
- 1. 1. ΠΡΡΠ°ΡΠΈΠΈ Π² Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π΅ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π²Π»ΠΈΡΡΡ Π½Π° Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ ΠΠ
- 1. 2. ΠΡΡΠ°ΡΠΈΠΈ Π² Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π΅ ΡΠ ΠΠ1 ΠΏΠΎ-ΡΠ°Π·Π½ΠΎΠΌΡ Π²Π»ΠΈΡΡΡ Π½Π° ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Ρ npe-MSK, Π½ΠΎ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡΡΡ Π½Π° ΠΈΡ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ
- 2. 1. ΠΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ ΡΠ ΠΠΠΠΈΠ· ΠΌΠΎΠ³ΡΡ ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π² ΠΌΠΈΡΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΠ΅
- 2. 2. ΡΠ ΠΠ, ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΠ΅ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ, ΠΌΠΎΠ³ΡΡ ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ°
- 2. 3. ΠΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΠ΅ ΡΠ ΠΠ ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ° in vivo ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΡΡΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π½ΠΎΠ½ΡΠ΅Π½Ρ ΠΌΡΡΠ°ΡΠΈΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ
- 2. 4. ΠΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΠ΅ in vivo ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ ΡΠ ΠΠ2 ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΡΡΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π½ΠΎΠ½ΡΠ΅Π½Ρ ΠΌΡΡΠ°ΡΠΈΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ
- 3. 1. ΠΡΠΎΠΆΠΆΠ΅Π²ΡΠ΅ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΠ΅ ΡΠ ΠΠ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° in vitro
- 3. 2. ΠΡΠΎΠΆΠΆΠ΅Π²ΡΠ΅ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΠ΅ ΡΠ ΠΠ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° in vivo
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ Π½Π΅ΠΉΡΠΎΠΌΡΡΠ΅ΡΠ½ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ — ΡΡΠΎ ΠΎΡΠ³Π°Π½Π΅Π»Π»Ρ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠ½Π΅ΡΠ³ΠΈΠ΅ΠΉ Π·Π° ΡΡΠ΅Ρ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ (Saraste, 1999). ΠΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΈΠΌΠ΅ΡΡ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΡ ΠΠΠ ΠΈ ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° Π±Π΅Π»ΠΊΠ°. ΠΠ΄Π½Π°ΠΊΠΎ Π±ΠΎΠ»ΡΡΠ°Ρ ΡΠ°ΡΡΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Π·Π°ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½Π° Π² ΡΠ΄Π΅ΡΠ½ΠΎΠΌ Π³Π΅Π½ΠΎΠΌΠ΅ ΠΈ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ ΠΈΠ· ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΡ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄Π»Ρ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΡΡΡΠΊΡΡΡΠ½ΡΠ΅ Π ΠΠ, Π·Π°ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π² ΡΠ΄ΡΠ΅, ΡΠ°ΠΊΠΆΠ΅ Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΡ Π΄Π»Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° Π±Π΅Π»ΠΊΠ°. ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ° ΡΠ ΠΠ ΡΠ΅ΡΠ΅Π· ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΡΡΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΡΠΈΠ»ΡΠ½ΠΎ Π²Π°ΡΡΠΈΡΡΡΡ ΠΎΡ Π²ΠΈΠ΄Π° ΠΊ Π²ΠΈΠ΄Ρ. Π’Π°ΠΊ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ, Ρ ΡΡΠΈΠΏΠ°Π½ΠΎΡΠΎΠΌΠ°ΡΠΈΠ΄ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΡΡΡΡ Π²ΡΠ΅ ΡΠ ΠΠ, Π° Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΏΠ΅ΠΊΠ°ΡΡΠΊΠΈΡ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Saccharomyces cerevisiae ΡΠΎΠ»ΡΠΊΠΎ ΠΎΠ΄Π½Π° — ΠΎΠ΄Π½Π° ΠΈΠ· Π΄Π²ΡΡ ΠΈΠ·ΠΎΠ°ΠΊΡΠ΅ΠΏΡΠΎΡΠ½ΡΡ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΡ ΡΠ ΠΠ (Schneider and Marechal-Drouard, 2000). Π ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ ΡΠ ΠΠ Π½Π΅ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ, ΠΎΠ΄Π½Π°ΠΊΠΎ Π² ΡΡΠΈ ΠΎΡΠ³Π°Π½Π΅Π»Π»Ρ ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡ Π ΠΠ, Π²Ρ ΠΎΠ΄ΡΡΠΈΠ΅ Π² ΡΠΎΡΡΠ°Π² Π ΠΠΠ°Π·ΡΠ ΠΈ MRP, Π° ΡΠ°ΠΊΠΆΠ΅ 5S ΡΠ ΠΠ.
ΠΠΎΠ»ΡΡΠ°Ρ ΡΠ°ΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ ΠΠ Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΠ° Π΄Π»Ρ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ. Π Π²ΠΎΡ ΡΠΎΠ»Ρ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ½Π°. ΠΠ½Π° Π½Π΅ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΡΠ΅ΡΡΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π»ΠΈΠ·ΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π·ΠΎΠΉ, Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ΅Π³ΠΎ Π±ΡΠ»ΠΎ Π²ΡΡΠΊΠ°Π·Π°Π½ΠΎ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ Π΄Π°Π½Π½Π°Ρ ΡΠ ΠΠ Π½Π΅ ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π΅Ρ ΡΡΠ°ΡΡΠΈΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ° (Martin et al., 1979). ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΡ ΡΠ ΠΠ, ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ ΡΠ·Π½Π°Π²Π°ΡΡ ΠΎΠ±Π° Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΡΡ ΠΊΠΎ Π΄ΠΎΠ½Π° AAA ΠΈ AAG.
ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ ΠΈΠΌΠΏΠΎΡΡΠ° Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΠΏΠΎΠ΄ΡΠΎΠ±Π½ΠΎ ΠΈΠ·ΡΡΠ΅Π½Ρ, ΡΠ΅Π³ΠΎ Π½Π΅Π»ΡΠ·Ρ ΡΠΊΠ°Π·Π°ΡΡ ΠΎ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ°Ρ ΠΈΠΌΠΏΠΎΡΡΠ° Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ ΠΠ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΡ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ½ΠΎ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Ρ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ, Π½ΠΎ ΠΈ Ρ ΠΏΡΠΈΠΊΠ»Π°Π΄Π½ΠΎΠΉ ΡΠΎΡΠΊΠΈ Π·ΡΠ΅Π½ΠΈΡ. ΠΠΎ ΡΠ΅Π³ΠΎΠ΄Π½ΡΡΠ½Π΅Π³ΠΎ Π΄Π½Ρ Π² ΠΌΠΈΡΠ΅ Π½Π΅ ΡΡΡΠ΅ΡΡΠ²ΡΠ΅Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ Π²Π΅ΠΊΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π΄Π»Ρ Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. Π’Π°ΠΊΡΡ ΡΠΈΡΡΠ΅ΠΌΡ ΠΌΠΎΠΆΠ½ΠΎ Π±ΡΠ»ΠΎ Π±Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ Π² Π³Π΅Π½Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΡΠ΄Π° Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΡΡ ΠΌΡΡΠ°ΡΠΈΡΠΌΠΈ Π² Π³Π΅Π½Π°Ρ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΠ ΠΠ. Π€Π΅Π½ΠΎΠΌΠ΅Π½ ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΡΠΎΠ±ΠΎΠΉ Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΡΠΈΡΡΠ΅ΠΌΡ Π°Π΄ΡΠ΅ΡΠ½ΠΎΠΉ Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ. Π‘ ΡΡΠΎΠΉ ΡΠΎΡΠΊΠΈ Π·ΡΠ΅Π½ΠΈΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ ΠΎΡΠΎΠ±ΠΎ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ½ΡΠΌ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, Π² Π½ΠΎΡΠΌΠ΅ Π½Π΅ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΡΡΠΈΡ ΡΠ ΠΠ.
Π¦Π΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°.
Π Ρ ΠΎΠ΄Π΅ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π»ΠΎΡΡ Π²ΡΠΏΠΎΠ»Π½ΠΈΡΡ ΡΠ»Π΅Π΄ΡΡΡΠΈΠ΅ Π·Π°Π΄Π°ΡΠΈ:
1. ΠΡΠ΅Π½ΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π° Π½Π° ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΡΠ ΠΠ1 ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ.
2. ΠΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ, ΡΠΏΠΎΡΠΎΠ±Π½Π° Π»ΠΈ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠ°Ρ ΡΠ ΠΠ ΡΡΠ°ΡΡΠ²ΠΎΠ²Π°ΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ°.
3. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ ΡΠ ΠΠ ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°.
ΠΠ«ΠΠΠΠ«:
ΠΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π° ΡΠ ΠΠΠΠΈΠ· ΡΠ½ΠΈΠΆΠ°ΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΅Π΅ Π»ΠΈΠ·ΠΈΠ½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ, Π½ΠΎ Π½Π΅ ΠΏΡΠ΅ΠΏΡΡΡΡΠ²ΡΡΡ ΠΈΠΌΠΏΠΎΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ. ΠΠ΅ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΠ΅ Π²Π΅ΡΡΠΈΠΈ ΡΠ ΠΠΠ»ΠΈΠ· ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Π² Π΄Π΅Π°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΈΠ»ΠΈ ΠΌΠΈΡΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΠ΅.
Π¦ΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ ΠΠ, ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΠ΅ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ, ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΡΡΠ°ΡΡΠ²ΠΎΠ²Π°ΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ°. ΠΠ΅ΡΠ΅ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠ΅ (Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΠ΅) ΡΠ ΠΠ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΊΠ°ΠΊ in vitro, ΡΠ°ΠΊ ΠΈ in vivo. ΠΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ ΠΠ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ° Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΡΠ ΠΠ, ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΠ΅ ΠΈΠ· ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΡ, ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΡΠ°ΡΡΠΈΡΠ½ΠΎ ΠΊΠΎΠΌΠΏΠ΅Π½ΡΠΈΡΠΎΠ²Π°ΡΡ Π΄ΡΡ Π°ΡΠ΅Π»ΡΠ½ΡΡ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΡΡ ΠΌΡΡΠ°ΡΠΈΠ΅ΠΉ Π² Π³Π΅Π½Π΅, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ΅ΠΌ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΠ ΠΠ.
Π‘ΠΏΠΈΡΠΎΠΊ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
- Π‘ΠΎΠΉΠ΄Π»Π° Π’.Π . ΠΈ ΠΠΎΠ»ΠΎΠ²Π°Π½ΠΎΠ² Π.Π. 1984 ΠΠΎΠ·ΠΌΠΎΠΆΠ½Π°Ρ ΡΠΎΠ»Ρ TPHKlLys Π² ΡΠ·Π½Π°Π²Π°Π½ΠΈΠΈ ΡΡΠ°ΡΡΠΊΠΎΠ² ΠΏΡΠ΅-ΠΌΠ ΠΠ, ΠΈΠΌΠ΅ΡΡΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ Π΄Π»Ρ ΡΠΏΠ»Π°ΠΉΡΠΈΠ½Π³Π°. ΠΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½Π°Ρ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡ 18:277−285.
- Abe Y, Shodai Π’, Muto Π’, Mihara Π, Torii Π, Nishikawa S, Endo T, Kohda D. 2000. Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20. Cell 100(5):551−60.
- Adhya S, Ghosh T, Das A, Bera SK, Mahapatra S. 1997. Role of an RNA-binding protein in import of tRNA into Leishmania mitochondria. J Biol Chem 272(34):21 396−402.
- Akashi K, Hirayama J, Takenaka M, Yamaoka S, Suyama Y, Fukuzawa H, Ohyama K. 1997. Accumulation of nuclear-encoded tRNA (Thr) (AGU) in mitochondria of the liverwort Marchantia polymorpha. Biochim Biophys Acta 1350(3):262−6.
- Akashi K, Sakurai K, Hirayama J, Fukuzawa H, Ohyama K. 1996. Occurrence of nuclear-encoded tRNAIle in mitochondria of the liverwort Marchantia polymorpha. Curr Genet 30(2):181−5.
- Akashi K, Takenaka M, Yamaoka S, Suyama Y, Fukuzawa H, Ohyama K. 1998. Coexistence of nuclear DNA-encoded tRNAVal (AAC) and mitochondrial DNA-encoded tRNAVal (UAC) in mitochondria of a liverwort Marchantia polymorpha. Nucleic Acids Res 26(9):2168−72.
- Alfonzo JD, Blanc V, Estevez AM, Rubio MA, Simpson L. 1999. Π‘ to U editing of the anticodon of imported mitochondrial tRNA (Trp) allows decoding of the UGA stop codon in Leishmania tarentolae. Embo J 18(24):7056−62.
- Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon 1Π‘, Nierlich DP, Roe BA, Sanger F and others. 1981. Sequence and organization of the human mitochondrial genome. Nature 290(5806):457−65.
- Aphasizhev R, Senger B, Fasiolo F. 1997. Importance of structural features for tRNA (Met) identity. Rna 3(5):489−97.
- Baker KP, Schaniel A, Vestweber D, Schatz G. 1990. A yeast mitochondrial outer membrane protein essential for protein import and cell viability. Nature 348(6302):605−9.
- Bauer MF, Sirrenberg C, Neupert W, Brunner M. 1996. Role of Tim23 as voltage sensor and presequence receptor in protein import into mitochondria. Cell
- Beasley ΠΠ, Muller S, Schatz G. 1993. The signal that sorts yeast cytochrome b2 to the mitochondrial intermembrane space contains three distinct functional regions. Embo J 12(6):2303−11.
- Bhattacharyya SN, Chatterjee S, Adhya S. 2002. Mitochondrial RNA import in Leishmania tropica: aptamers homologous to multiple tRNA domains that interact cooperatively or antagonistically at the inner membrane. Mol Cell Biol 22(12):4372−82.
- Bhattacharyya SN, Mukherjee S, Adhya S. 2000. Mutations in a tRNA import signal define distinct receptors at the two membranes of Leishmania mitochondria. Mol Cell Biol 20(19):7410−7.
- Birnboim H C, Doly J. 1979 A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7(6): 1513−23.
- Borner GV, Mori M, Janke A, Paabo S. 1996. RNA editing changes the identity of a mitochondrial tRNA in marsupials. Embo J 15(21):5949−57.
- Brix J, Dietmeier K, Pfanner N. 1997. Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70. J Biol Chem 272(33):20 730−5.
- Brix J, Ziegler GA, Dietmeier K, Schneider-Mergener J, Schulz GE, Pfanner N. 2000. The mitochondrial import receptor Tom70: identification of a 25 kDa core domain with a specific binding site for preproteins. J Mol Biol 303(4):479−88.
- Chang DD, Clayton DA. 1987. A novel endoribonuclease cleaves at a priming site of mouse mitochondrial DNA replication. Embo J 6(2):409−17.
- Chen DH, Shi X, Suyama Y. 1994. In vivo expression and mitochondrial import of normal and mutated tRNA (thr) in Leishmania. Mol Biochem Parasitol 64(l):121−33.
- Chirico WJ, Waters MG, Blobel G. 1988. 70K heat shock related proteins stimulate protein translocation into microsomes. Nature 332(6167):805−10.
- Chiu N, Chiu A, Suyama Y. 1975. Native and imported transfer RNA in mitochondria. J Mol Biol 99(l):37−50.
- Commans S, Lazard M, Delort F, Blanquet S, Plateau P. 1998. tRNA anticodon recognition and specification within subclass lib aminoacyl-tRNA synthetases. J Mol Biol 278(4):801−13.
- Dekker PJ, Ryan MT, Brix J, Muller H, Honlinger A, Pfanner N. 1998. Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol Cell Biol 18(11):6515−24.
- Deshaies RJ, Koch BD, Werner-Washburne M, Craig EA, Schekman R. 1988. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature 332(6167): 800−5.
- Dietrich A, Marechal-Drouard L, Carneiro V, Cosset A, Small I. 1996a. A single base change prevents import of cytosolic tRNA (Ala) into mitochondria in transgenic plants. Plant J 10(5):913−8.
- Dietrich A, Small I, Cosset A, Weil JH, Marechal-Drouard L. 1996b. Editing and import: strategies for providing plant mitochondria with a complete set of functional transfer RNAs. Biochimie 78(6):518−29. Notes: Using Smart Source Parsing
- Dietrich A, Weil JH, Marechal-Drouard L. 1992. Nuclear-encoded transfer RNAs in plant mitochondria. Annu Rev Cell Biol 8:115−31. Notes: Using Smart Source Parsing
- Doersen CJ, Guerrier-Takada C, Altman S, Attardi G. 1985. Characterization of an RNase P activity from HeLa cell mitochondria. Comparison with the cytosol RNase P activity. J Biol Chem 260(10):5942−9.
- Donzeau M, Kaldi K, Adam A, Paschen S, Wanner G, Guiard B, Bauer MF, Neupert W, Brunner M. 2000. Tim23 links the inner and outer mitochondrial membranes. Cell 101(4):401−12.
- Dorner M, Altmann M, Paabo S, Mori M. 2001. Evidence for Import of a Lysyl-tRNA into Marsupial Mitochondria. Mol Biol Cell 12(9):2688−98.
- Eilers M And Schatz G. 1986 Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. Nature 322:228−232.
- Enriquez JA, Chomyn A, Attardi G. 1995b. MtDNA mutation in MERRF syndrome causes defective aminoacylation of tRNA (Lys) and premature
- Entelis NS, Kieffer S, Kolesnikova OA, Martin RP, Tarassov IA. 1998. Structural requirements of tRNALys for its import into yeast mitochondria. Proc Natl Acad Sci U S A 95(6):2838−43.
- Entelis NS, Kolesnikova OA, Dogan S, Martin RP, Tarassov IA. 2001. 5 S rRNA and tRNA Import into Human Mitochondria. COMPARISON OF IN VITRO REQUIREMENTS. J Biol Chem 276(49):45 642−53.
- Entelis NS, Krasheninnikov IA, Martin RP, Tarassov IA. 1996. Mitochondrial import of a yeast cytoplasmic tRNA (Lys): possible roles of aminoacylation and modified nucleosides in subcellular partitioning. FEBS Lett 384(l):38−42.
- Fey J, Weil JH, Tomita K, Cosset A, Dietrich A, Small I, Marechal-Drouard L. 2002 Role of editing in plant mitochondrial transfer RNAs. Gene. 286(l):21−4.
- Freist W, Gauss DH. 1995. Lysyl-tRNA synthetase. Biol Chem Hoppe Seyler 376(8):451−72.
- Fujiki M, Verner K. 1993. Coupling of cytosolic protein synthesis and mitochondrial protein import in yeast. Evidence for cotranslational import in vivo. J Biol Chem 268(3): 1914−20.
- Gartner F, Voos W, Querol A, Miller BR, Craig EA, Cumsky MG, Pfanner N. 1995. Mitochondrial import of subunit Va of cytochrome Ρ oxidase characterized with yeast mutants. J Biol Chem 270(8):3788−95.
- Giege R, Puglisi JD, Florentz C. 1993. tRNA structure and aminoacylation efficiency. Prog Nucleic Acid Res Mol Biol 45:129−206. Notes: Using Smart Source Parsing
- Glick BS, Brandt A, Cunningham K, Muller S, Hallberg RL, Schatz G. 1992. Cytochromes cl and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism. Cell 69(5):809−22.
- Glover KE, Spencer DF, Gray MW. 2001. Identification and structural characterization of nucleus-encoded transfer RNAs imported into wheat mitochondria. J Biol Chem 276(l):639−48.
- Goto Y, Nonaka I, Horai S. 1990. A mutation in the tRNA (Leu)(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies. Nature 348(6302):651−3.
- Gray MW and Boyer PH. 1988 Organization and expression of algal (Chlamydomonas reinhardtii) mitochondrial DNA. Philos Trans R Soc Lond Π
- Biol Sci. 319(1193): 135−47.
- Hachiya N, Komiya T, Alam R, Iwahashi J, Sakaguchi M, Omura T, Mihara K. 1994. MSF, a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria. EMBO J 13(21):5146−54.
- Hancock K, Hajduk SL. 1990. The mitochondrial tRNAs of Trypanosoma brucei are nuclear encoded. J Biol Chem 265(31):19 208−15.
- Hancock K, LeBlanc AJ, Donze D, Hajduk SL. 1992. Identification of nuclear encoded precursor tRNAs within the mitochondrion of Trypanosoma brucei. J Biol Chem 267(33):23 963−71.
- Hauser R, Schneider A. 1995. tRNAs are imported into mitochondria of Trypanosoma brucei independently of their genomic context and genetic origin. Embo J 14(17):4212−20.
- Hayashi J, Ohta S, Kikuchi A, Takemitsu M, Goto Y, Nonaka I. 1991. Introduction of disease-related mitochondrial DNA deletions into HeLa cells lacking mitochondrial DNA results in mitochondrial dysfunction. Proc Natl Acad Sci US A 88(23): 10 614−8.
- Heitzler J, Marechal-Drouard L, Dirheimer G, Keith G. 1992. Use of a dot blot hybridization method for identification of pure tRNA species on different membranes. Biochim Biophys Acta 1129(3):273−7.
- Hell K, Herrmann J, Pratje E, Neupert W, Stuart RA. 1997. Oxalp mediates the export of the N- and C-termini of pCoxII from the mitochondrial matrix to the intermembrane space. FEBS Lett 418(3):367−70.
- Hell K, Herrmann JM, Pratje E, Neupert W, Stuart RA. 1998. Oxalp, an essential component of the N-tail protein export machinery in mitochondria. Proc Natl Acad Sci U S A 95(5):2250−5.
- Hell K, Neupert W, Stuart RA. 2001. Oxalp acts as a general membrane insertion machinery for proteins encoded by mitochondrial DNA. EMBO J 20(6): 1281−8.
- Helm M, Brule H, Degoul F, Cepanec C, Leroux JP, Giege R, Florentz C. 1998. The presence of modified nucleotides is required for cloverleaf folding of a human mitochondrial tRNA. Nucleic Acids Res 26(7): 163 6−43.
- Helm M, Florentz C, Chomyn A, Attardi G. 1999. Search for differences in post-transcriptional modification patterns of mitochondrial DNA-encoded wild-type and mutant human tRNALys and tRNALeu (UUR). Nucleic Acids Res 27(3):756−63.
- Hill J., Donald KA., Griffiths DE, Donald G. 1991 DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res. 19(20):5791.
- Hill K, Model K, Ryan MT, Dietmeier K, Martin F, Wagner R, Pfanner N. 1998. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature 395(6701):516−21.
- Honlinger A, Bomer U, Alconada A, Eckerskorn Π‘, Lottspeich F, Dietmeier Π, Pfanner N. 1996. Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J 15(9):2125−37.
- Horwich AL, Kalousek F, Mellman I, Rosenberg LE. 1985. A leader peptide is sufficient to direct mitochondrial import of a chimeric protein. EMBO J 4(5): 1129−35.
- Hou YM, Schimmel P. 1988. A simple structural feature is a major determinant of the identity of a transfer RNA. Nature 333(6169):140−5.
- Huang S, Ratliff KS, Schwartz MP, Spenner JM, Matouschek A. 1999. Mitochondria unfold precursor proteins by unraveling them from their N- termini. Nat Struct Biol 6(12): 1132−8.
- Hurt EC, Pesold-Hurt B, Schatz G. 1984. The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix. FEBS Lett 178(2):306−10.
- James AM, Wei YH, Pang CY, Murphy MP. 1996. Altered mitochondrial function in fibroblasts containing MELAS or MERRF mitochondrial DNA mutations. Biochem J 318 (Pt 2):401−7.
- Janke A, Xu X, Arnason U. 1997. The complete mitochondrial genome of the wallaroo (Macropus robustus) and the phylogenetic relationship among Monotremata, Marsupialia, and Eutheria. Proc Natl Acad Sci U S A 94(4): 127 681.
- Kapushoc ST, Alfonzo JD, Rubio MA, Simpson L. 2000. End processing precedes mitochondrial importation and editing of tRNAs in leishmania tarentolae. J Biol Chem 275(48):37 907−14.
- Kazakova HA, Entelis NS, Martin RP, Tarassov IA. 1999. The aminoacceptor stem of the yeast tRNA (Lys) contains determinants of mitochondrial import selectivity. FEBS Lett 442(2−3): 193−7.
- Kellems RE, Allison VF, Butow RA. 1975. Cytoplasmic type 80S ribosomes associated with yeast mitochondria. IV. Attachment of ribosomes to the outer membrane of isolated mitochondria. J Cell Biol 65(1): 1−14.
- Kerscher O, Sepuri NB, Jensen RE. 2000. Timl8p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol Biol Cell 11(1):103−16.
- King MP, Attardi G. 1988. Injection of mitochondria into human cells leads to a rapid replacement of the endogenous mitochondrial DNA. Cell 52(6):811−9.
- Knox Π‘, Sass Π, Neupert W, Pines Π. 1998. Import into mitochondria, folding and retrograde movement of fumarase in yeast. J Biol Chem 273(40):25 587−93.
- Koehler CM, Jarosch E, Tokatlidis K, Schmid K, Schweyen RJ, Schatz G. 1998. Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. Science 279(5349):369−73.
- Kolesnikova OA, Entelis NS, Mireau H, Fox TD, Martin RP, Tarassov IA. 2000 Suppression of mutations in mitochondrial DNA by tRNAs imported from the cytoplasm. Science 289(5486): 1931−3.
- Komiya T, Rospert S, Koehler C, Looser R, Schatz G, Mihara K. 1998. Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway: evidence for the 'acid chain' hypothesis. EMBO J 17(14):3886−98.
- Kumar R, Marechal-Drouard L, Akama K, Small I. 1996. Striking differences in mitochondrial tRNA import between different plant species. Mol Gen Genet 252(4):404-ll.
- Kunkel Π’Π, Roberts JD, Zakour RA. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367−82. Notes: Using Smart Source Parsing
- Kunkele KP, Heins S, Dembowski M, Nargang FE, Benz R, Thieffry M, Walz J, Lill R, Nussberger S, Neupert W. 1998. The preprotein translocation channel of the outer membrane of mitochondria. Cell 93(6): 1009−19.
- Kurz M, Martin H, Rassow J, Pfanner N, Ryan MT. 1999. Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway. Mol Biol Cell 10(7):2461−74.
- LeBlanc AJ, Yermovsky-Kammerer AE, Hajduk SL. 1999. A nuclear encoded and mitochondrial imported dicistronic tRNA precursor in Trypanosoma brucei. J Biol Chem 274(30):21 071−7.
- Lee CM, Sedman J, Neupert W, Stuart RA. 1999. The DNA helicase, Hmilp, is transported into mitochondria by a C- terminal cleavable targeting signal. J Biol Chem 274(30):20 937−42.
- Li K, Smagula CS, Parsons WJ, Richardson JA, Gonzalez M, Hagler HK,
- Williams RS. 1994. Subcellular partitioning of MRP RNA assessed by ultrastructural and biochemical analysis. J Cell Biol 124(6):871−82.
- Lima BD, Simpson L. 1996. Sequence-dependent in vivo importation of tRNAs into the mitochondrion of Leishmania tarentolae. Rna 2(5):429−40.
- Lindsley D and Gallant J. 1993 On the directional specificity of ribosome frameshifting at a «hungry» codon. Proc Natl Acad Sci USA. 90(12): 5469−73.
- Lithgow T. 2000. Targeting of proteins to mitochondria. FEBS Lett 476(1−2):22−6.
- Lohan AJ and Wolfe KH. 1998 A subset of conserved tRNA genes in plastid DNA of nongreen plants. Genetics. 150(l):425−33.
- Lye LF, Chen DH, Suyama Y. 1993. Selective import of nuclear-encoded tRNAs into mitochondria of the protozoan Leishmania tarentolae. Mol Biochem Parasitol 58(2):233−45.
- Magalhaes PJ, Andreu AL, Schon EA. 1998. Evidence for the presence of 5S rRNA in mammalian mitochondria. Mol Biol Cell 9(9):2375−82.
- Mahapatra S, Adhya S. 1996. Import of RNA into Leishmania mitochondria occurs through direct interaction with membrane-bound receptors. J Biol Chem 271(34):20 432−7.
- Mahapatra S, Ghosh S, Bera SK, Ghosh T, Das A, Adhya S. 1998. The D arm of tRNATyr is necessary and sufficient for import into Leishmania mitochondria in vitro. Nucleic Acids Res 26(9):2037−41.
- Marechal-Drouard L, Guillemaut P, Cosset A, Arbogast M, Weber F, Weil JH, Dietrich A. 1990. Transfer RNAs of potato (Solanum tuberosum) mitochondria have different genetic origins. Nucleic Acids Res 18(13):3689−96.
- Martin RP, Dirheimer G. 1983. Two-dimensional polyacrylamide gel electrophoresis in the study of yeast mitochondrial transfer RNA. Mol Biol (Mosk) 17(6):1117−25.
- Martin RP, Schneller JM, Stahl AJ, Dirheimer G. 1979. Import of nuclear deoxyribonucleic acid coded lysine-accepting transfer ribonucleic acid (anticodon C-U-U) into yeast mitochondria. Biochemistry 18(21):4600−5.
- Masucci JP, Davidson M, Koga Y, Schon EA, King MP. 1995. In vitro analysis of mutations causing myoclonus epilepsy with ragged- red fibers in the mitochondrial tRNA (Lys)gene: two genotypes produce similar phenotypes. Mol Cell Biol 15(5):2872−81.
- Matouschek A, Azem A, Ratliff K, Glick BS, Schmid K, Schatz G. 1997. Active
- McClain WH, Foss K, Jenkins RA, Schneider J. 1990. Nucleotides that determine Escherichia coli tRNA (Arg) and tRNA (Lys) acceptor identities revealed by analyses of mutant opal and amber suppressor tRNAs. Proc Natl Acad Sci U S A 87(23):9260−4.
- McKee EE, Poyton RO. 1984. Mitochondrial gene expression in saccharomyces cerevisiae. I. Optimal conditions for protein synthesis in isolated mitochondria. J Biol Chem 259(14):9320−31.
- Mihara K, Omura T. 1996. Cytosolic factors in mitochondrial protein import. Experientia 52(12): 1063−8.
- Mireau H, Cosset A, Marechal-Drouard L, Fox TD, Small ID, Dietrich A. 2000. Expression of Arabidopsis thaliana mitochondrial alanyl-tRNA synthetase is not sufficient to trigger mitochondrial import of tRNAAla in yeast. J Biol Chem 275(18):13 291−6.
- Moczko M, Ehmann B, Gartner F, Honlinger A, Schafer E, Pfanner N. 1994. Deletion of the receptor MOM 19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J Biol Chem 269(12):9045−51.
- Moore M, Harrison MS, Peterson EC, Henry R. 2000. Chloroplast Oxalp homolog albino3 is required for post-translational integration of the light harvesting chlorophyll-binding protein into thylakoid membranes. J Biol Chem 275(3): 1529−32.
- Mukherjee S, Bhattacharyya SN, Adhya S. 1999. Stepwise transfer of tRNA through the double membrane of Leishmania mitochondria. J Biol Chem 274(44):31 249−55.
- Nameki N, Asahara H, Tamura K, Himeno H, Hasegawa T, Shimizu M. 1995. Similarities and differences in tRNA identity between Escherichia coli and Saccharomyces cerevisiae: evolutionary conservation and divergence. Nucleic Acids Symp Ser 34:205−6.
- Nargang FE, Preuss M, Neupert W, Herrmann JM. 2002. The Oxal protein forms a homooligomeric complex and is an essential part of the mitochondrial export translocase in Neurospora crassa. J Biol Chem 277(15):12 846−53.
- Neupert W. 1997. Protein import into mitochondria. Annu Rev Biochem 66:863 917.
- Pfanner N, Geissler A. 2001b. Versatility of the mitochondrial protein import machinery. Nat Rev Mol Cell Biol 2(5):339−49.
- Preuss M, Leonhard K, Hell K, Stuart RA, Neupert W, Herrmann JM. 2001. Mbal, a novel component of the mitochondrial protein export machinery of the yeast Saccharomyces cerevisiae. J Cell Biol 153(5):1085−96.
- Pritchard, Π Π, Seilhamer JJ, Mahalingam R, Sable CL, Venuti SE, Cummings DJ. 1990 Nucleotide sequence of the mitochondrial genome of Paramecium. Nucleic Acids Res. 18(l):173−80.
- Prokisch H, Nussberger S, Westermann B. 2002. Protein import into mitochondria of Neurospora crassa. Fungal Genet Biol 36(2):85−90.
- Reid GA, Schatz G. 1982. Import of proteins into mitochondria. Extramitochondrial pools and post- translational import of mitochondrial protein precursors in vivo. J Biol Chem 257(21):13 062−7.
- Rich A, RajBhandary UL. 1976. Transfer RNA: molecular structure, sequence, and properties. Annu Rev Biochem 45:805−60.
- Rojo EE, Guiard B, Neupert W, Stuart RA. 1998. Sorting of D-lactate dehydrogenase to the inner membrane of mitochondria. Analysis of topogenic signal and energetic requirements. J Biol Chem 273(14):8040−7.
- Rojo EE, Stuart RA, Neupert W. 1995. Conservative sorting of FO-ATPase subunit 9: export from matrix requires delta pH across inner membrane and matrix ATP. EMBO J 14(14):3445−51.
- Rospert S, Junne T, Glick BS, Schatz G. 1993. Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES. FEBS Lett 335(3):358−60.
- Rubio MA, Liu X, Yuzawa H, Alfonzo JD, Simpson L. 2000. Selective importation of RNA into isolated mitochondria from Leishmania tarentolae. Rna 6(7):988−1003.
- Rusconi CP, Cech TR. 1996a. The anticodon is the signal sequence for mitochondrial import of glutamine tRNA in Tetrahymena. Genes Dev 10(22):2870−80.
- Rusconi CP, Cech TR. 1996b. Mitochondrial import of only one of three nuclear-encoded glutamine tRNAs in Tetrahymena thermophila. Embo J 15(13):3286−95.
- Saracco SA, Fox TD. 2002. Coxl8p is required for export of the mitochondrially encoded Saccharomyces cerevisiae Cox2p C-tail and interacts with Pntlp and Mss2p in the inner membrane. Mol Biol Cell 13(4):1122−31.
- Saraste M. 1999. Oxidative phosphorylation at the fin de siecle. Science 283(5407): 1488−93.
- Schatz G, Dobberstein B. 1996. Common principles of protein translocation across membranes. Science 271(5255): 1519−26.
- Schleiff Π, Turnbull JL. 1998Π°. Characterization of the N-terminal targeting signal binding domain of the mitochondrial outer membrane receptor, Tom20. Biochemistry 37(38): 13 052−8.
- Schleiff E, Turnbull JL. 1998b. Functional and structural properties of the mitochondrial outer membrane receptor Tom20. Biochemistry 37(38): 13 043−51.
- Schneider A. 1996. Cytosolic yeast tRNA (His) is covalently modified when imported into mitochondria of Trypanosoma brucei. Nucleic Acids Res 24(7): 1225−8.
- Schneider A, Marechal-Drouard L. 2000. Mitochondrial tRNA import: are there distinct mechanisms? Trends Cell Biol 10(12):509−13.
- Schneider A, Martin J, Agabian N. 1994a. A nuclear encoded tRNA of Trypanosoma brucei is imported into mitochondria. Mol Cell Biol 14(4):2317−22.
- Schneider A, McNally KP, Agabian N. 1993 Splicing and Π'-processing of the tyrosine tRNA of Trypanosoma brucei. J Biol Chem. 268(29):21 868−74.
- Schneider A, McNally KP, Agabian N. 1994b. Nuclear-encoded mitochondrial tRNAs of Trypanosoma brucei have a modified cytidine in the anticodon loop. Nucleic Acids Res 22(18):3699−705.
- Scotti PA, Urbanus ML, Brunner J, de Gier JW, von Heijne G, van der Does C, Driessen AJ, Oudega B, Luirink J. 2000. YidC, the Escherichia coli homologue of mitochondrial Oxalp, is a component of the Sec translocase. EMBO J 19(4):542−9.
- Shi X, Chen DH, Suyama Y. 1994. A nuclear tRNA gene cluster in the protozoan Leishmania tarentolae and differential distribution of nuclear-encoded tRNAs between the cytosol and mitochondria. Mol Biochem Parasitol 65(l):23−37.
- Shiba K, Stello T, Motegi H, Noda T, Musier-Forsyth K, Schimmel P. 1997. Human lysyl-tRNA synthetase accepts nucleotide 73 variants and rescues Escherichia coli double-defective mutant. J Biol Chem 272(36):22 809−16.
- Sherratt HSA, Lightowlers RN, Turnbull DM.1997 in Organelle Disease, edited by Applegarth DA, Dimmick JE, Hall JG. Chapman and Hall, London
- Sikorski RS, Hieter P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122(1): 19−27.
- Sirrenberg C, Bauer MF, Guiard B, Neupert W, Brunner M. 1996. Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22.
- Sirrenberg Π‘, Endres Π, Folsch Π, Stuart RA, Neupert W, Brunner M. 1998. Carrier protein import into mitochondria mediated by the intermembrane proteins TimlO/Mrsll and Timl2/Mrs5. Nature 391(6670):912−5.
- Smith CJ, Ley AN, D’Obrenan P, Mitra SK. 1971. The structure and coding specificity of a lysine transfer ribonucleic acid from the haploid yeast Saccharomyces cerevisiae alpha S288C. J Biol Chem 246(24):7817−9.
- Sollner T, Griffiths G, Pfaller R, Pfanner N, Neupert W. 1989. MOM19, an import receptor for mitochondrial precursor proteins. Cell 59(6): 1061−70.
- Sollner T, Rassow J, Pfanner N. 1991. Analysis of mitochondrial protein import using translocation intermediates and specific antibodies. Methods Cell Biol 34:345−58.
- Notes: Using Smart Source Parsing
- Suyama Y. 1967. The origins of mitochondrial ribonucleic acids in Tetrahymena pyriformis. Biochemistry 6(9):2829−39.
- Suyama Y, Eyer J. 1967. Leucyl tRNA and leucyl tRNA synthetase in mitochondria of Tetrahymena pyriformis. Biochem Biophys Res Commun 28(5):746−51.
- Suyama Y, Hamada J. 1978. The mitochondrial and cytoplasmic valyl tRNA synthetases in Tetrahymena are indistinguishable. Arch Biochem Biophys 191(2):437−43.
- Tamura K, Himeno H, Asahara H, Hasegawa T, Shimizu M. 1992. In vitro study of E. coli tRNA (Arg) and tRNA (Lys) identity elements. Nucleic Acids Res 20(9):2335−9.
- Tan TH, Bochud-Allemann N, Horn EK, Schneider A. 2002a. Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria. Proc Natl Acad Sci U S A 99(3): 1152−7.
- Tan TH, Pach R, Crausaz A, Ivens A, Schneider A. 2002b. tRNAs in Trypanosoma brucei: genomic organization, expression, and mitochondrial import. Mol Cell Biol 22(11):3707−17.
- Tarassov I, Entelis N, Martin RP. 1995a. An intact protein translocating machinery is required for mitochondrial import of a yeast cytoplasmic tRNA. J Mol Biol 245(4):315−23.
- Tarassov I, Entelis N, Martin RP. 1995b. Mitochondrial import of a cytoplasmic lysine-tRNA in yeast is mediated by cooperation of cytoplasmic and
- Tarassov IA, Entelis NS. 1992. Mitochondrially-imported cytoplasmic tRNA (Lys)(CUU) of Saccharomyces cerevisiae: in vivo and in vitro targetting systems. Nucleic Acids Res 20(6): 1277−81.
- Tarassov IA, Entelis NS, Martin RP. 1999. Import of tRNA into yeast mitochondria: experimental approaches and possible applications. Lestienne P Heidelberg: Springer, p 303−16.
- Varshney U, Lee CP, RajBhandary UL. 1991. Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase. J Biol Chem 266(36):24 712−8.
- Wallace DC. 1999. Mitochondrial diseases in man and mouse. Science 283(5407): 1482−8.
- Wiedemann N, Pfanner N, Ryan MT. 2001. The three modules of ADP/ATP carrier cooperate in receptor recruitment and translocation into mitochondria. Embo J 20(5):951−60.
- Wienhues U, Becker K, Schleyer M, Guiard B, Tropschug M, Horwich AL, Pfanner N, Neupert W. 1991. Protein folding causes an arrest of preprotein translocation into mitochondria in vivo. J Cell Biol 115(6):1601−9.
- Yasukawa T, Suzuki T, Ishii N, Ohta S, Watanabe K. 2001. Wobble modification defect in tRNA disturbs codon-anticodon interaction in a mitochondrial disease. Embo J 20(17):4794−802.
- Yasukawa T, Suzuki T, Ishii N, Ueda T, Ohta S, Watanabe K. 2000. Defect in modification at the anticodon wobble nucleotide of mitochondrial tRNA (Lys) with the MERRF encephalomyopathy pathogenic mutation. FEBS Lett 467(2−3):175−8.
- Yermovsky-Kammerer AE, Hajduk SL. 1999. In vitro import of a nuclearlyencoded tRNA into the mitochondrion of Trypanosoma brucei. Mol Cell Biol 19(9):6253−9.
- Yoshionari S, Koike T, Yokogawa T, Nishikawa K, Ueda T, Miura K, Watanabe K. 1994. Existence of nuclear-encoded 5S-rRNA in bovine mitochondria. FEBS Lett 338(2): 137−42.